PubMed:10814704 JSONTXT

{"project":"GlyCosmos6-UBERON","denotations":[{"id":"T1","span":{"begin":134,"end":138},"obj":"Body_part"},{"id":"T2","span":{"begin":1072,"end":1079},"obj":"Body_part"},{"id":"T3","span":{"begin":1400,"end":1404},"obj":"Body_part"},{"id":"T4","span":{"begin":1589,"end":1593},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000062"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000000"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":557,"end":562},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos600-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":557,"end":562},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos600-CLO","denotations":[{"id":"T1","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T2","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T3","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T4","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T5","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T6","span":{"begin":134,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T8","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T9","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T10","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T11","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T12","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T13","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T14","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T15","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T16","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T17","span":{"begin":571,"end":576},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T19","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T20","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T21","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T22","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T23","span":{"begin":706,"end":711},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T24","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T25","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T26","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T27","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T28","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T29","span":{"begin":1348,"end":1353},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T30","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T31","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T32","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T33","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T34","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T35","span":{"begin":1400,"end":1404},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T36","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T37","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T38","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T39","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T40","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T41","span":{"begin":1589,"end":1593},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":30,"end":35},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"NCBItxid:9606"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":557,"end":562},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":557,"end":562},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":144},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":145,"end":432},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":433,"end":482},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":483,"end":577},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":578,"end":712},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":713,"end":879},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":880,"end":1066},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1067,"end":1199},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1200,"end":1354},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1355,"end":1439},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1440,"end":1610},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":144},"obj":"Sentence"},{"id":"T2","span":{"begin":145,"end":432},"obj":"Sentence"},{"id":"T3","span":{"begin":433,"end":482},"obj":"Sentence"},{"id":"T4","span":{"begin":483,"end":577},"obj":"Sentence"},{"id":"T5","span":{"begin":578,"end":712},"obj":"Sentence"},{"id":"T6","span":{"begin":713,"end":879},"obj":"Sentence"},{"id":"T7","span":{"begin":880,"end":1066},"obj":"Sentence"},{"id":"T8","span":{"begin":1067,"end":1199},"obj":"Sentence"},{"id":"T9","span":{"begin":1200,"end":1354},"obj":"Sentence"},{"id":"T10","span":{"begin":1355,"end":1439},"obj":"Sentence"},{"id":"T11","span":{"begin":1440,"end":1610},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos6-CLO","denotations":[{"id":"T1","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T2","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T3","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T4","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T5","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T6","span":{"begin":134,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T8","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T9","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T10","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T11","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T12","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T13","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T14","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T15","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T16","span":{"begin":565,"end":570},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T17","span":{"begin":571,"end":576},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T19","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T20","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T21","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T22","span":{"begin":700,"end":705},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T23","span":{"begin":706,"end":711},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T24","span":{"begin":1330,"end":1338},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T25","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T26","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T27","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T28","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T29","span":{"begin":1342,"end":1347},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T30","span":{"begin":1348,"end":1353},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T31","span":{"begin":1364,"end":1372},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T32","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T33","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T34","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T35","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T36","span":{"begin":1394,"end":1399},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T37","span":{"begin":1400,"end":1404},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T38","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0003775"},{"id":"T39","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051930"},{"id":"T40","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051932"},{"id":"T41","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051933"},{"id":"T42","span":{"begin":1583,"end":1588},"obj":"http://purl.obolibrary.org/obo/CLO_0051934"},{"id":"T43","span":{"begin":1589,"end":1593},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":93,"end":97},"obj":"FMAID:198663"},{"id":"_T2","span":{"begin":422,"end":426},"obj":"FMAID:198073"},{"id":"_T3","span":{"begin":422,"end":426},"obj":"FMAID:84120"},{"id":"_T4","span":{"begin":571,"end":576},"obj":"FMAID:68646"},{"id":"_T5","span":{"begin":571,"end":576},"obj":"FMAID:169002"},{"id":"_T6","span":{"begin":656,"end":660},"obj":"FMAID:198663"},{"id":"_T7","span":{"begin":706,"end":711},"obj":"FMAID:68646"},{"id":"_T8","span":{"begin":706,"end":711},"obj":"FMAID:169002"},{"id":"_T9","span":{"begin":759,"end":763},"obj":"FMAID:198663"},{"id":"_T10","span":{"begin":780,"end":787},"obj":"FMAID:84116"},{"id":"_T11","span":{"begin":780,"end":787},"obj":"FMAID:198062"},{"id":"_T12","span":{"begin":982,"end":992},"obj":"FMAID:82740"},{"id":"_T13","span":{"begin":982,"end":992},"obj":"FMAID:196729"},{"id":"_T14","span":{"begin":1054,"end":1061},"obj":"FMAID:198062"},{"id":"_T15","span":{"begin":1054,"end":1061},"obj":"FMAID:84116"},{"id":"_T16","span":{"begin":1348,"end":1353},"obj":"FMAID:68646"},{"id":"_T17","span":{"begin":1348,"end":1353},"obj":"FMAID:169002"},{"id":"_T18","span":{"begin":1571,"end":1575},"obj":"FMAID:198663"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":61,"end":80},"obj":"http://www.uniprot.org/uniprot/Q8IX54"},{"id":"T2","span":{"begin":128,"end":130},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T3","span":{"begin":271,"end":273},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T4","span":{"begin":565,"end":567},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T5","span":{"begin":700,"end":702},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T6","span":{"begin":1342,"end":1344},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T7","span":{"begin":