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sentences

Id	Subject	Object	Predicate	Lexical cue
T1	0-105	Sentence	denotes	Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase.
T2	106-218	Sentence	denotes	UDP-GalNAc pyrophosphorylase (UDP-GalNAcPP; AGX1) catalyzes the synthesis of UDP-GalNAc from UTP and GalNAc-1-P.
T3	219-327	Sentence	denotes	The 475-amino acid protein (57 kDa protein) also synthesizes UDP-GlcNAc at about 25% the rate of UDP-GalNAc.
T4	328-511	Sentence	denotes	The cDNA for this enzyme, termed AGX1, was cloned in Escherichia coli, and expressed as an active enzyme that cross-reacted with antiserum against the original pig liver UDP-HexNAcPP.
T5	512-663	Sentence	denotes	In the present study, we incubated recombinant AGX1 with N(3)-UDP-[(32)P]GlcNAc and N(3)-UDP-[(32)P]GalNAc probes to label the nucleotide-binding site.
T6	664-869	Sentence	denotes	Proteolytic digestions of the labeled enzyme and analysis of the resulting peptides indicated that both photoprobes cross-linked to one 24-amino acid peptide located between residues Val(216) and Glu(240).
T7	870-1023	Sentence	denotes	Four amino acids in this peptide were found to be highly conserved among closely related enzymes, and each of these was individually modified to alanine.
T8	1024-1261	Sentence	denotes	Mutation of Gly(222) to Ala in the peptide almost completely eliminated UDP-GlcNAc and UDP-GalNAc synthesis, while mutation of Gly(224) to Ala, almost completely eliminated UDP-GalNAc synthesis, but UDP-GlcNAc was only diminished by 50%.
T9	1262-1426	Sentence	denotes	Both of these mutations also resulted in almost complete loss of the ability of the mutated proteins to cross-link N(3)-UDP-[(32)P]GlcNAc or N(3)-UDP-[(32)P]GalNAc.
T10	1427-1643	Sentence	denotes	On the other hand, mutations of either Pro(220) or Tyr(227) to Ala did not greatly affect enzymatic activity, although there was some reduction in the ability of these proteins to cross-link the photoaffinity probes.
T11	1644-1804	Sentence	denotes	We also mutated Gly(111) to Ala since this amino acid was reported to be necessary for catalysis (Mio, T., Yabe, T., Arisawa, M., and Yamada-Okabe, H. (1998) J.
T12	1805-1810	Sentence	denotes	Biol.
T13	1811-1816	Sentence	denotes	Chem.
T14	1817-1835	Sentence	denotes	273, 14392-14397).
T15	1836-2034	Sentence	denotes	The Gly(111) to Ala mutant lost all enzymatic activity, but interestingly enough, this mutant protein still cross-linked the radioactive N(3)-UDP-GlcNAc although not nearly as well as the wild type.
T16	2035-2197	Sentence	denotes	On the other hand, mutation of Arg(115) to Ala had no affect on enzymatic activity although it also reduced the amount of cross-linking of N(3)-UDP-[(32)P]GlcNAc.
T17	2198-2366	Sentence	denotes	These studies help to define essential amino acids at or near the nucleotide-binding site and the catalytic site, as well as peptides involved in binding and catalysis.

Glycosmos6-MAT

Id	Subject	Object	Predicate	Lexical cue
T1	492-497	http://purl.obolibrary.org/obo/MAT_0000097	denotes	liver
T2	1440-1444	http://purl.obolibrary.org/obo/MAT_0000091	denotes	hand
T3	2048-2052	http://purl.obolibrary.org/obo/MAT_0000091	denotes	hand

NGLY1-deficiency

Id	Subject	Object	Predicate	Lexical cue
PD-NGLY1-deficiency-B_T1	79-85	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T2	284-290	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T3	585-591	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T4	1100-1106	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T5	1227-1233	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T6	1393-1399	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T7	1982-1988	chem:24139	denotes	GlcNAc
PD-NGLY1-deficiency-B_T8	2190-2196	chem:24139	denotes	GlcNAc

Anatomy-MAT

Id	Subject	Object	Predicate	Lexical cue	mat_id
T1	492-497	Body_part	denotes	liver	http://purl.obolibrary.org/obo/MAT_0000097
T2	1440-1444	Body_part	denotes	hand	http://purl.obolibrary.org/obo/MAT_0000091
T3	2048-2052	Body_part	denotes	hand	http://purl.obolibrary.org/obo/MAT_0000091

