PubMed:10580653 JSONTXT

{"project":"GlyCosmos600-GlycoGenes2","denotations":[{"id":"T1","span":{"begin":53,"end":86},"obj":"https://acgg.asia/db/ggdb/info/gg077"},{"id":"T2","span":{"begin":143,"end":158},"obj":"https://acgg.asia/db/ggdb/info/gg077"},{"id":"T3","span":{"begin":152,"end":156},"obj":"https://acgg.asia/db/ggdb/info/gg184"},{"id":"T4","span":{"begin":170,"end":174},"obj":"https://acgg.asia/db/ggdb/info/gg184"},{"id":"T5","span":{"begin":312,"end":328},"obj":"https://acgg.asia/db/ggdb/info/gg077"},{"id":"T6","span":{"begin":622,"end":638},"obj":"https://acgg.asia/db/ggdb/info/gg077"},{"id":"T7","span":{"begin":882,"end":886},"obj":"https://acgg.asia/db/ggdb/info/gg184"},{"id":"T8","span":{"begin":960,"end":975},"obj":"https://acgg.asia/db/ggdb/info/gg077"},{"id":"T9","span":{"begin":969,"end":973},"obj":"https://acgg.asia/db/ggdb/info/gg184"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"GlyCosmos600-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":15,"end":34},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T2","span":{"begin":226,"end":245},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T3","span":{"begin":433,"end":452},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T4","span":{"begin":509,"end":528},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T5","span":{"begin":691,"end":710},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T6","span":{"begin":803,"end":822},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"GlyCosmos600-CLO","denotations":[{"id":"T1","span":{"begin":366,"end":376},"obj":"http://purl.obolibrary.org/obo/CLO_0008988"},{"id":"T2","span":{"begin":571,"end":575},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":15,"end":34},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":226,"end":245},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":433,"end":452},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":509,"end":528},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":691,"end":710},"obj":"Glycan_Motif"},{"id":"T6","span":{"begin":803,"end":822},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"},{"id":"A6","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":15,"end":34},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T2","span":{"begin":226,"end":245},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T3","span":{"begin":433,"end":452},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T4","span":{"begin":509,"end":528},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T5","span":{"begin":691,"end":710},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T6","span":{"begin":803,"end":822},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":94,"end":301},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":302,"end":544},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":545,"end":773},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":774,"end":976},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":301},"obj":"Sentence"},{"id":"T3","span":{"begin":302,"end":544},"obj":"Sentence"},{"id":"T4","span":{"begin":545,"end":773},"obj":"Sentence"},{"id":"T5","span":{"begin":774,"end":976},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

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in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"GlyCosmos600-FMA","denotations":[{"id":"T1","span":{"begin":152,"end":156},"obj":"Body_part"},{"id":"T2","span":{"begin":170,"end":174},"obj":"Body_part"},{"id":"T3","span":{"begin":226,"end":245},"obj":"Body_part"},{"id":"T4","span":{"begin":371,"end":376},"obj":"Body_part"},{"id":"T5","span":{"begin":509,"end":528},"obj":"Body_part"},{"id":"T6","span":{"begin":571,"end":575},"obj":"Body_part"},{"id":"T7","span":{"begin":691,"end":710},"obj":"Body_part"},{"id":"T8","span":{"begin":882,"end":886},"obj":"Body_part"},{"id":"T9","span":{"begin":969,"end":973},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"fma_id","subj":"T1","obj":"http://purl.org/sig/ont/fma/fma62820"},{"id":"A2","pred":"fma_id","subj":"T2","obj":"http://purl.org/sig/ont/fma/fma62820"},{"id":"A3","pred":"fma_id","subj":"T3","obj":"http://purl.org/sig/ont/fma/fma82787"},{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A5","pred":"fma_id","subj":"T5","obj":"http://purl.org/sig/ont/fma/fma82787"},{"id":"A6","pred":"fma_id","subj":"T6","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma82787"},{"id":"A8","pred":"fma_id","subj":"T8","obj":"http://purl.org/sig/ont/fma/fma62820"},{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma62820"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":306,"end":311},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"}],"text":"Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.\nUnlike classical beta-1,4-galactosyltransferase (beta-1,4-GalT I), beta-1,4-GalT V (formerly IV**) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95:472-477]. The human beta-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that beta-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 microM and 41 microM towards UDP-Gal, respectively. No significant inhibition of N-acetyllactosamine synthesis with alpha-lactalbumin was observed for beta-1,4-GalT V but the significant inhibition with alpha-lactalbumin was observed for beta-1,4-GalT I."}