1394,"end":1396},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T8","span":{"begin":1583,"end":1585},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T9","span":{"begin":128,"end":130},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T10","span":{"begin":271,"end":273},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T11","span":{"begin":565,"end":567},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T12","span":{"begin":700,"end":702},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T13","span":{"begin":1342,"end":1344},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T14","span":{"begin":1394,"end":1396},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T15","span":{"begin":1583,"end":1585},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T16","span":{"begin":128,"end":130},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T17","span":{"begin":271,"end":273},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T18","span":{"begin":565,"end":567},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T19","span":{"begin":700,"end":702},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T20","span":{"begin":1342,"end":1344},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T21","span":{"begin":1394,"end":1396},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T22","span":{"begin":1583,"end":1585},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T23","span":{"begin":383,"end":385},"obj":"http://www.uniprot.org/uniprot/P09131"},{"id":"T24","span":{"begin":810,"end":812},"obj":"http://www.uniprot.org/uniprot/P09131"},{"id":"T25","span":{"begin":1029,"end":1031},"obj":"http://www.uniprot.org/uniprot/P09131"},{"id":"T26","span":{"begin":1119,"end":1121},"obj":"http://www.uniprot.org/uniprot/Q6I9R6"},{"id":"T27","span":{"begin":1467,"end":1469},"obj":"http://www.uniprot.org/uniprot/Q6I9R6"},{"id":"T28","span":{"begin":1119,"end":1121},"obj":"http://www.uniprot.org/uniprot/P38435"},{"id":"T29","span":{"begin":1467,"end":1469},"obj":"http://www.uniprot.org/uniprot/P38435"},{"id":"T30","span":{"begin":1119,"end":1121},"obj":"http://www.uniprot.org/uniprot/P02774"},{"id":"T31","span":{"begin":1467,"end":1469},"obj":"http://www.uniprot.org/uniprot/P02774"},{"id":"T32","span":{"begin":1155,"end":1160},"obj":"http://www.uniprot.org/uniprot/Q9BPV1"},{"id":"T33","span":{"begin":1237,"end":1242},"obj":"http://www.uniprot.org/uniprot/Q9BPV1"},{"id":"T34","span":{"begin":1284,"end":1289},"obj":"http://www.uniprot.org/uniprot/Q9BPV1"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":61,"end":80},"obj":"http://www.uniprot.org/uniprot/P97325"},{"id":"T2","span":{"begin":128,"end":130},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T3","span":{"begin":271,"end":273},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T4","span":{"begin":565,"end":567},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T5","span":{"begin":700,"end":702},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T6","span":{"begin":1342,"end":1344},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T7","span":{"begin":1394,"end":1396},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T8","span":{"begin":1583,"end":1585},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T9","span":{"begin":128,"end":130},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T10","span":{"begin":271,"end":273},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T11","span":{"begin":565,"end":567},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T12","span":{"begin":700,"end":702},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T13","span":{"begin":1342,"end":1344},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T14","span":{"begin":1394,"end":1396},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T15","span":{"begin":1583,"end":1585},"obj":"http://www.uniprot.org/uniprot/P38060"},{"id":"T16","span":{"begin":383,"end":385},"obj":"http://www.uniprot.org/uniprot/P21129"},{"id":"T17","span":{"begin":810,"end":812},"obj":"http://www.uniprot.org/uniprot/P21129"},{"id":"T18","span":{"begin":1029,"end":1031},"obj":"http://www.uniprot.org/uniprot/P21129"},{"id":"T19","span":{"begin":1001,"end":1003},"obj":"http://www.uniprot.org/uniprot/Q99PJ0"},{"id":"T20","span":{"begin":1114,"end":1117},"obj":"http://www.uniprot.org/uniprot/Q76LW6"},{"id":"T21","span":{"begin":1229,"end":1232},"obj":"http://www.uniprot.org/uniprot/Q76LW6"},{"id":"T22","span":{"begin":1265,"end":1268},"obj":"http://www.uniprot.org/uniprot/Q76LW6"},{"id":"T23","span":{"begin":1114,"end":1117},"obj":"http://www.uniprot.org/uniprot/O89090"},{"id":"T24","span":{"begin":1229,"end":1232},"obj":"http://www.uniprot.org/uniprot/O89090"},{"id":"T25","span":{"begin":1265,"end":1268},"obj":"http://www.uniprot.org/uniprot/O89090"},{"id":"T26","span":{"begin":1155,"end":1160},"obj":"http://www.uniprot.org/uniprot/Q9WTZ4"},{"id":"T27","span":{"begin":1237,"end":1242},"obj":"http://www.uniprot.org/uniprot/Q9WTZ4"},{"id":"T28","span":{"begin":1284,"end":1289},"obj":"http://www.uniprot.org/uniprot/Q9WTZ4"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":36,"end":40},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T2","span":{"begin":36,"end":40},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T3","span":{"begin":193,"end":197},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T4","span":{"begin":193,"end":197},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T5","span":{"begin":571,"end":576},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T6","span":{"begin":706,"end":711},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T7","span":{"begin":1348,"end":1353},"obj":"http://purl.bioontology.org/ontology/STY/T025"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":0,"end":15},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T2","span":{"begin":341,"end":356},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T3","span":{"begin":616,"end":631},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T4","span":{"begin":1314,"end":1329},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T5","span":{"begin":1527,"end":1542},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T6","span":{"begin":16,"end":26},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T7","span":{"begin":357,"end":367},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T8","span":{"begin":487,"end":497},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T9","span":{"begin":632,"end":642},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T10","span":{"begin":1543,"end":1553},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T11","span":{"begin":341,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0006355"},{"id":"T12","span":{"begin":443,"end":445},"obj":"http://purl.obolibrary.org/obo/GO_0004306"},{"id":"T13","span":{"begin":550,"end":556},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T14","span":{"begin":759,"end":774},"obj":"http://purl.obolibrary.org/obo/GO_0010467"},{"id":"T15","span":{"begin":1400,"end":1420},"obj":"http://purl.obolibrary.org/obo/GO_0030154"},{"id":"T16","span":{"begin":1589,"end":1609},"obj":"http://purl.obolibrary.org/obo/GO_0030154"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":134,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":1400,"end":1404},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":571,"end":576},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":706,"end":711},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5","span":{"begin":1348,"end":1353},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":0,"end":15},"obj":"http://edamontology.org/topic_3308"},{"id":"T2","span":{"begin":0,"end":15},"obj":"http://edamontology.org/topic_3512"},{"id":"T3","span":{"begin":0,"end":15},"obj":"http://edamontology.org/topic_0110"},{"id":"T4","span":{"begin":0,"end":15},"obj":"http://edamontology.org/topic_0203"},{"id":"T5","span":{"begin":30,"end":35},"obj":"http://edamontology.org/topic_2815"},{"id":"T6","span":{"begin":341,"end":356},"obj":"http://edamontology.org/topic_3512"},{"id":"T7","span":{"begin":341,"end":356},"obj":"http://edamontology.org/topic_0110"},{"id":"T8","span":{"begin":341,"end":356},"obj":"http://edamontology.org/topic_0203"},{"id":"T9","span":{"begin":341,"end":356},"obj":"http://edamontology.org/topic_3308"},{"id":"T10","span":{"begin":386,"end":394},"obj":"http://edamontology.org/topic_0749"},{"id":"T11","span":{"begin":386,"end":394},"obj":"http://edamontology.org/topic_0111"},{"id":"T12","span":{"begin":386,"end":394},"obj":"http://edamontology.org/topic_1312"},{"id":"T13","span":{"begin":422,"end":426},"obj":"http://edamontology.org/topic_2397"},{"id":"T14","span":{"begin":422,"end":426},"obj":"http://edamontology.org/topic_3512"},{"id":"T15","span":{"begin":604,"end":611},"obj":"http://edamontology.org/topic_3678"},{"id":"T16","span":{"begin":616,"end":631},"obj":"http://edamontology.org/topic_0203"},{"id":"T17","span":{"begin":616,"end":631},"obj":"http://edamontology.org/topic_3308"},{"id":"T18","span":{"begin":616,"end":631},"obj":"http://edamontology.org/topic_3512"},{"id":"T19","span":{"begin":616,"end":631},"obj":"http://edamontology.org/topic_0110"},{"id":"T20","span":{"begin":759,"end":774},"obj":"http://edamontology.org/topic_0203"},{"id":"T21","span":{"begin":780,"end":787},"obj":"http://edamontology.org/topic_0622"},{"id":"T22","span":{"begin":813,"end":821},"obj":"http://edamontology.org/topic_1312"},{"id":"T23","span":{"begin":813,"end":821},"obj":"http://edamontology.org/topic_0111"},{"id":"T24","span":{"begin":813,"end":821},"obj":"http://edamontology.org/topic_0749"},{"id":"T25","span":{"begin":922,"end":936},"obj":"http://edamontology.org/topic_0659"},{"id":"T26","span":{"begin":1032,"end":1040},"obj":"http://edamontology.org/topic_1312"},{"id":"T27","span":{"begin":1032,"end":1040},"obj":"http://edamontology.org/topic_0111"},{"id":"T28","span":{"begin":1032,"end":1040},"obj":"http://edamontology.org/topic_0749"},{"id":"T29","span":{"begin":1054,"end":1061},"obj":"http://edamontology.org/topic_0622"},{"id":"T30","span":{"begin":1093,"end":1102},"obj":"http://edamontology.org/topic_0080"},{"id":"T31","span":{"begin":1093,"end":1102},"obj":"http://edamontology.org/topic_3168"},{"id":"T32","span":{"begin":1135,"end":1143},"obj":"http://edamontology.org/topic_3168"},{"id":"T33","span":{"begin":1135,"end":1143},"obj":"http://edamontology.org/topic_0080"},{"id":"T34","span":{"begin":1173,"end":1179},"obj":"http://edamontology.org/topic_0158"},{"id":"T35","span":{"begin":1189,"end":1197},"obj":"http://edamontology.org/topic_3168"},{"id":"T36","span":{"begin":1189,"end":1197},"obj":"http://edamontology.org/topic_0080"},{"id":"T37","span":{"begin":1189,"end":1204},"obj":"http://edamontology.org/topic_0160"},{"id":"T38","span":{"begin":1269,"end":1275},"obj":"http://edamontology.org/topic_0158"},{"id":"T39","span":{"begin":1290,"end":1295},"obj":"http://edamontology.org/topic_0158"},{"id":"T40","span":{"begin":1314,"end":1329},"obj":"http://edamontology.org/topic_0203"},{"id":"T41","span":{"begin":1314,"end":1329},"obj":"http://edamontology.org/topic_3308"},{"id":"T42","span":{"begin":1314,"end":1329},"obj":"http://edamontology.org/topic_3512"},{"id":"T43","span":{"begin":1314,"end":1329},"obj":"http://edamontology.org/topic_0110"},{"id":"T44","span":{"begin":1355,"end":1363},"obj":"http://edamontology.org/topic_0749"},{"id":"T45","span":{"begin":1486,"end":1494},"obj":"http://edamontology.org/topic_0080"},{"id":"T46","span":{"begin":1486,"end":1494},"obj":"http://edamontology.org/topic_3168"},{"id":"T47","span":{"begin":1527,"end":1542},"obj":"http://edamontology.org/topic_3512"},{"id":"T48","span":{"begin":1527,"end":1542},"obj":"http://edamontology.org/topic_0203"},{"id":"T49","span":{"begin":1527,"end":1542},"obj":"http://edamontology.org/topic_0110"},{"id":"T50","span":{"begin":1527,"end":1542},"obj":"http://edamontology.org/topic_3308"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"http://edamontology.org/operation_3642"},{"id":"T2","span":{"begin":593,"end":599},"obj":"http://edamontology.org/data_2048"},{"id":"T3","span":{"begin":852,"end":864},"obj":"http://edamontology.org/operation_0004"},{"id":"T4","span":{"begin":909,"end":919},"obj":"http://edamontology.org/data_0842"},{"id":"T5","span":{"begin":909,"end":919},"obj":"http://edamontology.org/data_2611"},{"id":"T6","span":{"begin":982,"end":992},"obj":"http://edamontology.org/format_1207"},{"id":"T7","span":{"begin":1093,"end":1102},"obj":"http://edamontology.org/operation_3218"},{"id":"T8","span":{"begin":1093,"end":1102},"obj":"http://edamontology.org/data_2044"},{"id":"T9","span":{"begin":1093,"end":1110},"obj":"http://edamontology.org/data_0865"},{"id":"T10","span":{"begin":1093,"end":1110},"obj":"http://edamontology.org/data_1413"},{"id":"T11","span":{"begin":1093,"end":1110},"obj":"http://edamontology.org/data_2161"},{"id":"T12","span":{"begin":1135,"end":1143},"obj":"http://edamontology.org/data_2044"},{"id":"T13","span":{"begin":1135,"end":1143},"obj":"http://edamontology.org/operation_3218"},{"id":"T14","span":{"begin":1135,"end":1151},"obj":"http://edamontology.org/data_1413"},{"id":"T15","span":{"begin":1135,"end":1151},"obj":"http://edamontology.org/data_2161"},{"id":"T16","span":{"begin":1135,"end":1151},"obj":"http://edamontology.org/data_0865"},{"id":"T17","span":{"begin":1161,"end":1172},"obj":"http://edamontology.org/operation_2423"},{"id":"T18","span":{"begin":1189,"end":1197},"obj":"http://edamontology.org/operation_3218"},{"id":"T19","span":{"begin":1189,"end":1197},"obj":"http://edamontology.org/data_2044"},{"id":"T20","span":{"begin":1486,"end":1494},"obj":"http://edamontology.org/operation_3218"},{"id":"T21","span":{"begin":1486,"end":1494},"obj":"http://edamontology.org/data_2044"},{"id":"T22","span":{"begin":1486,"end":1498},"obj":"http://edamontology.org/data_1279"},{"id":"T23","span":{"begin":1486,"end":1498},"obj":"http://edamontology.org/operation_2871"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos600-FMA","denotations":[{"id":"PD-FMA-PAE-B_T1","span":{"begin":93,"end":97},"obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"PD-FMA-PAE-B_T2","span":{"begin":134,"end":138},"obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"PD-FMA-PAE-B_T3","span":{"begin":249,"end":253},"obj":"http://purl.org/sig/ont/fma/fma67122"},{"id":"PD-FMA-PAE-B_T4","span":{"begin":422,"end":426},"obj":"http://purl.org/sig/ont/fma/fma84120"},{"id":"PD-FMA-PAE-B_T5","span":{"begin":571,"end":576},"obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"PD-FMA-PAE-B_T6","span":{"begin":656,"end":660},"obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"PD-FMA-PAE-B_T7","span":{"begin":706,"end":711},"obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"PD-FMA-PAE-B_T8","span":{"begin":759,"end":763},"obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"PD-FMA-PAE-B_T9","span":{"begin":780,"end":787},"obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"PD-FMA-PAE-B_T10","span":{"begin":933,"end":936},"obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"PD-FMA-PAE-B_T11","span":{"begin":982,"end":992},"obj":"http://purl.org/sig/ont/fma/fma82740"},{"id":"PD-FMA-PAE-B_T12","span":{"begin":1054,"end":1061},"obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"PD-FMA-PAE-B_T13","span":{"begin":1062,"end":1065},"obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"PD-FMA-PAE-B_T14","span":{"begin":1348,"end":1353},"obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"PD-FMA-PAE-B_T15","span":{"begin":1400,"end":1404},"obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"PD-FMA-PAE-B_T16","span":{"begin":1571,"end":1575},"obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"PD-FMA-PAE-B_T17","span":{"begin":1589,"end":1593},"obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"pubmed-enju-pas","denotations":[{"id":"EnjuParser_T0","span":{"begin":0,"end":15},"obj":"JJ"},{"id":"EnjuParser_T1","span":{"begin":16,"end":26},"obj":"NN"},{"id":"EnjuParser_T2","span":{"begin":27,"end":29},"obj":"IN"},{"id":"EnjuParser_T3","span":{"begin":30,"end":35},"obj":"JJ"},{"id":"EnjuParser_T4","span":{"begin":36,"end":52},"obj":"NN"},{"id":"EnjuParser_T5","span":{"begin":53,"end":59},"obj":"NN"},{"id":"EnjuParser_T6","span":{"begin":59,"end":60},"obj":"-COMMA-"},{"id":"EnjuParser_T7","span":{"begin":61,"end":80},"obj":"NN"},{"id":"EnjuParser_T8","span":{"begin":81,"end":82},"obj":"-LRB-"},{"id":"EnjuParser_T9","span":{"begin":82,"end":89},"obj":"NN"},{"id":"EnjuParser_T10","span":{"begin":90,"end":91},"obj":"CD"},{"id":"EnjuParser_T11","span":{"begin":91,"end":92},"obj":"-RRB-"},{"id":"EnjuParser_T12","span":{"begin":93,"end":97},"obj":"NN"},{"id":"EnjuParser_T13","span":{"begin":98,"end":104},"obj":"IN"},{"id":"EnjuParser_T14","span":{"begin":105,"end":120},"obj":"NN"},{"id":"EnjuParser_T15","span":{"begin":121,"end":123},"obj":"IN"},{"id":"EnjuParser_T16","span":{"begin":124,"end":127},"obj":"DT"},{"id":"EnjuParser_T17","span":{"begin":128,"end":133},"obj":"NN"},{"id":"EnjuParser_T18","span":{"begin":134,"end":138},"obj":"NN"},{"id":"EnjuParser_T19","span":{"begin":139,"end":143},"obj":"NN"},{"id":"EnjuParser_T20","span":{"begin":145,"end":147},"obj":"PRP"},{"id":"EnjuParser_T21","span":{"begin":148,"end":152},"obj":"VBP"},{"id":"EnjuParser_T22","span":{"begin":153,"end":163},"obj":"RB"},{"id":"EnjuParser_T23","span":{"begin":164,"end":169},"obj":"VBN"},{"id":"EnjuParser_T24","span":{"begin":170,"end":174},"obj":"IN"},{"id":"EnjuParser_T25","span":{"begin":175,"end":178},"obj":"DT"},{"id":"EnjuParser_T26","span":{"begin":179,"end":189},"obj":"NN"},{"id":"EnjuParser_T27","span":{"begin":190,"end":192},"obj":"IN"},{"id":"EnjuParser_T28","span":{"begin":193,"end":209},"obj":"NN"},{"id":"EnjuParser_T29","span":{"begin":210,"end":236},"obj":"NN"},{"id":"EnjuParser_T30","span":{"begin":237,"end":238},"obj":"-LRB-"},{"id":"EnjuParser_T31","span":{"begin":238,"end":245},"obj":"NN"},{"id":"EnjuParser_T32","span":{"begin":246,"end":247},"obj":"CD"},{"id":"EnjuParser_T33","span":{"begin":247,"end":248},"obj":"-RRB-"},{"id":"EnjuParser_T34","span":{"begin":249,"end":253},"obj":"NN"},{"id":"EnjuParser_T35","span":{"begin":254,"end":263},"obj":"VBZ"},{"id":"EnjuParser_T36","span":{"begin":264,"end":270},"obj":"IN"},{"id":"EnjuParser_T37","span":{"begin":271,"end":276},"obj":"NN"},{"id":"EnjuParser_T38","span":{"begin":277,"end":292},"obj":"NN"},{"id":"EnjuParser_T39","span":{"begin":293,"end":300},"obj":"VBN"},{"id":"EnjuParser_T40","span":{"begin":301,"end":305},"obj":"IN"},{"id":"EnjuParser_T41","span":{"begin":306,"end":314},"obj":"NN"},{"id":"EnjuParser_T42","span":{"begin":315,"end":324},"obj":"NN"},{"id":"EnjuParser_T43","span":{"begin":325,"end":326},"obj":"-LRB-"},{"id":"EnjuParser_T44","span":{"begin":326,"end":330},"obj":"NN"},{"id":"EnjuParser_T45","span":{"begin":330,"end":331},"obj":"-RRB-"},{"id":"EnjuParser_T46","span":{"begin":332,"end":335},"obj":"CC"},{"id":"EnjuParser_T47","span":{"begin":336,"end":340},"obj