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PubMed:10692419 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-108 Sentence denotes Identification and characterization of a novel cAMP receptor protein in the cyanobacterium Synechocystis sp.
T2 109-118 Sentence denotes PCC 6803.
T3 119-165 Sentence denotes Three open reading frames of Synechocystis sp.
T4 166-282 Sentence denotes PCC 6803 encoding a domain homologous with the cAMP binding domain of bacterial cAMP receptor protein were analyzed.
T5 283-563 Sentence denotes These three open reading frames, sll1371, sll1924, and slr0593, which were named sycrp1, sycrp2, and sypk, respectively, were expressed in Escherichia coli as His-tagged or glutathione S-transferase fusion proteins and purified, and their biochemical properties were investigated.
T6 564-740 Sentence denotes The results obtained for equilibrium dialysis measurements using these recombinant proteins suggest that SYCRP1 and SYPK show a binding affinity for cAMP while SYCRP2 does not.
T7 741-823 Sentence denotes The dissociation constant of His-tagged SYCRP1 for cAMP is approximately 3 microM.
T8 824-1037 Sentence denotes A cross-linking experiment using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide revealed that His-tagged SYCRP1 forms a homodimer, and the presence or absence of cAMP does not affect the formation of the homodimer.
T9 1038-1192 Sentence denotes The amino acid sequence reveals that SYCRP1 has a domain similar to the DNA binding domain of bacterial cAMP receptor protein in the COOH-terminal region.
T10 1193-1353 Sentence denotes Consistent with this, His-tagged SYCRP1 forms a complex with DNA that contains the consensus sequence for E. coli cAMP receptor protein in the presence of cAMP.
T11 1354-1430 Sentence denotes These results strongly suggest that SYCRP1 is a novel cAMP receptor protein.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 76-90 http://purl.obolibrary.org/obo/MAT_0000192 denotes cyanobacterium

CyanoBase

Id Subject Object Predicate Lexical cue
T1 316-323 protein denotes sll1371
T2 325-332 protein denotes sll1924
T3 338-345 protein denotes slr0593
T4 364-370 protein denotes sycrp1
T5 372-378 protein denotes sycrp2
T6 384-388 protein denotes sypk
T7 669-675 protein denotes SYCRP1
T8 680-684 protein denotes SYPK
T9 724-730 protein denotes SYCRP2
T10 781-787 protein denotes SYCRP1
T11 928-934 protein denotes SYCRP1
T12 1075-1081 protein denotes SYCRP1
T13 1226-1232 protein denotes SYCRP1
T14 1390-1396 protein denotes SYCRP1

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 109-112 Disease denotes PCC http://purl.obolibrary.org/obo/MONDO_0004974|http://purl.obolibrary.org/obo/MONDO_0022673
T3 166-169 Disease denotes PCC http://purl.obolibrary.org/obo/MONDO_0004974|http://purl.obolibrary.org/obo/MONDO_0022673

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 76-90 OrganismTaxon denotes cyanobacterium 102234
T2 91-104 OrganismTaxon denotes Synechocystis 1142
T3 148-161 OrganismTaxon denotes Synechocystis 1142
T4 422-438 OrganismTaxon denotes Escherichia coli 562
T5 601-609 OrganismTaxon denotes dialysis 124307
T6 1299-1306 OrganismTaxon denotes E. coli 562

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 76-90 Body_part denotes cyanobacterium http://purl.obolibrary.org/obo/MAT_0000192