PubMed:10800163
Annnotations
PubMed_Structured_Abstracts
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 130-718 | OBJECTIVE | denotes | It has been established that cytogenetic findings at the time of diagnosis of acute myeloid leukemia (AML) are powerful prognostic indicators. Pericentric inversion of chromosome 16 and translocation t(16;16) resulting in chimeric fusion of CBFB and MYH11 genes are typically seen in the M4-Eo FAB classification subset of AML and are associated with low-risk disease. These subtle chromosomal abnormalities may be difficult to detect in poor-quality metaphase preparations and if missed could lead to incorrect assignment to risk groups and influence the therapy decision-making process. |
| T2 | 739-1121 | METHODS | denotes | We prospectively studied, at diagnosis, 10 patients with AML-M4 Eo by cytogenetics and fluorescent in situ hybridization (FISH) with two cosmids (36 and 40). As a control group, 7 patients (5 with a diagnosis of AML other than M4 Eo and two cases of reactive eosinophilia) were analyzed. In addition reverse transcriptase chain reaction (RT-PCR) studies were carried out in 6 cases. |
| T3 | 1131-1573 | RESULTS | denotes | Karyotypic analysis detected the inv(16) in all but one of the patients with M4-Eo while none of the control cases showed any abnormality on chromosome 16. FISH studies showed that all 10 patients had abnormalities on chromosome 16; the patient with normal karyotype showed an inv(16) by FISH, while a case with inv(16) by cytogenetics had a t(16;16) by FISH. RT-PCR demonstrated amplification of the CBFB/MYH11 product in all cases analyzed. |
| T4 | 1606-1790 | CONCLUSIONS | denotes | In patients with M4Eo and rearrangements of chromosome 16, FISH studies may afford more complete information than conventional cytogenetics and can be an alternative to RT-PCR studies. |
PubmedHPO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 208-230 | HP_0004808 | denotes | acute myeloid leukemia |
| T2 | 214-230 | HP_0012324 | denotes | myeloid leukemia |
| T3 | 222-230 | HP_0001909 | denotes | leukemia |
DisGeNET5_gene_disease
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 10800163-2#98#102#gene865 | 371-375 | gene865 | denotes | CBFB |
| 10800163-2#107#112#gene4629 | 380-385 | gene4629 | denotes | MYH11 |
| 10800163-2#151#154#gene2187 | 424-427 | gene2187 | denotes | FAB |
| 10800163-2#180#183#diseaseC0023467 | 453-456 | diseaseC0023467 | denotes | AML |
| 98#102#gene865180#183#diseaseC0023467 | 10800163-2#98#102#gene865 | 10800163-2#180#183#diseaseC0023467 | associated_with | CBFB,AML |
| 107#112#gene4629180#183#diseaseC0023467 | 10800163-2#107#112#gene4629 | 10800163-2#180#183#diseaseC0023467 | associated_with | MYH11,AML |
| 151#154#gene2187180#183#diseaseC0023467 | 10800163-2#151#154#gene2187 | 10800163-2#180#183#diseaseC0023467 | associated_with | FAB,AML |
DisGeNET
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T0 | 371-375 | gene:865 | denotes | CBFB |
| T1 | 453-456 | disease:C0023467 | denotes | AML |
| T2 | 380-385 | gene:4629 | denotes | MYH11 |
| T3 | 453-456 | disease:C0023467 | denotes | AML |
| T4 | 424-427 | gene:2187 | denotes | FAB |
| T5 | 453-456 | disease:C0023467 | denotes | AML |
| R1 | T0 | T1 | associated_with | CBFB,AML |
| R2 | T2 | T3 | associated_with | MYH11,AML |
| R3 | T4 | T5 | associated_with | FAB,AML |