| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-50 |
Sentence |
denotes |
TatD is a cytoplasmic protein with DNase activity. |
| T2 |
51-125 |
Sentence |
denotes |
No requirement for TatD family proteins in sec-independent protein export. |
| T3 |
126-250 |
Sentence |
denotes |
The Escherichia coli Tat system mediates Sec-independent export of protein precursors bearing twin arginine signal peptides. |
| T4 |
251-368 |
Sentence |
denotes |
Genes known to be involved in this process include tatA, tatB, and tatC that form an operon with a fourth gene, tatD. |
| T5 |
369-463 |
Sentence |
denotes |
The tatD gene product has two homologues in E. coli coded by the unlinked ycfH and yjjV genes. |
| T6 |
464-703 |
Sentence |
denotes |
An E. coli strain with in-frame chromosomal deletions in all three of tatD, ycfH, and yjjV exhibits no significant defect in the cellular location of five cofactor-containing enzymes that are synthesized with twin arginine signal peptides. |
| T7 |
704-866 |
Sentence |
denotes |
Neither these mutations nor overproduction of the TatD protein cause any discernible effect on the export kinetics of an additional E. coli Tat pathway substrate. |
| T8 |
867-981 |
Sentence |
denotes |
It is concluded that proteins of the TatD family have no obligate involvement in protein export by the Tat system. |
| T9 |
982-1024 |
Sentence |
denotes |
TatD is shown to be a cytoplasmic protein. |
| T10 |
1025-1133 |
Sentence |
denotes |
TatD binds to immobilized Ni(2+) or Zn(2+) affinity columns and exhibits magnesium-dependent DNase activity. |
| T11 |
1134-1209 |
Sentence |
denotes |
Features of the tatA operon that may control TatD expression are discussed. |
