PubMed:10799457 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":47,"end":66},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":171,"end":190},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":321,"end":332},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":437,"end":462},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":464,"end":467},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":508,"end":511},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":1165,"end":1168},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":1292,"end":1295},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1351,"end":1360},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1408,"end":1411},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1454,"end":1465},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":1599,"end":1615},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T13","span":{"begin":1646,"end":1662},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T14","span":{"begin":1664,"end":1666},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T16","span":{"begin":1685,"end":1687},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T18","span":{"begin":1860,"end":1869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T19","span":{"begin":1997,"end":2012},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0005055"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0005055"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0000605"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0018842"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0018842"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0012833"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0018842"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0018842"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0000605"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"0018842"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"0000605"},{"id":"A12","pred":"mondo_id","subj":"T12","obj":"0021094"},{"id":"A13","pred":"mondo_id","subj":"T13","obj":"0005055"},{"id":"A14","pred":"mondo_id","subj":"T14","obj":"0005055"},{"id":"A15","pred":"mondo_id","subj":"T14","obj":"0008260"},{"id":"A16","pred":"mondo_id","subj":"T16","obj":"0005055"},{"id":"A17","pred":"mondo_id","subj":"T16","obj":"0008260"},{"id":"A18","pred":"mondo_id","subj":"T18","obj":"0000605"},{"id":"A19","pred":"mondo_id","subj":"T19","obj":"0005187"}],"text":"New immunofluorescence assays for detection of Human herpesvirus 8-specific antibodies.\nSeveral assays have been developed for detection of immunoglobulin G antibodies to Human herpesvirus 8 (HHV-8), including immunofluorescence assays (IFAs) and enzyme-linked immunosorbent assays (ELISAs). However, the specificity and sensitivity of these assays are not completely defined due to the lack of a \"gold standard.\" Although IFAs based on primary effusion lymphoma (PEL) cell lines are used widely, the assays can be confounded by nonspecific reactions against cellular components and potential cross-reaction with antibodies against other herpesviruses. To provide more reliable IFAs, we established recombinant Semliki Forest viruses (rSFVs) expressing the HHV-8-specific proteins ORF73 and K8.1 and used BHK-21 cells infected with these rSFVs for IFA (ORF73-IFA and K8.1-IFA). Expression of the HHV-8-specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity. The rSFV system also allowed detection of antibodies against glycosylation-dependent epitopes of K8.1. Titers measured by rSFV-based IFAs and PEL-based IFAs correlated well (correlation coefficients of \u003e0.9), and concordances of seroreactivities between rSFV-based and PEL-based IFAs were \u003e97% (kappa \u003e 0.93). K8.1-IFA was more sensitive than either ORF73-IFA or peptide ELISAs. Using PEL-based lytic IFA as a reference assay, the sensitivity and specificity of K8.1-IFA were estimated to be 94 and 100%, respectively. HHV-8 prevalences determined by K8.1-IFA among the human immunodeficiency virus (HIV)-positive (HIV(+)) Kaposi's sarcoma (KS) patients, HIV(+) KS(-) patients, and healthy controls were 100, 65, and 6.7%, respectively, which were consistent with prior reports. Therefore, our rSFV-based IFAs may provide a specific and sensitive method for use in epidemiology studies. In addition, they will provide a basis for further development of diagnostic tests for HHV-8 infection."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":454,"end":462},"obj":"HP_0002665"},{"id":"T2","span":{"begin":1599,"end":1615},"obj":"HP_0002721"},{"id":"T3","span":{"begin":1646,"end":1662},"obj":"HP_0100726"},{"id":"T4","span":{"begin":1655,"end":1662},"obj":"HP_0100242"}],"text":"New immunofluorescence assays for detection of Human herpesvirus 8-specific antibodies.\nSeveral assays have been developed for detection of immunoglobulin G antibodies to Human herpesvirus 8 (HHV-8), including immunofluorescence assays (IFAs) and enzyme-linked immunosorbent assays (ELISAs). However, the specificity and sensitivity of these assays are not completely defined due to the lack of a \"gold standard.\" Although IFAs based on primary effusion lymphoma (PEL) cell lines are used widely, the assays can be confounded by nonspecific reactions against cellular components and potential cross-reaction with antibodies against other herpesviruses. To provide more reliable IFAs, we established recombinant Semliki Forest viruses (rSFVs) expressing the HHV-8-specific proteins ORF73 and K8.1 and used BHK-21 cells infected with these rSFVs for IFA (ORF73-IFA and K8.1-IFA). Expression of the HHV-8-specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity. The rSFV system also allowed detection of antibodies against glycosylation-dependent epitopes of K8.1. Titers measured by rSFV-based IFAs and PEL-based IFAs correlated well (correlation coefficients of \u003e0.9), and concordances of seroreactivities between rSFV-based and PEL-based IFAs were \u003e97% (kappa \u003e 0.93). K8.1-IFA was more sensitive than either ORF73-IFA or peptide ELISAs. Using PEL-based lytic IFA as a reference assay, the sensitivity and specificity of K8.1-IFA were estimated to be 94 and 100%, respectively. HHV-8 prevalences determined by K8.1-IFA among the human immunodeficiency virus (HIV)-positive (HIV(+)) Kaposi's sarcoma (KS) patients, HIV(+) KS(-) patients, and healthy controls were 100, 65, and 6.7%, respectively, which were consistent with prior reports. Therefore, our rSFV-based IFAs may provide a specific and sensitive method for use in epidemiology studies. In addition, they will provide a basis for further development of diagnostic tests for HHV-8 infection."}

{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":781,"end":786},"obj":"Protein"},{"id":"T2","span":{"begin":791,"end":795},"obj":"Protein"},{"id":"T3","span":{"begin":853,"end":858},"obj":"Protein"},{"id":"T4","span":{"begin":867,"end":871},"obj":"Protein"},{"id":"T5","span":{"begin":1120,"end":1124},"obj":"Protein"},{"id":"T6","span":{"begin":1373,"end":1378},"obj":"Protein"},{"id":"T7","span":{"begin":1485,"end":1489},"obj":"Protein"},{"id":"T8","span":{"begin":1574,"end":1578},"obj":"Protein"}],"text":"New immunofluorescence assays for detection of Human herpesvirus 8-specific antibodies.\nSeveral assays have been developed for detection of immunoglobulin G antibodies to Human herpesvirus 8 (HHV-8), including immunofluorescence assays (IFAs) and enzyme-linked immunosorbent assays (ELISAs). However, the specificity and sensitivity of these assays are not completely defined due to the lack of a \"gold standard.\" Although IFAs based on primary effusion lymphoma (PEL) cell lines are used widely, the assays can be confounded by nonspecific reactions against cellular components and potential cross-reaction with antibodies against other herpesviruses. To provide more reliable IFAs, we established recombinant Semliki Forest viruses (rSFVs) expressing the HHV-8-specific proteins ORF73 and K8.1 and used BHK-21 cells infected with these rSFVs for IFA (ORF73-IFA and K8.1-IFA). Expression of the HHV-8-specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity. The rSFV system also allowed detection of antibodies against glycosylation-dependent epitopes of K8.1. Titers measured by rSFV-based IFAs and PEL-based IFAs correlated well (correlation coefficients of \u003e0.9), and concordances of seroreactivities between rSFV-based and PEL-based IFAs were \u003e97% (kappa \u003e 0.93). K8.1-IFA was more sensitive than either ORF73-IFA or peptide ELISAs. Using PEL-based lytic IFA as a reference assay, the sensitivity and specificity of K8.1-IFA were estimated to be 94 and 100%, respectively. HHV-8 prevalences determined by K8.1-IFA among the human immunodeficiency virus (HIV)-positive (HIV(+)) Kaposi's sarcoma (KS) patients, HIV(+) KS(-) patients, and healthy controls were 100, 65, and 6.7%, respectively, which were consistent with prior reports. Therefore, our rSFV-based IFAs may provide a specific and sensitive method for use in epidemiology studies. In addition, they will provide a basis for further development of diagnostic tests for HHV-8 infection."}