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PubMed:10692350 JSONTXT

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Id Subject Object Predicate Lexical cue
T1 0-133 Sentence denotes Three new NifA-regulated genes in the Bradyrhizobium japonicum symbiotic gene region discovered by competitive DNA-RNA hybridization.
T2 134-211 Sentence denotes The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C.
T3 212-222 Sentence denotes Kündig, H.
T4 223-239 Sentence denotes Hennecke, and M.
T5 240-252 Sentence denotes Göttfert, J.
T6 253-263 Sentence denotes Bacteriol.
T7 264-382 Sentence denotes 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA.
T8 383-736 Sentence denotes Southern blots of restriction enzyme-digested cosmids that represent an ordered, overlapping library of the symbiotic region were competitively hybridized with in vitro-labeled RNA from anaerobically grown wild-type cells and an excess of RNA isolated either from anaerobically grown nifA and rpoN mutant cells or from aerobically grown wild-type cells.
T9 737-927 Sentence denotes In addition to the previously characterized nif and fix gene clusters, we identified three new NifA-regulated genes that were named nrgA, nrgB, and nrgC (nrg stands for NifA-regulated gene).
T10 928-974 Sentence denotes The latter two probably form an operon, nrgBC.
T11 975-1119 Sentence denotes The proteins encoded by nrgC and nrgA exhibited amino acid sequence similarity to bacterial hydroxylases and N-acetyltransferases, respectively.
T12 1120-1210 Sentence denotes The product of nrgB showed no significant similarity to any protein with a database entry.
T13 1211-1472 Sentence denotes Primer extension experiments and expression studies with translational lacZ fusions revealed the presence of a functional -24/-12-type promoter upstream of nrgA and nrgBC and proved the NifA- and RpoN (sigma(54))-dependent transcription of the respective genes.
T14 1473-1684 Sentence denotes Null mutations introduced into nrgA and nrgBC resulted in mutant strains that exhibited wild-type-like symbiotic properties, including nitrogen fixation, when tested on soybean, cowpea, or mung bean host plants.
T15 1685-1876 Sentence denotes Thus, the discovery of nrgA and nrgBC further emphasizes the previously suggested role of NifA as an activator of anaerobically induced genes other than the classical nitrogen fixation genes.