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PubMed:10791963 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-61 Sentence denotes Cytoplasmic, nuclear, and golgi localization of RGS proteins.
T2 62-145 Sentence denotes Evidence for N-terminal and RGS domain sequences as intracellular targeting motifs.
T3 146-271 Sentence denotes RGS proteins comprise a family of proteins named for their ability to negatively regulate heterotrimeric G protein signaling.
T4 272-518 Sentence denotes Biochemical studies suggest that members of this protein family act as GTPase-activating proteins for certain Galpha subunits, thereby accelerating the turn-off mechanism of Galpha and terminating signaling by both Galpha and Gbetagamma subunits.
T5 519-763 Sentence denotes In the present study, we used confocal microscopy to examine the intracellular distribution of several RGS proteins in COS-7 cells expressing RGS-green fluorescent protein (GFP) fusion proteins and in cells expressing RGS proteins endogenously.
T6 764-871 Sentence denotes RGS2 and RGS10 accumulated in the nucleus of COS-7 cells transfected with GFP constructs of these proteins.
T7 872-952 Sentence denotes In contrast, RGS4 and RGS16 accumulated in the cytoplasm of COS-7 transfectants.
T8 953-1114 Sentence denotes As observed in COS-7 cells, RGS4 exhibited cytoplasmic localization in mouse neuroblastoma cells, and RGS10 exhibited nuclear localization in human glioma cells.
T9 1115-1361 Sentence denotes Deletion or alanine substitution of an N-terminal leucine repeat motif present in both RGS4 and RGS16, a domain identified as a nuclear export sequence in HIV Rev and other proteins, promoted nuclear localization of these proteins in COS-7 cells.
T10 1362-1565 Sentence denotes In agreement with this observation, treatment of mouse neuroblastoma cells with leptomycin B to inhibit nuclear protein export by exportin1 resulted in accumulation of RGS4 in the nucleus of these cells.
T11 1566-1744 Sentence denotes GFP fusions of RGS domains of RGS proteins localized in the nucleus, suggesting that nuclear localization of RGS proteins results from nuclear targeting via RGS domain sequences.
T12 1745-1873 Sentence denotes RGSZ, which shares with RGS-GAIP a cysteine-rich string in its N-terminal region, localized to the Golgi complex in COS-7 cells.
T13 1874-1987 Sentence denotes Deletion of the N-terminal domain of RGSZ that includes the cysteine motif promoted nuclear localization of RGSZ.
T14 1988-2060 Sentence denotes None of the RGS proteins examined were localized at the plasma membrane.
T15 2061-2229 Sentence denotes These results demonstrate that RGS proteins localize in the nucleus, the cytoplasm, or shuttle between the nucleus and cytoplasm as nucleo-cytoplasmic shuttle proteins.
T16 2230-2429 Sentence denotes RGS proteins localize differentially within cells as a result of structural differences among these proteins that do not appear to be important determinants for their G protein-regulating activities.
T17 2430-2542 Sentence denotes These findings suggest involvement of RGS proteins in more complex cellular functions than currently envisioned.

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 1030-1043 HP_0003006 denotes neuroblastoma
T2 1101-1107 HP_0009733 denotes glioma
T3 1417-1430 HP_0003006 denotes neuroblastoma

PennBioIE

Id Subject Object Predicate Lexical cue
T1 48-60 protein denotes RGS proteins
T2 90-93 protein denotes RGS
T3 147-158 protein denotes GS proteins
T4 236-260 protein denotes heterotrimeric G protein
T5 343-369 protein denotes GTPase-activating proteins
T6 382-388 protein denotes Galpha
T7 446-452 protein denotes Galpha
T8 487-493 protein denotes Galpha
T9 498-508 protein denotes Gbetagamma
T10 622-634 protein denotes RGS proteins
T11 661-664 protein denotes RGS
T12 665-690 protein denotes green fluorescent protein
T13 692-695 protein denotes GFP
T14 737-749 protein denotes RGS proteins
T15 764-768 protein denotes RGS2
T16 773-778 protein denotes RGS10
T17 838-841 protein denotes GFP
T18 885-889 protein denotes RGS4
T19 894-899 protein denotes RGS16
T20 981-985 protein denotes RGS4
T21 1030-1043 protein denotes neuroblastoma
T22 1055-1060 protein denotes RGS10
T23 1101-1107 protein denotes glioma
T24 1115-1123 protein denotes Deletion
T25 1127-1134 protein denotes alanine
T26 1135-1147 protein denotes substitution
T27 1165-1172 protein denotes leucine
T28 1202-1206 protein denotes RGS4
T29 1211-1216 protein denotes RGS16
T30 1274-1277 protein denotes Rev
T31 1417-1430 protein denotes neuroblastoma
T32 1492-1501 protein denotes exportin1
T33 1530-1534 protein denotes RGS4
T34 1566-1569 protein denotes GFP
T35 1581-1584 protein denotes RGS
T36 1596-1599 protein denotes RGS
T37 1675-1678 protein denotes RGS
T38 1723-1726 protein denotes RGS
T39 1745-1749 protein denotes RGSZ
T40 1769-1777 protein denotes RGS-GAIP
T41 1874-1882 protein denotes Deletion
T42 1911-1915 protein denotes RGSZ
T43 1982-1986 protein denotes RGSZ
T44 2000-2012 protein denotes RGS proteins
T45 2092-2104 protein denotes RGS proteins
T46 2230-2242 protein denotes RGS proteins
T47 2324-2338 protein denotes these proteins
T48 2397-2406 protein denotes G protein
T49 2468-2480 protein denotes RGS proteins

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 1030-1043 Disease denotes neuroblastoma http://purl.obolibrary.org/obo/MONDO_0005072
T2 1101-1107 Disease denotes glioma http://purl.obolibrary.org/obo/MONDO_0021042
T3 1417-1430 Disease denotes neuroblastoma http://purl.obolibrary.org/obo/MONDO_0005072

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1024-1029 OrganismTaxon denotes mouse 10088|10090
T3 1095-1100 OrganismTaxon denotes human 9606
T4 1411-1416 OrganismTaxon denotes mouse 10088|10090

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 26-31 Body_part denotes golgi http://purl.obolibrary.org/obo/GO_0005794
T2 114-127 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T3 584-597 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T4 798-805 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T6 919-928 Body_part denotes cytoplasm http://purl.obolibrary.org/obo/GO_0005737
T7 996-1007 Body_part denotes cytoplasmic http://purl.obolibrary.org/obo/GO_0005737
T8 1542-1549 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T10 1626-1633 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T12 1844-1857 Body_part denotes Golgi complex http://purl.obolibrary.org/obo/GO_0005794
T13 2044-2059 Body_part denotes plasma membrane http://purl.obolibrary.org/obo/GO_0005886
T14 2121-2128 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T16 2134-2143 Body_part denotes cytoplasm http://purl.obolibrary.org/obo/GO_0005737
T17 2168-2175 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T19 2180-2189 Body_part denotes cytoplasm http://purl.obolibrary.org/obo/GO_0005737
T20 2193-2211 Body_part denotes nucleo-cytoplasmic http://purl.obolibrary.org/obo/GO_0005622

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 1030-1043 Phenotype denotes neuroblastoma HP:0003006
T2 1101-1107 Phenotype denotes glioma HP:0009733
T3 1417-1430 Phenotype denotes neuroblastoma HP:0003006