Glycan-GlyCosmos

Id	Subject	Object	Predicate	Lexical cue	image
T1	71-77	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2	79-85	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T3	110-116	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T4	187-193	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T5	207-213	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T6	284-290	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T7	320-326	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T8	585-591	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T9	612-618	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T10	1100-1106	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T11	1115-1121	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T12	1201-1207	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T13	1227-1233	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T14	1393-1399	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T15	1419-1425	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T16	1982-1988	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T17	2190-2196	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

GlyCosmos15-NCBITAXON

Id	Subject	Object	Predicate	Lexical cue	db_id
T1	381-397	OrganismTaxon	denotes	Escherichia coli	562

GlyCosmos15-UBERON

Id	Subject	Object	Predicate	Lexical cue	uberon_id
T1	492-497	Body_part	denotes	liver	http://purl.obolibrary.org/obo/UBERON_0002107
T2	1440-1444	Body_part	denotes	hand	http://purl.obolibrary.org/obo/UBERON_0002398
T3	2048-2052	Body_part	denotes	hand	http://purl.obolibrary.org/obo/UBERON_0002398

GlyCosmos15-MAT

Id	Subject	Object	Predicate	Lexical cue	mat_id
T1	492-497	Body_part	denotes	liver	http://purl.obolibrary.org/obo/MAT_0000097
T2	1440-1444	Body_part	denotes	hand	http://purl.obolibrary.org/obo/MAT_0000091
T3	2048-2052	Body_part	denotes	hand	http://purl.obolibrary.org/obo/MAT_0000091

sentences

Id	Subject	Object	Predicate	Lexical cue
T1	0-105	Sentence	denotes	Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase.
T2	106-218	Sentence	denotes	UDP-GalNAc pyrophosphorylase (UDP-GalNAcPP; AGX1) catalyzes the synthesis of UDP-GalNAc from UTP and GalNAc-1-P.
T3	219-327	Sentence	denotes	The 475-amino acid protein (57 kDa protein) also synthesizes UDP-GlcNAc at about 25% the rate of UDP-GalNAc.
T4	328-511	Sentence	denotes	The cDNA for this enzyme, termed AGX1, was cloned in Escherichia coli, and expressed as an active enzyme that cross-reacted with antiserum against the original pig liver UDP-HexNAcPP.
T5	512-663	Sentence	denotes	In the present study, we incubated recombinant AGX1 with N(3)-UDP-[(32)P]GlcNAc and N(3)-UDP-[(32)P]GalNAc probes to label the nucleotide-binding site.
T6	664-869	Sentence	denotes	Proteolytic digestions of the labeled enzyme and analysis of the resulting peptides indicated that both photoprobes cross-linked to one 24-amino acid peptide located between residues Val(216) and Glu(240).
T7	870-1023	Sentence	denotes	Four amino acids in this peptide were found to be highly conserved among closely related enzymes, and each of these was individually modified to alanine.
T8	1024-1261	Sentence	denotes	Mutation of Gly(222) to Ala in the peptide almost completely eliminated UDP-GlcNAc and UDP-GalNAc synthesis, while mutation of Gly(224) to Ala, almost completely eliminated UDP-GalNAc synthesis, but UDP-GlcNAc was only diminished by 50%.
T9	1262-1426	Sentence	denotes	Both of these mutations also resulted in almost complete loss of the ability of the mutated proteins to cross-link N(3)-UDP-[(32)P]GlcNAc or N(3)-UDP-[(32)P]GalNAc.
T10	1427-1643	Sentence	denotes	On the other hand, mutations of either Pro(220) or Tyr(227) to Ala did not greatly affect enzymatic activity, although there was some reduction in the ability of these proteins to cross-link the photoaffinity probes.
T11	1644-1804	Sentence	denotes	We also mutated Gly(111) to Ala since this amino acid was reported to be necessary for catalysis (Mio, T., Yabe, T., Arisawa, M., and Yamada-Okabe, H. (1998) J.
T12	1805-1810	Sentence	denotes	Biol.
T13	1811-1816	Sentence	denotes	Chem.
T14	1817-1835	Sentence	denotes	273, 14392-14397).
T15	1836-2034	Sentence	denotes	The Gly(111) to Ala mutant lost all enzymatic activity, but interestingly enough, this mutant protein still cross-linked the radioactive N(3)-UDP-GlcNAc although not nearly as well as the wild type.
T16	2035-2197	Sentence	denotes	On the other hand, mutation of Arg(115) to Ala had no affect on enzymatic activity although it also reduced the amount of cross-linking of N(3)-UDP-[(32)P]GlcNAc.
T17	2198-2366	Sentence	denotes	These studies help to define essential amino acids at or near the nucleotide-binding site and the catalytic site, as well as peptides involved in binding and catalysis.

