PubMed:10744032 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10744032","sourcedb":"PubMed","sourceid":"10744032","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10744032","text":"Transforming growth factor-beta1 stimulates contraction of human glioblastoma cell-mediated collagen lattice through enhanced alpha2 integrin expression.\nRapid invasiveness is a feature of the highly malignant glioblastoma tumor and is closely related to patient prognosis. The interaction between extracellular matrix (ECM) and cell surface receptors such as integrin heterodimers play a key role in the process of tumor invasion. We investigated the effects of transforming growth factor-beta1 (TGF-beta1), which is a mitogenic factor for glial cells, on integrin expression in T98G human glioblastoma cells using an in vitro model 3-dimensional collagen lattice. Exogenously applied TGF-beta1 dose-dependently enhanced collagen lattice contraction. Among the inhibitory antibodies tested against alpha integrin subunits, the anti-alpha2 antibody, P1-E6, alone prevented the enhanced contractile response by TGF-beta1, whereas any alpha integrin antibody (including P1-E6) had little effect on lattice contraction when cultured without TGF-beta1. RT-PCR analysis revealed that TGF-beta1 strongly increased alpha2 integrin transcript level. Furthermore, pretreatment with antisense phosphorothioate oligodeoxynucleotides against human alpha2 integrin using hemagglutinating virus of Japan (HVJ) liposome-mediated transfer prevented the effects of TGF-beta1 and also reduced the lattice contraction even in the absence of TGF-beta1. This data indicates that increased expression of alpha2 integrin is responsive to enhanced collagen lattice contraction by TGF-beta1. We suggest that TGF-beta1 exerts its effects on the invasive property of glioblastoma cells via upregulation of the alpha2 integrin subunit expression.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":210,"end":222},"obj":"HP_0100843"},{"id":"T2","span":{"begin":223,"end":228},"obj":"HP_0002664"},{"id":"T3","span":{"begin":416,"end":421},"obj":"HP_0002664"},{"id":"T4","span":{"begin":591,"end":603},"obj":"HP_0100843"},{"id":"T5","span":{"begin":739,"end":750},"obj":"HP_0001371"},{"id":"T6","span":{"begin":886,"end":897},"obj":"HP_0001371"},{"id":"T7","span":{"begin":1004,"end":1015},"obj":"HP_0001371"},{"id":"T8","span":{"begin":1387,"end":1398},"obj":"HP_0001371"},{"id":"T9","span":{"begin":1541,"end":1552},"obj":"HP_0001371"},{"id":"T10","span":{"begin":1640,"end":1652},"obj":"HP_0100843"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"},{"subj":"T4","pred":"source","obj":"PubmedHPO"},{"subj":"T5","pred":"source","obj":"PubmedHPO"},{"subj":"T6","pred":"source","obj":"PubmedHPO"},{"subj":"T7","pred":"source","obj":"PubmedHPO"},{"subj":"T8","pred":"source","obj":"PubmedHPO"},{"subj":"T9","pred":"source","obj":"PubmedHPO"},{"subj":"T10","pred":"source","obj":"PubmedHPO"}]},{"project":"Ab3P-abbreviations","denotations":[{"id":"SF0","span":{"begin":320,"end":323},"obj":"ABBR"},{"id":"LF0","span":{"begin":298,"end":318},"obj":"ABBR"},{"id":"SF1","span":{"begin":497,"end":506},"obj":"ABBR"},{"id":"LF1","span":{"begin":463,"end":495},"obj":"ABBR"},{"id":"SF2","span":{"begin":1291,"end":1294},"obj":"ABBR"},{"id":"LF2","span":{"begin":1258,"end":1289},"obj":"ABBR"}],"relations":[{"id":"R0","pred":"ShortForm","subj":"LF0","obj":"SF0"},{"id":"R1","pred":"ShortForm","subj":"LF1","obj":"SF1"},{"id":"R2","pred":"ShortForm","subj":"LF2","obj":"SF2"}],"attributes":[{"subj":"SF0","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"LF0","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"SF1","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"LF1","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"SF2","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"LF2","pred":"source","obj":"Ab3P-abbreviations"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#c5ec93","default":true},{"id":"Ab3P-abbreviations","color":"#ec93df"}]}]}}