| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-128 |
Sentence |
denotes |
Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins. |
| T2 |
129-235 |
Sentence |
denotes |
Many eukaryotic cell surface proteins are anchored to the membrane via glycosylphosphatidylinositol (GPI). |
| T3 |
236-335 |
Sentence |
denotes |
The GPI is attached to proteins that have a GPI attachment signal peptide at the carboxyl terminus. |
| T4 |
336-511 |
Sentence |
denotes |
The GPI attachment signal peptide is replaced by a preassembled GPI in the endoplasmic reticulum by a transamidation reaction through the formation of a carbonyl intermediate. |
| T5 |
512-584 |
Sentence |
denotes |
GPI transamidase is a key enzyme of this posttranslational modification. |
| T6 |
585-658 |
Sentence |
denotes |
Here we report that Gaa1p and Gpi8p are components of a GPI transamidase. |
| T7 |
659-765 |
Sentence |
denotes |
To determine a role of Gaa1p we disrupted a GAA1/GPAA1 gene in mouse F9 cells by homologous recombination. |
| T8 |
766-937 |
Sentence |
denotes |
GAA1 knockout cells were defective in the formation of carbonyl intermediates between precursor proteins and transamidase as determined by an in vitro GPI-anchoring assay. |
| T9 |
938-1116 |
Sentence |
denotes |
We also show that cysteine and histidine residues of Gpi8p, which are conserved in members of a cysteine protease family, are essential for generation of a carbonyl intermediate. |
| T10 |
1117-1221 |
Sentence |
denotes |
This result suggests that Gpi8p is a catalytic component that cleaves the GPI attachment signal peptide. |
| T11 |
1222-1279 |
Sentence |
denotes |
Moreover, Gaa1p and Gpi8p are associated with each other. |
| T12 |
1280-1424 |
Sentence |
denotes |
Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment. |