GlyCosmos15-Sentences

Id	Subject	Object	Predicate	Lexical cue
T1	0-105	Sentence	denotes	Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase.
T2	106-327	Sentence	denotes	UDP-GalNAc pyrophosphorylase (UDP-GalNAcPP; AGX1) catalyzes the synthesis of UDP-GalNAc from UTP and GalNAc-1-P. The 475-amino acid protein (57 kDa protein) also synthesizes UDP-GlcNAc at about 25% the rate of UDP-GalNAc.
T3	328-511	Sentence	denotes	The cDNA for this enzyme, termed AGX1, was cloned in Escherichia coli, and expressed as an active enzyme that cross-reacted with antiserum against the original pig liver UDP-HexNAcPP.
T4	512-663	Sentence	denotes	In the present study, we incubated recombinant AGX1 with N(3)-UDP-[(32)P]GlcNAc and N(3)-UDP-[(32)P]GalNAc probes to label the nucleotide-binding site.
T5	664-869	Sentence	denotes	Proteolytic digestions of the labeled enzyme and analysis of the resulting peptides indicated that both photoprobes cross-linked to one 24-amino acid peptide located between residues Val(216) and Glu(240).
T6	870-1023	Sentence	denotes	Four amino acids in this peptide were found to be highly conserved among closely related enzymes, and each of these was individually modified to alanine.
T7	1024-1261	Sentence	denotes	Mutation of Gly(222) to Ala in the peptide almost completely eliminated UDP-GlcNAc and UDP-GalNAc synthesis, while mutation of Gly(224) to Ala, almost completely eliminated UDP-GalNAc synthesis, but UDP-GlcNAc was only diminished by 50%.
T8	1262-1426	Sentence	denotes	Both of these mutations also resulted in almost complete loss of the ability of the mutated proteins to cross-link N(3)-UDP-[(32)P]GlcNAc or N(3)-UDP-[(32)P]GalNAc.
T9	1427-1643	Sentence	denotes	On the other hand, mutations of either Pro(220) or Tyr(227) to Ala did not greatly affect enzymatic activity, although there was some reduction in the ability of these proteins to cross-link the photoaffinity probes.
T10	1644-1835	Sentence	denotes	We also mutated Gly(111) to Ala since this amino acid was reported to be necessary for catalysis (Mio, T., Yabe, T., Arisawa, M., and Yamada-Okabe, H. (1998) J. Biol. Chem. 273, 14392-14397).
T11	1836-2034	Sentence	denotes	The Gly(111) to Ala mutant lost all enzymatic activity, but interestingly enough, this mutant protein still cross-linked the radioactive N(3)-UDP-GlcNAc although not nearly as well as the wild type.
T12	2035-2197	Sentence	denotes	On the other hand, mutation of Arg(115) to Ala had no affect on enzymatic activity although it also reduced the amount of cross-linking of N(3)-UDP-[(32)P]GlcNAc.
T13	2198-2366	Sentence	denotes	These studies help to define essential amino acids at or near the nucleotide-binding site and the catalytic site, as well as peptides involved in binding and catalysis.

GlyCosmos15-Glycan

Id	Subject	Object	Predicate	Lexical cue	image
T1	71-77	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2	79-85	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T3	110-116	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T4	187-193	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T5	207-213	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T6	284-290	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T7	320-326	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T8	585-591	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T9	612-618	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T10	1100-1106	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T11	1115-1121	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T12	1201-1207	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T13	1227-1233	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T14	1393-1399	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T15	1419-1425	Glycan	denotes	GalNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T16	1982-1988	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T17	2190-2196	Glycan	denotes	GlcNAc	https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

NCBITAXON

Id	Subject	Object	Predicate	Lexical cue	db_id
T1	381-397	OrganismTaxon	denotes	Escherichia coli	562

Anatomy-UBERON

Id	Subject	Object	Predicate	Lexical cue	uberon_id
T1	492-497	Body_part	denotes	liver	http://purl.obolibrary.org/obo/UBERON_0002107
T2	1440-1444	Body_part	denotes	hand	http://purl.obolibrary.org/obo/UBERON_0002398
T3	2048-2052	Body_part	denotes	hand	http://purl.obolibrary.org/obo/UBERON_0002398