":"IN"},{"id":"EnjuParser_T48","span":{"begin":341,"end":356},"obj":"JJ"},{"id":"EnjuParser_T49","span":{"begin":357,"end":367},"obj":"NN"},{"id":"EnjuParser_T50","span":{"begin":368,"end":375},"obj":"VBZ"},{"id":"EnjuParser_T51","span":{"begin":376,"end":378},"obj":"IN"},{"id":"EnjuParser_T52","span":{"begin":379,"end":382},"obj":"DT"},{"id":"EnjuParser_T53","span":{"begin":383,"end":385},"obj":"NN"},{"id":"EnjuParser_T54","span":{"begin":386,"end":394},"obj":"NN"},{"id":"EnjuParser_T55","span":{"begin":395,"end":399},"obj":"WDT"},{"id":"EnjuParser_T56","span":{"begin":400,"end":406},"obj":"VBZ"},{"id":"EnjuParser_T57","span":{"begin":407,"end":418},"obj":"JJ"},{"id":"EnjuParser_T58","span":{"begin":419,"end":421},"obj":"IN"},{"id":"EnjuParser_T59","span":{"begin":422,"end":426},"obj":"NN"},{"id":"EnjuParser_T60","span":{"begin":427,"end":428},"obj":"NN"},{"id":"EnjuParser_T61","span":{"begin":429,"end":430},"obj":"-LRB-"},{"id":"EnjuParser_T62","span":{"begin":430,"end":432},"obj":"NN"},{"id":"EnjuParser_T63","span":{"begin":433,"end":442},"obj":"NNP"},{"id":"EnjuParser_T64","span":{"begin":443,"end":445},"obj":"FW"},{"id":"EnjuParser_T65","span":{"begin":446,"end":449},"obj":"FW"},{"id":"EnjuParser_T66","span":{"begin":449,"end":450},"obj":"-COMMA-"},{"id":"EnjuParser_T67","span":{"begin":451,"end":455},"obj":"NNP"},{"id":"EnjuParser_T68","span":{"begin":456,"end":461},"obj":"NNP"},{"id":"EnjuParser_T69","span":{"begin":461,"end":462},"obj":"-COMMA-"},{"id":"EnjuParser_T70","span":{"begin":462,"end":465},"obj":"CD"},{"id":"EnjuParser_T71","span":{"begin":465,"end":466},"obj":"-COMMA-"},{"id":"EnjuParser_T72","span":{"begin":467,"end":474},"obj":"CD"},{"id":"EnjuParser_T73","span":{"begin":474,"end":475},"obj":"-COMMA-"},{"id":"EnjuParser_T74","span":{"begin":476,"end":480},"obj":"CD"},{"id":"EnjuParser_T75","span":{"begin":480,"end":481},"obj":"-RRB-"},{"id":"EnjuParser_T76","span":{"begin":483,"end":486},"obj":"DT"},{"id":"EnjuParser_T77","span":{"begin":487,"end":497},"obj":"NN"},{"id":"EnjuParser_T78","span":{"begin":498,"end":500},"obj":"IN"},{"id":"EnjuParser_T79","span":{"begin":501,"end":508},"obj":"NN"},{"id":"EnjuParser_T80","span":{"begin":509,"end":510},"obj":"PRP"},{"id":"EnjuParser_T81","span":{"begin":511,"end":514},"obj":"MD"},{"id":"EnjuParser_T82","span":{"begin":515,"end":517},"obj":"VB"},{"id":"EnjuParser_T83","span":{"begin":518,"end":527},"obj":"JJ"},{"id":"EnjuParser_T84","span":{"begin":528,"end":531},"obj":"IN"},{"id":"EnjuParser_T85","span":{"begin":532,"end":535},"obj":"DT"},{"id":"EnjuParser_T86","span":{"begin":536,"end":546},"obj":"NN"},{"id":"EnjuParser_T87","span":{"begin":547,"end":549},"obj":"IN"},{"id":"EnjuParser_T88","span":{"begin":550,"end":559},"obj":"NN"},{"id":"EnjuParser_T89","span":{"begin":559,"end":560},"obj":"-LRB-"},{"id":"EnjuParser_T90","span":{"begin":560,"end":561},"obj":"NN"},{"id":"EnjuParser_T91","span":{"begin":561,"end":562},"obj":"-RRB-"},{"id":"EnjuParser_T92","span":{"begin":562,"end":564},"obj":"IN"},{"id":"EnjuParser_T93","span":{"begin":565,"end":570},"obj":"NN"},{"id":"EnjuParser_T94","span":{"begin":571,"end":576},"obj":"NNS"},{"id":"EnjuParser_T95","span":{"begin":578,"end":580},"obj":"IN"},{"id":"EnjuParser_T96","span":{"begin":581,"end":584},"obj":"DT"},{"id":"EnjuParser_T97","span":{"begin":585,"end":592},"obj":"JJ"},{"id":"EnjuParser_T98","span":{"begin":593,"end":599},"obj":"NN"},{"id":"EnjuParser_T99","span":{"begin":599,"end":600},"obj":"-COMMA-"},{"id":"EnjuParser_T100","span":{"begin":601,"end":603},"obj":"PRP"},{"id":"EnjuParser_T101","span":{"begin":604,"end":611},"obj":"VBD"},{"id":"EnjuParser_T102","span":{"begin":612,"end":615},"obj":"DT"},{"id":"EnjuParser_T103","span":{"begin":616,"end":631},"obj":"JJ"},{"id":"EnjuParser_T104","span":{"begin":632,"end":642},"obj":"NN"},{"id":"EnjuParser_T105","span":{"begin":643,"end":645},"obj":"IN"},{"id":"EnjuParser_T106","span":{"begin":646,"end":653},"obj":"NN"},{"id":"EnjuParser_T107","span":{"begin":654,"end":655},"obj":"CD"},{"id":"EnjuParser_T108","span":{"begin":656,"end":660},"obj":"NN"},{"id":"EnjuParser_T109","span":{"begin":661,"end":667},"obj":"IN"},{"id":"EnjuParser_T110","span":{"begin":668,"end":680},"obj":"JJ"},{"id":"EnjuParser_T111","span":{"begin":681,"end":696},"obj":"NN"},{"id":"EnjuParser_T112","span":{"begin":697,"end":699},"obj":"IN"},{"id":"EnjuParser_T113","span":{"begin":700,"end":705},"obj":"NN"},{"id":"EnjuParser_T114","span":{"begin":706,"end":711},"obj":"NNS"},{"id":"EnjuParser_T115","span":{"begin":713,"end":715},"obj":"TO"},{"id":"EnjuParser_T116","span":{"begin":716,"end":725},"obj":"VB"},{"id":"EnjuParser_T117","span":{"begin":726,"end":729},"obj":"DT"},{"id":"EnjuParser_T118","span":{"begin":730,"end":739},"obj":"JJ"},{"id":"EnjuParser_T119","span":{"begin":740,"end":745},"obj":"NN"},{"id":"EnjuParser_T120","span":{"begin":746,"end":748},"obj":"IN"},{"id":"EnjuParser_T121","span":{"begin":749,"end":756},"obj":"NN"},{"id":"EnjuParser_T122","span":{"begin":757,"end":758},"obj":"CD"},{"id":"EnjuParser_T123","span":{"begin":759,"end":763},"obj":"NN"},{"id":"EnjuParser_T124","span":{"begin":764,"end":774},"obj":"NN"},{"id":"EnjuParser_T125","span":{"begin":774,"end":775},"obj":"-COMMA-"},{"id":"EnjuParser_T126","span":{"begin":776,"end":779},"obj":"DT"},{"id":"EnjuParser_T127","span":{"begin":780,"end":787},"obj":"JJ"},{"id":"EnjuParser_T128","span":{"begin":788,"end":794},"obj":"NN"},{"id":"EnjuParser_T129","span":{"begin":795,"end":805},"obj":"VBG"},{"id":"EnjuParser_T130","span":{"begin":806,"end":809},"obj":"DT"},{"id":"EnjuParser_T131","span":{"begin":810,"end":812},"obj":"NN"},{"id":"EnjuParser_T132","span":{"begin":813,"end":821},"obj":"NN"},{"id":"EnjuParser_T133","span":{"begin":822,"end":824},"obj":"IN"},{"id":"EnjuParser_T134","span":{"begin":825,"end":832},"obj":"NN"},{"id":"EnjuParser_T135","span":{"begin":833,"end":834},"obj":"PRP"},{"id":"EnjuParser_T136","span":{"begin":835,"end":838},"obj":"VBD"},{"id":"EnjuParser_T137","span":{"begin":839,"end":847},"obj":"VBN"},{"id":"EnjuParser_T138","span":{"begin":848,"end":851},"obj":"CC"},{"id":"EnjuParser_T139","span":{"begin":852,"end":864},"obj":"RB"},{"id":"EnjuParser_T140","span":{"begin":865,"end":878},"obj":"VBN"},{"id":"EnjuParser_T141","span":{"begin":880,"end":885},"obj":"VBG"},{"id":"EnjuParser_T142","span":{"begin":886,"end":887},"obj":"DT"},{"id":"EnjuParser_T143","span":{"begin":888,"end":898},"obj":"NN"},{"id":"EnjuParser_T144","span":{"begin":899,"end":904},"obj":"NN"},{"id":"EnjuParser_T145","span":{"begin":904,"end":905},"obj":"-COMMA-"},{"id":"EnjuParser_T146","span":{"begin":906,"end":908},"obj":"PRP"},{"id":"EnjuParser_T147","span":{"begin":909,"end":919},"obj":"VBD"},{"id":"EnjuParser_T148","span":{"begin":920,"end":921},"obj":"DT"},{"id":"EnjuParser_T149","span":{"begin":922,"end":932},"obj":"JJ"},{"id":"EnjuParser_T150","span":{"begin":933,"end":936},"obj":"NN"},{"id":"EnjuParser_T151","span":{"begin":937,"end":944},"obj":"NN"},{"id":"EnjuParser_T152","span":{"begin":945,"end":949},"obj":"WDT"},{"id":"EnjuParser_T153","span":{"begin":950,"end":957},"obj":"VBZ"},{"id":"EnjuParser_T154","span":{"begin":958,"end":960},"obj":"DT"},{"id":"EnjuParser_T155","span":{"begin":961,"end":969},"obj":"NN"},{"id":"EnjuParser_T156","span":{"begin":969,"end":970},"obj":"-COMMA-"},{"id":"EnjuParser_T157","span":{"begin":971,"end":978},"obj":"JJ"},{"id":"EnjuParser_T158","span":{"begin":979,"end":981},"obj":"IN"},{"id":"EnjuParser_T159","span":{"begin":982,"end":992},"obj":"NN"},{"id":"EnjuParser_T160","span":{"begin":993,"end":999},"obj":"NN"},{"id":"EnjuParser_T161","span":{"begin":1000,"end":1001},"obj":"-LRB-"},{"id":"EnjuParser_T162","span":{"begin":1001,"end":1003},"obj":"NN"},{"id":"EnjuParser_T163","span":{"begin":1003,"end":1004},"obj":"-RRB-"},{"id":"EnjuParser_T164","span":{"begin":1005,"end":1009},"obj":"CD"},{"id":"EnjuParser_T165","span":{"begin":1010,"end":1012},"obj":"TO"},{"id":"EnjuParser_T166","span":{"begin":1013,"end":1017},"obj":"CD"},{"id":"EnjuParser_T167","span":{"begin":1018,"end":1024},"obj":"IN"},{"id":"EnjuParser_T168","span":{"begin":1025,"end":1028},"obj":"DT"},{"id":"EnjuParser_T169","span":{"begin":1029,"end":1031},"obj":"NN"},{"id":"EnjuParser_T170","span":{"begin":1032,"end":1040},"obj":"NN"},{"id":"EnjuParser_T171","span":{"begin":1041,"end":1043},"obj":"IN"},{"id":"EnjuParser_T172","span":{"begin":1044,"end":1051},"obj":"NN"},{"id":"EnjuParser_T173","span":{"begin":1052,"end":1053},"obj":"CD"},{"id":"EnjuParser_T174","span":{"begin":1054,"end":1061},"obj":"JJ"},{"id":"EnjuParser_T175","span":{"begin":1062,"end":1065},"obj":"NN"},{"id":"EnjuParser_T176","span":{"begin":1067,"end":1071},"obj":"DT"},{"id":"EnjuParser_T177","span":{"begin":1072,"end":1079},"obj":"NN"},{"id":"EnjuParser_T178","span":{"begin":1080,"end":1088},"obj":"VBZ"},{"id":"EnjuParser_T179","span":{"begin":1089,"end":1092},"obj":"CD"},{"id":"EnjuParser_T180","span":{"begin":1093,"end":1102},"obj":"NNS"},{"id":"EnjuParser_T181","span":{"begin":1103,"end":1110},"obj":"JJ"},{"id":"EnjuParser_T182","span":{"begin":1111,"end":1113},"obj":"TO"},{"id":"EnjuParser_T183","span":{"begin":1114,"end":1117},"obj":"NN"},{"id":"EnjuParser_T184","span":{"begin":1118,"end":1119},"obj":"-LRB-"},{"id":"EnjuParser_T185","span":{"begin":1119,"end":1125},"obj":"NN"},{"id":"EnjuParser_T186","span":{"begin":1125,"end":1126},"obj":"-RRB-"},{"id":"EnjuParser_T187","span":{"begin":1127,"end":1130},"obj":"CC"},{"id":"EnjuParser_T188","span":{"begin":1131,"end":