PubMed:10591847 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10591847","sourcedb":"PubMed","sourceid":"10591847","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10591847","text":"Identification of salt-regulated genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803 by subtractive RNA hybridization.\nTo identify genes transcribed preferentially under salt stress, a subtractive RNA hybridization procedure was applied to the cyanobacterium Synechocystis sp. PCC 6803. The screening of a genomic library led to the identification of several RNA species that were more abundant in salt-stressed cells than in control cells. Salt-dependent transcription of the identified genes was verified in Northern blot experiments. In addition to the previously characterized genes cpn60 (encoding GroEL; a molecular chaperone) and isiA (encoding a chlorophyll-binding protein), genes encoding a protein of unknown function (slr0082) and a putative RNA helicase (slr0083) were identified as salt-regulated genes in Synechocystis. Genes slr0082 and slr0083, located at sites adjacent to each other on the Synechocystis chromosome, were transcribed from separate promoters and showed the most significant induction 1-3 h after salt shock. The salt-regulated promoters of these two genes were mapped. Genes cpn60, slr0082, and slr0083 were also found to be induced by a cold shock. The possible role of the identified gene products for salt adaptation of Synechocystis is discussed.","tracks":[{"project":"CyanoBase","denotations":[{"id":"T1","span":{"begin":661,"end":665},"obj":"protein"},{"id":"T2","span":{"begin":678,"end":705},"obj":"protein"},{"id":"T3","span":{"begin":754,"end":761},"obj":"protein"},{"id":"T4","span":{"begin":792,"end":799},"obj":"protein"},{"id":"T5","span":{"begin":865,"end":872},"obj":"protein"},{"id":"T6","span":{"begin":877,"end":884},"obj":"protein"},{"id":"T7","span":{"begin":1140,"end":1147},"obj":"protein"},{"id":"T8","span":{"begin":1153,"end":1160},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"CyanoBase"},{"subj":"T2","pred":"source","obj":"CyanoBase"},{"subj":"T3","pred":"source","obj":"CyanoBase"},{"subj":"T4","pred":"source","obj":"CyanoBase"},{"subj":"T5","pred":"source","obj":"CyanoBase"},{"subj":"T6","pred":"source","obj":"CyanoBase"},{"subj":"T7","pred":"source","obj":"CyanoBase"},{"subj":"T8","pred":"source","obj":"CyanoBase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"CyanoBase","color":"#ecb193","default":true}]}]}}