1134},"obj":"CD"},{"id":"EnjuParser_T189","span":{"begin":1135,"end":1143},"obj":"NN"},{"id":"EnjuParser_T190","span":{"begin":1144,"end":1151},"obj":"JJ"},{"id":"EnjuParser_T191","span":{"begin":1152,"end":1154},"obj":"TO"},{"id":"EnjuParser_T192","span":{"begin":1155,"end":1160},"obj":"NN"},{"id":"EnjuParser_T193","span":{"begin":1161,"end":1172},"obj":"NN"},{"id":"EnjuParser_T194","span":{"begin":1173,"end":1179},"obj":"NNS"},{"id":"EnjuParser_T195","span":{"begin":1180,"end":1181},"obj":"-LRB-"},{"id":"EnjuParser_T196","span":{"begin":1181,"end":1188},"obj":"NN"},{"id":"EnjuParser_T197","span":{"begin":1189,"end":1197},"obj":"NN"},{"id":"EnjuParser_T198","span":{"begin":1197,"end":1198},"obj":"-RRB-"},{"id":"EnjuParser_T199","span":{"begin":1200,"end":1213},"obj":"JJ"},{"id":"EnjuParser_T200","span":{"begin":1214,"end":1225},"obj":"NN"},{"id":"EnjuParser_T201","span":{"begin":1226,"end":1228},"obj":"IN"},{"id":"EnjuParser_T202","span":{"begin":1229,"end":1232},"obj":"NN"},{"id":"EnjuParser_T203","span":{"begin":1233,"end":1236},"obj":"CC"},{"id":"EnjuParser_T204","span":{"begin":1237,"end":1242},"obj":"NN"},{"id":"EnjuParser_T205","span":{"begin":1243,"end":1248},"obj":"NNS"},{"id":"EnjuParser_T206","span":{"begin":1249,"end":1255},"obj":"VBD"},{"id":"EnjuParser_T207","span":{"begin":1256,"end":1260},"obj":"IN"},{"id":"EnjuParser_T208","span":{"begin":1261,"end":1264},"obj":"CD"},{"id":"EnjuParser_T209","span":{"begin":1265,"end":1268},"obj":"NN"},{"id":"EnjuParser_T210","span":{"begin":1269,"end":1275},"obj":"NNS"},{"id":"EnjuParser_T211","span":{"begin":1276,"end":1279},"obj":"CC"},{"id":"EnjuParser_T212","span":{"begin":1280,"end":1283},"obj":"CD"},{"id":"EnjuParser_T213","span":{"begin":1284,"end":1289},"obj":"NN"},{"id":"EnjuParser_T214","span":{"begin":1290,"end":1295},"obj":"NN"},{"id":"EnjuParser_T215","span":{"begin":1296,"end":1299},"obj":"VBP"},{"id":"EnjuParser_T216","span":{"begin":1300,"end":1309},"obj":"JJ"},{"id":"EnjuParser_T217","span":{"begin":1310,"end":1313},"obj":"IN"},{"id":"EnjuParser_T218","span":{"begin":1314,"end":1329},"obj":"JJ"},{"id":"EnjuParser_T219","span":{"begin":1330,"end":1338},"obj":"NN"},{"id":"EnjuParser_T220","span":{"begin":1339,"end":1341},"obj":"IN"},{"id":"EnjuParser_T221","span":{"begin":1342,"end":1347},"obj":"NN"},{"id":"EnjuParser_T222","span":{"begin":1348,"end":1353},"obj":"NNS"},{"id":"EnjuParser_T223","span":{"begin":1355,"end":1363},"obj":"NN"},{"id":"EnjuParser_T224","span":{"begin":1364,"end":1372},"obj":"NN"},{"id":"EnjuParser_T225","span":{"begin":1373,"end":1375},"obj":"VBZ"},{"id":"EnjuParser_T226","span":{"begin":1376,"end":1386},"obj":"VBN"},{"id":"EnjuParser_T227","span":{"begin":1387,"end":1393},"obj":"IN"},{"id":"EnjuParser_T228","span":{"begin":1394,"end":1399},"obj":"NN"},{"id":"EnjuParser_T229","span":{"begin":1400,"end":1404},"obj":"NN"},{"id":"EnjuParser_T230","span":{"begin":1405,"end":1420},"obj":"NN"},{"id":"EnjuParser_T231","span":{"begin":1421,"end":1428},"obj":"VBN"},{"id":"EnjuParser_T232","span":{"begin":1429,"end":1433},"obj":"IN"},{"id":"EnjuParser_T233","span":{"begin":1434,"end":1438},"obj":"NN"},{"id":"EnjuParser_T234","span":{"begin":1440,"end":1445},"obj":"DT"},{"id":"EnjuParser_T235","span":{"begin":1446,"end":1453},"obj":"NNS"},{"id":"EnjuParser_T236","span":{"begin":1454,"end":1461},"obj":"VBP"},{"id":"EnjuParser_T237","span":{"begin":1462,"end":1466},"obj":"IN"},{"id":"EnjuParser_T238","span":{"begin":1467,"end":1473},"obj":"NN"},{"id":"EnjuParser_T239","span":{"begin":1474,"end":1477},"obj":"CC"},{"id":"EnjuParser_T240","span":{"begin":1478,"end":1485},"obj":"NN"},{"id":"EnjuParser_T241","span":{"begin":1486,"end":1494},"obj":"NN"},{"id":"EnjuParser_T242","span":{"begin":1495,"end":1498},"obj":"MD"},{"id":"EnjuParser_T243","span":{"begin":1499,"end":1503},"obj":"VB"},{"id":"EnjuParser_T244","span":{"begin":1504,"end":1505},"obj":"DT"},{"id":"EnjuParser_T245","span":{"begin":1506,"end":1514},"obj":"JJ"},{"id":"EnjuParser_T246","span":{"begin":1515,"end":1519},"obj":"NN"},{"id":"EnjuParser_T247","span":{"begin":1520,"end":1522},"obj":"IN"},{"id":"EnjuParser_T248","span":{"begin":1523,"end":1526},"obj":"DT"},{"id":"EnjuParser_T249","span":{"begin":1527,"end":1542},"obj":"JJ"},{"id":"EnjuParser_T250","span":{"begin":1543,"end":1553},"obj":"NN"},{"id":"EnjuParser_T251","span":{"begin":1554,"end":1556},"obj":"IN"},{"id":"EnjuParser_T252","span":{"begin":1557,"end":1560},"obj":"DT"},{"id":"EnjuParser_T253","span":{"begin":1561,"end":1568},"obj":"NN"},{"id":"EnjuParser_T254","span":{"begin":1569,"end":1570},"obj":"CD"},{"id":"EnjuParser_T255","span":{"begin":1571,"end":1575},"obj":"NN"},{"id":"EnjuParser_T256","span":{"begin":1576,"end":1582},"obj":"IN"},{"id":"EnjuParser_T257","span":{"begin":1583,"end":1588},"obj":"NN"},{"id":"EnjuParser_T258","span":{"begin":1589,"end":1593},"obj":"NN"},{"id":"EnjuParser_T259","span":{"begin":1594,"end":1609},"obj":"NN"}],"relations":[{"id":"EnjuParser_R0","pred":"arg1Of","subj":"EnjuParser_T1","obj":"EnjuParser_T0"},{"id":"EnjuParser_R1","pred":"arg1Of","subj":"EnjuParser_T1","obj":"EnjuParser_T2"},{"id":"EnjuParser_R2","pred":"arg2Of","subj":"EnjuParser_T12","obj":"EnjuParser_T2"},{"id":"EnjuParser_R3","pred":"arg1Of","subj":"EnjuParser_T5","obj":"EnjuParser_T3"},{"id":"EnjuParser_R4","pred":"arg1Of","subj":"EnjuParser_T5","obj":"EnjuParser_T4"},{"id":"EnjuParser_R5","pred":"arg1Of","subj":"EnjuParser_T12","obj":"EnjuParser_T5"},{"id":"EnjuParser_R6","pred":"arg1Of","subj":"EnjuParser_T5","obj":"EnjuParser_T6"},{"id":"EnjuParser_R7","pred":"arg2Of","subj":"EnjuParser_T7","obj":"EnjuParser_T6"},{"id":"EnjuParser_R8","pred":"arg1Of","subj":"EnjuParser_T7","obj":"EnjuParser_T8"},{"id":"EnjuParser_R9","pred":"arg2Of","subj":"EnjuParser_T9","obj":"EnjuParser_T8"},{"id":"EnjuParser_R10","pred":"arg3Of","subj":"EnjuParser_T11","obj":"EnjuParser_T8"},{"id":"EnjuParser_R11","pred":"arg1Of","subj":"EnjuParser_T9","obj":"EnjuParser_T10"},{"id":"EnjuParser_R12","pred":"arg1Of","subj":"EnjuParser_T12","obj":"EnjuParser_T13"},{"id":"EnjuParser_R13","pred":"arg2Of","subj":"EnjuParser_T14","obj":"EnjuParser_T13"},{"id":"EnjuParser_R14","pred":"arg1Of","subj":"EnjuParser_T14","obj":"EnjuParser_T15"},{"id":"EnjuParser_R15","pred":"arg2Of","subj":"EnjuParser_T19","obj":"EnjuParser_T15"},{"id":"EnjuParser_R16","pred":"arg1Of","subj":"EnjuParser_T19","obj":"EnjuParser_T16"},{"id":"EnjuParser_R17","pred":"arg1Of","subj":"EnjuParser_T19","obj":"EnjuParser_T17"},{"id":"EnjuParser_R18","pred":"arg1Of","subj":"EnjuParser_T19","obj":"EnjuParser_T18"},{"id":"EnjuParser_R19","pred":"arg1Of","subj":"EnjuParser_T20","obj":"EnjuParser_T21"},{"id":"EnjuParser_R20","pred":"arg2Of","subj":"EnjuParser_T23","obj":"EnjuParser_T21"},{"id":"EnjuParser_R21","pred":"arg1Of","subj":"EnjuParser_T23","obj":"EnjuParser_T22"},{"id":"EnjuParser_R22","pred":"arg1Of","subj":"EnjuParser_T20","obj":"EnjuParser_T23"},{"id":"EnjuParser_R23","pred":"arg2Of","subj":"EnjuParser_T46","obj":"EnjuParser_T23"},{"id":"EnjuParser_R24","pred":"arg1Of","subj":"EnjuParser_T35","obj":"EnjuParser_T24"},{"id":"EnjuParser_R25","pred":"arg1Of","subj":"EnjuParser_T26","obj":"EnjuParser_T25"},{"id":"EnjuParser_R26","pred":"arg1Of","subj":"EnjuParser_T26","obj":"EnjuParser_T27"},{"id":"EnjuParser_R27","pred":"arg2Of","subj":"EnjuParser_T34","obj":"EnjuParser_T27"},{"id":"EnjuParser_R28","pred":"arg1Of","subj":"EnjuParser_T29","obj":"EnjuParser_T28"},{"id":"EnjuParser_R29","pred":"arg1Of","subj":"EnjuParser_T34","obj":"EnjuParser_T29"},{"id":"EnjuParser_R30","pred":"arg1Of","subj":"EnjuParser_T29","obj":"EnjuParser_T30"},{"id":"EnjuParser_R31","pred":"arg2Of","subj":"EnjuParser_T31","obj":"EnjuParser_T30"},{"id":"EnjuParser_R32","pred":"arg3Of","subj":"EnjuParser_T33","obj":"EnjuParser_T30"},{"id":"EnjuParser_R33","pred":"arg1Of","subj":"EnjuParser_T31","obj":"EnjuParser_T32"},{"id":"EnjuParser_R34","pred":"arg1Of","subj":"EnjuParser_T26","obj":"EnjuParser_T35"},{"id":"EnjuParser_R35","pred":"arg1Of","subj":"EnjuParser_T35","obj":"EnjuParser_T36"},{"id":"EnjuParser_R36","pred":"arg2Of","subj":"EnjuParser_T38","obj":"EnjuParser_T36"},{"id":"EnjuParser_R37","pred":"arg1Of","subj":"EnjuParser_T38","obj":"EnjuParser_T37"},{"id":"EnjuParser_R38","pred":"arg2Of","subj":"EnjuParser_T38","obj":"EnjuParser_T39"},{"id":"EnjuParser_R39","pred":"arg1Of","subj":"EnjuParser_T39","obj":"EnjuParser_T40"},{"id":"EnjuParser_R40","pred":"arg2Of","subj":"EnjuParser_T42","obj":"EnjuParser_T40"},{"id":"EnjuParser_R41","pred":"arg1Of","subj":"EnjuParser_T42","obj":"EnjuParser_T41"},{"id":"EnjuParser_R42","pred":"arg1Of","subj":"EnjuParser_T42","obj":"EnjuParser_T43"},{"id":"EnjuParser_R43","pred":"arg2Of","subj":"EnjuParser_T44","obj":"EnjuParser_T43"},{"id":"EnjuParser_R44","pred":"arg3Of","subj":"EnjuParser_T45","obj":"EnjuParser_T43"},{"id":"EnjuParser_R45","pred":"arg1Of","subj":"EnjuParser_T35","obj":"EnjuParser_T46"},{"id":"EnjuParser_R46","pred":"arg2Of","subj":"EnjuParser_T47","obj":"EnjuParser_T46"},{"id":"EnjuParser_R47","pred":"arg2Of","subj":"EnjuParser_T50","obj":"EnjuParser_T47"},{"id":"EnjuParser_R48","pred":"arg1Of","subj":"EnjuParser_T49","obj":"EnjuParser_T48"},{"id":"EnjuParser_R49","pred":"arg1Of","subj":"EnjuParser_T49","obj":"EnjuParser_T50"},{"id":"EnjuParser_R50","pred":"arg1Of","subj":"EnjuParser_T50","obj":"EnjuParser_T51"},{"id":"EnjuParser_R51","pred":"arg2Of","subj":"EnjuParser_T54","obj":"EnjuParser_T51"},{"id":"EnjuParser_R52","pred":"arg1Of","subj":"EnjuParser_T54","obj":"EnjuParser_T52"},{"id":"EnjuParser_R53","pred":"arg1Of","subj":"EnjuParser_T54","obj":"EnjuParser_T53"},{"id":"EnjuParser_R54","pred":"arg1Of","subj":"EnjuParser_T54","obj":"EnjuParser_T55"},{"id":"EnjuParser_R55","pred":"arg1Of","subj":"EnjuParser_T54","obj":"EnjuParser_T56"},{"id":"EnjuParser_R56","pred":"arg2Of","subj":"EnjuParser_T60","obj":"EnjuParser_T56"},{"id":"EnjuParser_R57","pred":"arg1Of","subj":"EnjuParser_T56","obj":"EnjuParser_T57"},{"id":"EnjuParser_R58","pred":"arg1Of","subj":"EnjuParser_T60","obj":"EnjuParser_T58"},{"id":"EnjuParser_R59","pred":"arg1Of","subj":"EnjuParser_T60","obj":"EnjuParser_T59"},{"id":"EnjuParser_R60","pred":"arg1Of","subj":"EnjuParser_T60","obj":"EnjuParser_T61"},{"id":"EnjuParser_R61","pred":"arg2Of","subj":"EnjuParser_T63","obj":"EnjuParser_T61"},{"id":"EnjuParser_R62","pred":"arg3Of","subj":"EnjuParser_T75","obj":"EnjuParser_T61"},{"id":"EnjuParser_R63","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T62"},{"id":"EnjuParser_R64","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T64"},{"id":"EnjuParser_R65","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T65"},{"id":"EnjuParser_R66","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T66"},{"id":"EnjuParser_R67","pred":"arg2Of","subj":"EnjuParser_T68","obj":"EnjuParser_T66"},{"id":"EnjuParser_R68","pred":"arg1Of","subj":"EnjuParser_T68","obj":"EnjuParser_T67"},{"id":"EnjuParser_R69","pred":"arg1Of","subj":"EnjuParser_T68","obj":"EnjuParser_T69"},{"id":"EnjuParser_R70","pred":"arg2Of","subj":"EnjuParser_T70","obj":"EnjuParser_T69"},{"id":"EnjuParser_R71","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T71"},{"id":"EnjuParser_R72","pred":"arg2Of","subj":"EnjuParser_T72","obj":"EnjuParser_T71"},{"id":"EnjuParser_R73","pred":"arg1Of","subj":"EnjuParser_T63","obj":"EnjuParser_T73"},{"id":"EnjuParser_R74","pred":"arg2Of","subj":"EnjuParser_T74","obj":"EnjuParser_T73"},{"id":"EnjuParser_R75","pred":"arg1Of","subj":"EnjuParser_T77","obj":"EnjuParser_T76"},{"id":"EnjuParser_R76","pred":"arg1Of","subj":"EnjuParser_T77","obj":"EnjuParser_T78"},{"id":"EnjuParser_R77","pred":"arg2Of","subj":"EnjuParser_T79","obj":"EnjuParser_T78"},{"id":"EnjuParser_R78","pred":"arg1Of","subj":"EnjuParser_T80","obj":"EnjuParser_T81"},{"id":"EnjuParser_R79","pred":"arg2Of","subj":"EnjuParser_T82","obj":"EnjuParser_T81"},{"id":"EnjuParser_R80","pred":"modOf","subj":"EnjuParser_T77","obj":"EnjuParser_T81"},{"id":"EnjuParser_R81","pred":"arg1Of","subj":"EnjuParser_T80","obj":"EnjuParser_T82"},{"id":"EnjuParser_R82","pred":"arg2Of","subj":"EnjuParser_T83","obj":"EnjuParser_T82"},{"id":"EnjuParser_R83","pred":"arg1Of","subj":"EnjuParser_T80","obj":"EnjuParser_T83"},{"id":"EnjuParser_R84","pred":"arg1Of","subj":"EnjuParser_T83","obj":"EnjuParser_T84"},{"id":"EnjuParser_R85","pred":"arg2Of","subj":"EnjuParser_T86","obj":"EnjuParser_T84"},{"id":"EnjuParser_R86","pred":"arg1Of","subj":"EnjuParser_T86","obj":"EnjuParser_T85"},{"id":"EnjuParser_R87","pred":"arg1Of","subj":"EnjuParser_T86","obj":"EnjuParser_T87"},{"id":"EnjuParser_R88","pred":"arg2Of","subj":"EnjuParser_T88","obj":"EnjuParser_T87"},{"id":"EnjuParser_R89","pred":"arg1Of","subj":"EnjuParser_T88","obj":"EnjuParser_T89"},{"id":"EnjuParser_R90","pred":"arg2Of","subj":"EnjuParser_T90","obj":"EnjuParser_T89"},{"id":"EnjuParser_R91","pred":"arg3Of","subj":"EnjuParser_T91","obj":"EnjuParser_T89"},{"id":"EnjuParser_R92","pred":"arg1Of","subj":"EnjuParser_T86","obj":"EnjuParser_T92"},{"id":"EnjuParser_R93","pred":"arg2Of","subj":"EnjuParser_T94","obj":"EnjuParser_T92"},{"id":"EnjuParser_R94","pred":"arg1Of","subj":"EnjuParser_T94","obj":"EnjuParser_T93"},{"id":"EnjuParser_R95","pred":"arg1Of","subj":"EnjuParser_T101","obj":"EnjuParser_T95"},{"id":"EnjuParser_R96","pred":"arg2Of","subj":"EnjuParser_T98","obj":"EnjuParser_T95"},{"id":"EnjuParser_R97","pred":"arg1Of","subj":"EnjuParser_T98","obj":"EnjuParser_T96"},{"id":"EnjuParser_R98","pred":"arg1Of","subj":"EnjuParser_T98","obj":"EnjuParser_T97"},{"id":"EnjuParser_R99","pred":"arg1Of","subj":"EnjuParser_T101","obj":"EnjuParser_T99"},{"id":"EnjuParser_R100","pred":"arg1Of","subj":"EnjuParser_T100","obj":"EnjuParser_T101"},{"id":"EnjuParser_R101","pred":"arg2Of","subj":"EnjuParser_T104","obj":"EnjuParser_T101"},{"id":"EnjuParser_R102","pred":"arg1Of","subj":"EnjuParser_T104","obj":"EnjuParser_T102"},{"id":"EnjuParser_R103","pred":"arg1Of","subj":"EnjuParser_T104","obj":"EnjuParser_T103"},{"id":"EnjuParser_R104","pred":"arg1Of","subj":"EnjuParser_T104","obj":"EnjuParser_T105"},{"id":"EnjuParser_R105","pred":"arg2Of","subj":"EnjuParser_T108","obj":"EnjuParser_T105"},{"id":"EnjuParser_R106","pred":"arg1Of","subj":"EnjuParser_T108","obj":"EnjuParser_T106"},{"id":"EnjuParser_R107","pred":"arg1Of","subj":"EnjuParser_T108","obj":"EnjuParser_T107"},{"id":"EnjuParser_R108","pred":"arg1Of","subj":"EnjuParser_T101","obj":"EnjuParser_T109"},{"id":"EnjuParser_R109","pred":"arg2Of","subj":"EnjuParser_T111","obj":"EnjuParser_T109"},{"id":"EnjuParser_R110","pred":"arg1Of","subj":"EnjuParser_T111","obj":"EnjuParser_T110"},{"id":"EnjuParser_R111","pred":"arg1Of","subj":"EnjuParser_T111","obj":"EnjuParser_T112"},{"id":"EnjuParser_R112","pred":"arg2Of","subj":"EnjuParser_T114","obj":"EnjuParser_T112"},{"id":"EnjuParser_R113","pred":"arg1Of","subj":"EnjuParser_T114","obj":"EnjuParser_T113"},{"id":"EnjuParser_R114","pred":"arg1Of","subj":"EnjuParser_T116","obj":"EnjuParser_T115"},{"id":"EnjuParser_R115","pred":"modOf","subj":"EnjuParser_T138","obj":"EnjuParser_T115"},{"id":"EnjuParser_R116","pred":"arg2Of","subj":"EnjuParser_T119","obj":"EnjuParser_T116"},{"id":"EnjuParser_R117","pred":"arg1Of","subj":"EnjuParser_T119","obj":"EnjuParser_T117"},{"id":"EnjuParser_R118","pred":"arg1Of","subj":"EnjuParser_T119","obj":"EnjuParser_T118"},{"id":"EnjuParser_R119","pred":"arg1Of","subj":"EnjuParser_T119","obj":"EnjuParser_T120"},{"id":"EnjuParser_R120","pred":"arg2Of","subj":"EnjuParser_T124","obj":"EnjuParser_T120"},{"id":"EnjuParser_R121","pred":"arg1Of","subj":"EnjuParser_T124","obj":"EnjuParser_T121"},{"id":"EnjuParser_R122","pred":"arg1Of","subj":"EnjuParser_T124","obj":"EnjuParser_T122"},{"id":"EnjuParser_R123","pred":"arg1Of","subj":"EnjuParser_T124","obj":"EnjuParser_T123"},{"id":"EnjuParser_R124","pred":"arg1Of","subj":"EnjuParser_T138","obj":"EnjuParser_T125"},{"id":"EnjuParser_R125","pred":"arg1Of","subj":"EnjuParser_T128","obj":"EnjuParser_T126"},{"id":"EnjuParser_R126","pred":"arg1Of","subj":"EnjuParser_T128","obj":"EnjuParser_T127"},{"id":"EnjuParser_R127","pred":"arg1Of","subj":"EnjuParser_T128","obj":"EnjuParser_T129"},{"id":"EnjuParser_R128","pred":"arg2Of","subj":"EnjuParser_T132","obj":"EnjuParser_T129"},{"id":"EnjuParser_R129","pred":"arg1Of","subj":"EnjuParser_T132","obj":"EnjuParser_T130"},{"id":"EnjuParser_R130","pred":"arg1Of","subj":"EnjuParser_T132","obj":"EnjuParser_T131"},{"id":"EnjuParser_R131","pred":"arg1Of","subj":"EnjuParser_T132","obj":"EnjuParser_T133"},{"id":"EnjuParser_R132","pred":"arg2Of","subj":"EnjuParser_T134","obj":"EnjuParser_T133"},{"id":"EnjuParser_R133","pred":"arg1Of","subj":"EnjuParser_T132","obj":"EnjuParser_T135"},{"id":"EnjuParser_R134","pred":"arg1Of","subj":"EnjuParser_T128","obj":"EnjuParser_T136"},{"id":"EnjuParser_R135","pred":"arg2Of","subj":"EnjuParser_T138","obj":"EnjuParser_T136"},{"id":"EnjuParser_R136","pred":"arg2Of","subj":"EnjuParser_T128","obj":"EnjuParser_T137"},{"id":"EnjuParser_R137","pred":"arg1Of","subj":"EnjuParser_T137","obj":"EnjuParser_T138"},{"id":"EnjuParser_R138","pred":"arg2Of","subj":"EnjuParser_T140","obj":"EnjuParser_T138"},{"id":"EnjuParser_R139","pred":"arg1Of","subj":"EnjuParser_T140","obj":"EnjuParser_T139"},{"id":"EnjuParser_R140","pred":"arg2Of","subj":"EnjuParser_T128","obj":"EnjuParser_T140"},{"id":"EnjuParser_R141","pred":"arg1Of","subj":"EnjuParser_T146","obj":"EnjuParser_T141"},{"id":"EnjuParser_R142","pred":"arg2Of","subj":"EnjuParser_T144","obj":"EnjuParser_T141"},{"id":"EnjuParser_R143","pred":"modOf","subj":"EnjuParser_T147","obj":"EnjuParser_T141"},{"id":"EnjuParser_R144","pred":"arg1Of","subj":"EnjuParser_T144","obj":"EnjuParser_T142"},{"id":"EnjuParser_R145","pred":"arg1Of","subj":"EnjuParser_T144","obj":"EnjuParser_T143"},{"id":"EnjuParser_R146","pred":"arg1Of","subj":"EnjuParser_T147","obj":"EnjuParser_T145"},{"id":"EnjuParser_R147","pred":"arg1Of","subj":"EnjuParser_T146","obj":"EnjuParser_T147"},{"id":"EnjuParser_R148","pred":"arg2Of","subj":"EnjuParser_T151","obj":"EnjuParser_T147"},{"id":"EnjuParser_R149","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T148"},{"id":"EnjuParser_R150","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T149"},{"id":"EnjuParser_R151","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T150"},{"id":"EnjuParser_R152","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T152"},{"id":"EnjuParser_R153","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T153"},{"id":"EnjuParser_R154","pred":"arg2Of","subj":"EnjuParser_T155","obj":"EnjuParser_T153"},{"id":"EnjuParser_R155","pred":"arg1Of","subj":"EnjuParser_T155","obj":"EnjuParser_T154"},{"id":"EnjuParser_R156","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T156"},{"id":"EnjuParser_R157","pred":"arg1Of","subj":"EnjuParser_T151","obj":"EnjuParser_T157"},{"id":"EnjuParser_R158","pred":"arg1Of","subj":"EnjuParser_T157","obj":"EnjuParser_T158"},{"id":"EnjuParser_R159","pred":"arg2Of","subj":"EnjuParser_T160","obj":"EnjuParser_T158"},{"id":"EnjuParser_R160","pred":"arg1Of","subj":"EnjuParser_T160","obj":"EnjuParser_T159"},{"id":"EnjuParser_R161","pred":"arg1Of","subj":"EnjuParser_T160","obj":"EnjuParser_T161"},{"id":"EnjuParser_R162","pred":"arg2Of","subj":"EnjuParser_T162","obj":"EnjuParser_T161"},{"id":"EnjuParser_R163","pred":"arg3Of","subj":"EnjuParser_T163","obj":"EnjuParser_T161"},{"id":"EnjuParser_R164","pred":"arg1Of","subj":"EnjuParser_T160","obj":"EnjuParser_T164"},{"id":"EnjuParser_R165","pred":"arg1Of","subj":"EnjuParser_T164","obj":"EnjuParser_T165"},{"id":"EnjuParser_R166","pred":"arg2Of","subj":"EnjuParser_T166","obj":"EnjuParser_T165"},{"id":"EnjuParser_R167","pred":"arg1Of","subj":"EnjuParser_T160","obj":"EnjuParser_T167"},{"id":"EnjuParser_R168","pred":"arg2Of","subj":"EnjuParser_T170","obj":"EnjuParser_T167"},{"id":"EnjuParser_R169","pred":"arg1Of","subj":"EnjuParser_T170","obj":"EnjuParser_T168"},{"id":"EnjuParser_R170","pred":"arg1Of","subj":"EnjuParser_T170","obj":"EnjuParser_T169"},{"id":"EnjuParser_R171","pred":"arg1Of","subj":"EnjuParser_T170","obj":"EnjuParser_T171"},{"id":"EnjuParser_R172","pred":"arg2Of","subj":"EnjuParser_T175","obj":"EnjuParser_T171"},{"id":"EnjuParser_R173","pred":"arg1Of","subj":"EnjuParser_T175","obj":"EnjuParser_T172"},{"id":"EnjuParser_R174","pred":"arg1Of","subj":"EnjuParser_T175","obj":"EnjuParser_T173"},{"id":"EnjuParser_R175","pred":"arg1Of","subj":"EnjuParser_T175","obj":"EnjuParser_T174"},{"id":"EnjuParser_R176","pred":"arg1Of","subj":"EnjuParser_T177","obj":"EnjuParser_T176"},{"id":"EnjuParser_R177","pred":"arg1Of","subj":"EnjuParser_T177","obj":"EnjuParser_T178"},{"id":"EnjuParser_R178","pred":"arg2Of","subj":"EnjuParser_T180","obj":"EnjuParser_T178"},{"id":"EnjuParser_R179","pred":"arg1Of","subj":"EnjuParser_T180","obj":"EnjuParser_T179"},{"id":"EnjuParser_R180","pred":"arg1Of","subj":"EnjuParser_T180","obj":"EnjuParser_T181"},{"id":"EnjuParser_R181","pred":"arg1Of","subj":"EnjuParser_T181","obj":"EnjuParser_T182"},{"id":"EnjuParser_R182","pred":"arg2Of","subj":"EnjuParser_T187","obj":"EnjuParser_T182"},{"id":"EnjuParser_R183","pred":"arg1Of","subj":"EnjuParser_T183","obj":"EnjuParser_T184"},{"id":"EnjuParser_R184","pred":"arg2Of","subj":"EnjuParser_T185","obj":"EnjuParser_T184"},{"id":"EnjuParser_R185","pred":"arg3Of","subj":"EnjuParser_T186","obj":"EnjuParser_T184"},{"id":"EnjuParser_R186","pred":"arg1Of","subj":"EnjuParser_T183","obj":"EnjuParser_T187"},{"id":"EnjuParser_R187","pred":"arg2Of","subj":"EnjuParser_T189","obj":"EnjuParser_T187"},{"id":"EnjuParser_R188","pred":"arg1Of","subj":"EnjuParser_T189","obj":"EnjuParser_T188"},{"id":"EnjuParser_R189","pred":"arg1Of","subj":"EnjuParser_T187","obj":"EnjuParser_T190"},{"id":"EnjuParser_R190","pred":"arg1Of","subj":"EnjuParser_T190","obj":"EnjuParser_T191"},{"id":"EnjuParser_R191","pred":"arg2Of","subj":"EnjuParser_T194","obj":"EnjuParser_T191"},{"id":"EnjuParser_R192","pred":"arg1Of","subj":"EnjuParser_T194","obj":"EnjuParser_T192"},{"id":"EnjuParser_R193","pred":"arg1Of","subj":"EnjuParser_T194","obj":"EnjuParser_T193"},{"id":"EnjuParser_R194","pred":"arg1Of","subj":"EnjuParser_T194","obj":"EnjuParser_T195"},{"id":"EnjuParser_R195","pred":"arg2Of","subj":"EnjuParser_T197","obj":"EnjuParser_T195"},{"id":"EnjuParser_R196","pred":"arg3Of","subj":"EnjuParser_T198","obj":"EnjuParser_T195"},{"id":"EnjuParser_R197","pred":"arg1Of","subj":"EnjuParser_T197","obj":"EnjuParser_T196"},{"id":"EnjuParser_R198","pred":"arg1Of","subj":"EnjuParser_T200","obj":"EnjuParser_T199"},{"id":"EnjuParser_R199","pred":"arg1Of","subj":"EnjuParser_T200","obj":"EnjuParser_T201"},{"id":"EnjuParser_R200","pred":"arg2Of","subj":"EnjuParser_T205","obj":"EnjuParser_T201"},{"id":"EnjuParser_R201","pred":"arg1Of","subj":"EnjuParser_T205","obj":"EnjuParser_T202"},{"id":"EnjuParser_R202","pred":"arg1Of","subj":"EnjuParser_T202","obj":"EnjuParser_T203"},{"id":"EnjuParser_R203","pred":"arg2Of","subj":"EnjuParser_T204","obj":"EnjuParser_T203"},{"id":"EnjuParser_R204","pred":"arg1Of","subj":"EnjuParser_T205","obj":"EnjuParser_T204"},{"id":"EnjuParser_R205","pred":"arg1Of","subj":"EnjuParser_T200","obj":"EnjuParser_T206"},{"id":"EnjuParser_R206","pred":"arg2Of","subj":"EnjuParser_T215","obj":"EnjuParser_T206"},{"id":"EnjuParser_R207","pred":"arg1Of","subj":"EnjuParser_T215","obj":"EnjuParser_T207"},{"id":"EnjuParser_R208","pred":"arg1Of","subj":"EnjuParser_T210","obj":"EnjuParser_T208"},{"id":"EnjuParser_R209","pred":"arg1Of","subj":"EnjuParser_T210","obj":"EnjuParser_T209"},{"id":"EnjuParser_R210","pred":"arg1Of","subj":"EnjuParser_T210","obj":"EnjuParser_T211"},{"id":"EnjuParser_R211","pred":"arg2Of","subj":"EnjuParser_T214","obj":"EnjuParser_T211"},{"id":"EnjuParser_R212","pred":"arg1Of","subj":"EnjuParser_T214","obj":"EnjuParser_T212"},{"id":"EnjuParser_R213","pred":"arg1Of","subj":"EnjuParser_T214","obj":"EnjuParser_T213"},{"id":"EnjuParser_R214","pred":"arg1Of","subj":"EnjuParser_T211","obj":"EnjuParser_T215"},{"id":"EnjuParser_R215","pred":"arg2Of","subj":"EnjuParser_T216","obj":"EnjuParser_T215"},{"id":"EnjuParser_R216","pred":"arg1Of","subj":"EnjuParser_T211","obj":"EnjuParser_T216"},{"id":"EnjuParser_R217","pred":"arg1Of","subj":"EnjuParser_T216","obj":"EnjuParser_T217"},{"id":"EnjuParser_R218","pred":"arg2Of","subj":"EnjuParser_T219","obj":"EnjuParser_T217"},{"id":"EnjuParser_R219","pred":"arg1Of","subj":"EnjuParser_T219","obj":"EnjuParser_T218"},{"id":"EnjuParser_R220","pred":"arg1Of","subj":"EnjuParser_T219","obj":"EnjuParser_T220"},{"id":"EnjuParser_R221","pred":"arg2Of","subj":"EnjuParser_T222","obj":"EnjuParser_T220"},{"id":"EnjuParser_R222","pred":"arg1Of","subj":"EnjuParser_T222","obj":"EnjuParser_T221"},{"id":"EnjuParser_R223","pred":"arg1Of","subj":"EnjuParser_T224","obj":"EnjuParser_T223"},{"id":"EnjuParser_R224","pred":"arg1Of","subj":"EnjuParser_T224","obj":"EnjuParser_T225"},{"id":"EnjuParser_R225","pred":"arg2Of","subj":"EnjuParser_T226","obj":"EnjuParser_T225"},{"id":"EnjuParser_R226","pred":"arg2Of","subj":"EnjuParser_T224","obj":"EnjuParser_T226"},{"id":"EnjuParser_R227","pred":"arg1Of","subj":"EnjuParser_T226","obj":"EnjuParser_T227"},{"id":"EnjuParser_R228","pred":"arg2Of","subj":"EnjuParser_T230","obj":"EnjuParser_T227"},{"id":"EnjuParser_R229","pred":"arg1Of","subj":"EnjuParser_T230","obj":"EnjuParser_T228"},{"id":"EnjuParser_R230","pred":"arg1Of","subj":"EnjuParser_T230","obj":"EnjuParser_T229"},{"id":"EnjuParser_R231","pred":"arg2Of","subj":"EnjuParser_T230","obj":"EnjuParser_T231"},{"id":"EnjuParser_R232","pred":"arg1Of","subj":"EnjuParser_T231","obj":"EnjuParser_T232"},{"id":"EnjuParser_R233","pred":"arg2Of","subj":"EnjuParser_T233","obj":"EnjuParser_T232"},{"id":"EnjuParser_R234","pred":"arg1Of","subj":"EnjuParser_T235","obj":"EnjuParser_T234"},{"id":"EnjuParser_R235","pred":"arg1Of","subj":"EnjuParser_T235","obj":"EnjuParser_T236"},{"id":"EnjuParser_R236","pred":"arg2Of","subj":"EnjuParser_T243","obj":"EnjuParser_T236"},{"id":"EnjuParser_R237","pred":"arg1Of","subj":"EnjuParser_T243","obj":"EnjuParser_T237"},{"id":"EnjuParser_R238","pred":"arg1Of","subj":"EnjuParser_T238","obj":"EnjuParser_T239"},{"id":"EnjuParser_R239","pred":"arg2Of","subj":"EnjuParser_T241","obj":"EnjuParser_T239"},{"id":"EnjuParser_R240","pred":"arg1Of","subj":"EnjuParser_T241","obj":"EnjuParser_T240"},{"id":"EnjuParser_R241","pred":"arg1Of","subj":"EnjuParser_T239","obj":"EnjuParser_T242"},{"id":"EnjuParser_R242","pred":"arg2Of","subj":"EnjuParser_T243","obj":"EnjuParser_T242"},{"id":"EnjuParser_R243","pred":"arg1Of","subj":"EnjuParser_T239","obj":"EnjuParser_T243"},{"id":"EnjuParser_R244","pred":"arg2Of","subj":"EnjuParser_T246","obj":"EnjuParser_T243"},{"id":"EnjuParser_R245","pred":"arg1Of","subj":"EnjuParser_T246","obj":"EnjuParser_T244"},{"id":"EnjuParser_R246","pred":"arg1Of","subj":"EnjuParser_T246","obj":"EnjuParser_T245"},{"id":"EnjuParser_R247","pred":"arg1Of","subj":"EnjuParser_T243","obj":"EnjuParser_T247"},{"id":"EnjuParser_R248","pred":"arg2Of","subj":"EnjuParser_T250","obj":"EnjuParser_T247"},{"id":"EnjuParser_R249","pred":"arg1Of","subj":"EnjuParser_T250","obj":"EnjuParser_T248"},{"id":"EnjuParser_R250","pred":"arg1Of","subj":"EnjuParser_T250","obj":"EnjuParser_T249"},{"id":"EnjuParser_R251","pred":"arg1Of","subj":"EnjuParser_T250","obj":"EnjuParser_T251"},{"id":"EnjuParser_R252","pred":"arg2Of","subj":"EnjuParser_T255","obj":"EnjuParser_T251"},{"id":"EnjuParser_R253","pred":"arg1Of","subj":"EnjuParser_T255","obj":"EnjuParser_T252"},{"id":"EnjuParser_R254","pred":"arg1Of","subj":"EnjuParser_T255","obj":"EnjuParser_T253"},{"id":"EnjuParser_R255","pred":"arg1Of","subj":"EnjuParser_T255","obj":"EnjuParser_T254"},{"id":"EnjuParser_R256","pred":"arg1Of","subj":"EnjuParser_T250","obj":"EnjuParser_T256"},{"id":"EnjuParser_R257","pred":"arg2Of","subj":"EnjuParser_T259","obj":"EnjuParser_T256"},{"id":"EnjuParser_R258","pred":"arg1Of","subj":"EnjuParser_T259","obj":"EnjuParser_T257"},{"id":"EnjuParser_R259","pred":"arg1Of","subj":"EnjuParser_T259","obj":"EnjuParser_T258"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos600-GlycoProteins","denotations":[{"id":"PD-GlycoProteins-B_T1","span":{"begin":128,"end":130},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T2","span":{"begin":271,"end":273},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T3","span":{"begin":565,"end":567},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T4","span":{"begin":700,"end":702},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T5","span":{"begin":1342,"end":1344},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T6","span":{"begin":1394,"end":1396},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T7","span":{"begin":1583,"end":1585},"obj":"http://purl.uniprot.org/uniprot/P35914"},{"id":"PD-GlycoProteins-B_T8","span":{"begin":128,"end":130},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T9","span":{"begin":271,"end":273},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T10","span":{"begin":565,"end":567},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T11","span":{"begin":700,"end":702},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T12","span":{"begin":1342,"end":1344},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T13","span":{"begin":1394,"end":1396},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T14","span":{"begin":1583,"end":1585},"obj":"http://purl.uniprot.org/uniprot/O81027"},{"id":"PD-GlycoProteins-B_T15","span":{"begin":249,"end":253},"obj":"https://acgg.asia/db/gpdb/id/GPDB0005542"},{"id":"PD-GlycoProteins-B_T16","span":{"begin":249,"end":253},"obj":"https://acgg.asia/db/gpdb/id/GPDB0005543"},{"id":"PD-GlycoProteins-B_T17","span":{"begin":249,"end":253},"obj":"https://acgg.asia/db/gpdb/id/GPDB0005544"},{"id":"PD-GlycoProteins-B_T18","span":{"begin":249,"end":253},"obj":"https://acgg.asia/db/gpdb/id/GPDB0005545"},{"id":"PD-GlycoProteins-B_T19","span":{"begin":193,"end":236},"obj":"http://purl.uniprot.org/uniprot/P13721"},{"id":"PD-GlycoProteins-B_T20","span":{"begin":193,"end":236},"obj":"http://purl.uniprot.org/uniprot/P15907"},{"id":"PD-GlycoProteins-B_T21","span":{"begin":193,"end":236},"obj":"http://purl.uniprot.org/uniprot/Q76K27"},{"id":"PD-GlycoProteins-B_T22","span":{"begin":193,"end":236},"obj":"http://purl.uniprot.org/uniprot/Q96JF0"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyCosmos600-GlycoEpitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":383,"end":385},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":810,"end":812},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"},{"id":"PD-GlycoEpitope-B_T3","span":{"begin":1029,"end":1031},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":446,"end":448},"obj":"https://acgg.asia/db/lfdb/LfDB0344"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":383,"end":385},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":810,"end":812},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"},{"id":"PD-GlycoEpitope-B_T3","span":{"begin":1029,"end":1031},"obj":"http://www.glycoepitope.jp/epitopes/AN0281"}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}

{"project":"GlyTouCan-IUPAC","denotations":[{"id":"GlycanIUPAC_T1","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G52626JU\""},{"id":"GlycanIUPAC_T2","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G52626JU\""},{"id":"GlycanIUPAC_T3","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G52626JU\""},{"id":"GlycanIUPAC_T4","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G44803MF\""},{"id":"GlycanIUPAC_T5","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G44803MF\""},{"id":"GlycanIUPAC_T6","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G44803MF\""},{"id":"GlycanIUPAC_T7","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G75769XJ\""},{"id":"GlycanIUPAC_T8","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G75769XJ\""},{"id":"GlycanIUPAC_T9","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G75769XJ\""},{"id":"GlycanIUPAC_T10","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G32075UV\""},{"id":"GlycanIUPAC_T11","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G32075UV\""},{"id":"GlycanIUPAC_T12","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G32075UV\""},{"id":"GlycanIUPAC_T13","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G19871KB\""},{"id":"GlycanIUPAC_T14","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G19871KB\""},{"id":"GlycanIUPAC_T15","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G19871KB\""},{"id":"GlycanIUPAC_T16","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G02297CB\""},{"id":"GlycanIUPAC_T17","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G02297CB\""},{"id":"GlycanIUPAC_T18","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G02297CB\""},{"id":"GlycanIUPAC_T19","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G86711IL\""},{"id":"GlycanIUPAC_T20","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G86711IL\""},{"id":"GlycanIUPAC_T21","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G86711IL\""},{"id":"GlycanIUPAC_T22","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G29641QF\""},{"id":"GlycanIUPAC_T23","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G29641QF\""},{"id":"GlycanIUPAC_T24","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G29641QF\""},{"id":"GlycanIUPAC_T25","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G81991PU\""},{"id":"GlycanIUPAC_T26","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G81991PU\""},{"id":"GlycanIUPAC_T27","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G81991PU\""},{"id":"GlycanIUPAC_T28","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G50843NR\""},{"id":"GlycanIUPAC_T29","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G50843NR\""},{"id":"GlycanIUPAC_T30","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G50843NR\""},{"id":"GlycanIUPAC_T31","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G72982UM\""},{"id":"GlycanIUPAC_T32","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G72982UM\""},{"id":"GlycanIUPAC_T33","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G72982UM\""},{"id":"GlycanIUPAC_T34","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G99079GA\""},{"id":"GlycanIUPAC_T35","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G99079GA\""},{"id":"GlycanIUPAC_T36","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G99079GA\""},{"id":"GlycanIUPAC_T37","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G45378TF\""},{"id":"GlycanIUPAC_T38","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G45378TF\""},{"id":"GlycanIUPAC_T39","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G45378TF\""},{"id":"GlycanIUPAC_T40","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G57506TF\""},{"id":"GlycanIUPAC_T41","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G57506TF\""},{"id":"GlycanIUPAC_T42","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G57506TF\""},{"id":"GlycanIUPAC_T43","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G07340KF\""},{"id":"GlycanIUPAC_T44","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G07340KF\""},{"id":"GlycanIUPAC_T45","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G07340KF\""},{"id":"GlycanIUPAC_T46","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G64568HT\""},{"id":"GlycanIUPAC_T47","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G64568HT\""},{"id":"GlycanIUPAC_T48","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G64568HT\""},{"id":"GlycanIUPAC_T49","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G46846LC\""},{"id":"GlycanIUPAC_T50","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G46846LC\""},{"id":"GlycanIUPAC_T51","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G46846LC\""},{"id":"GlycanIUPAC_T52","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G77011RU\""},{"id":"GlycanIUPAC_T53","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G77011RU\""},{"id":"GlycanIUPAC_T54","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G77011RU\""},{"id":"GlycanIUPAC_T55","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G31033PX\""},{"id":"GlycanIUPAC_T56","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G31033PX\""},{"id":"GlycanIUPAC_T57","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G31033PX\""},{"id":"GlycanIUPAC_T58","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G16221YF\""},{"id":"GlycanIUPAC_T59","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G16221YF\""},{"id":"GlycanIUPAC_T60","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G16221YF\""},{"id":"GlycanIUPAC_T61","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G61143JT\""},{"id":"GlycanIUPAC_T62","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G61143JT\""},{"id":"GlycanIUPAC_T63","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G61143JT\""},{"id":"GlycanIUPAC_T64","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G51234LG\""},{"id":"GlycanIUPAC_T65","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G51234LG\""},{"id":"GlycanIUPAC_T66","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G51234LG\""},{"id":"GlycanIUPAC_T67","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G61307AH\""},{"id":"GlycanIUPAC_T68","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G61307AH\""},{"id":"GlycanIUPAC_T69","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G61307AH\""},{"id":"GlycanIUPAC_T70","span":{"begin":1155,"end":1158},"obj":"\"http://rdf.glycoinfo.org/glycan/G57624ZE\""},{"id":"GlycanIUPAC_T71","span":{"begin":1237,"end":1240},"obj":"\"http://rdf.glycoinfo.org/glycan/G57624ZE\""},{"id":"GlycanIUPAC_T72","span":{"begin":1284,"end":1287},"obj":"\"http://rdf.glycoinfo.org/glycan/G57624ZE\""}],"text":"Transcriptional regulation of human beta-galactoside alpha2, 6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line.\nWe have previously shown that the expression of beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5'-upstream of exon Y (A. Taniguchi et al., FEBS Lett.,441, 191-194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le(x)in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation."}