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PubMed:10561460 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 721-728 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 721-728 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-169 Sentence denotes Glycosylation and proteolytic processing of 70 kDa C-terminal recombinant polypeptides of Plasmodium falciparum merozoite surface protein 1 expressed in mammalian cells.
TextSentencer_T2 170-484 Sentence denotes The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus.
TextSentencer_T3 485-601 Sentence denotes The polypeptides were studied with respect to the nature of glycosylation, localization, and proteolytic processing.
TextSentencer_T4 602-884 Sentence denotes The polypeptides derived from the cDNAs that contained the N-terminal signal peptide were modified with N -linked high mannose type structures and low levels of O -linked oligosaccharides, whereas the polypeptides from the cDNAs that lacked the signal peptide were not glycosylated.
TextSentencer_T5 885-1062 Sentence denotes The GPI anchor moiety is either absent or present at a very low level in the polypeptide expressed from the cDNA that contained both the signal peptide and GPI signal sequences.
TextSentencer_T6 1063-1236 Sentence denotes Together, these data establish that whereas the signal peptide of MSP-1 is functional, the GPI anchor signal is either nonfunctional or poorly functional in mammalian cells.
TextSentencer_T7 1237-1458 Sentence denotes The polypeptides expressed from the cDNAs that contained the signal peptide were proteolytically cleaved at their C-termini, whereas the polypeptides expressed from the cDNAs that lacked the signal peptide were uncleaved.
TextSentencer_T8 1459-1756 Sentence denotes While the polypeptide expressed from the cDNA containing both the signal peptide and GPI anchor signal was truncated by approximately 14 kDa at the C-terminus, the polypeptide derived from the cDNA with only the signal peptide was processed to remove approximately 6 kDa, also from the C-terminus.
TextSentencer_T9 1757-2099 Sentence denotes Furthermore, the polypeptides derived from cDNAs that lacked the signal peptide were exclusively localized intra-cellularly, the polypeptides from cDNAs that contained the signal peptide were predominantly intracellular, with low levels on the cell surface; none of the polypeptides was secreted into the culture medium to a detectable level.
TextSentencer_T10 2100-2297 Sentence denotes These results suggest that N -glycosylation alone is not sufficient for the efficient extracellular transport of the recombinant MSP-1 polypeptides through the secretory pathway in mammalian cells.
T1 0-169 Sentence denotes Glycosylation and proteolytic processing of 70 kDa C-terminal recombinant polypeptides of Plasmodium falciparum merozoite surface protein 1 expressed in mammalian cells.
T2 170-484 Sentence denotes The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus.
T3 485-601 Sentence denotes The polypeptides were studied with respect to the nature of glycosylation, localization, and proteolytic processing.
T4 602-884 Sentence denotes The polypeptides derived from the cDNAs that contained the N-terminal signal peptide were modified with N -linked high mannose type structures and low levels of O -linked oligosaccharides, whereas the polypeptides from the cDNAs that lacked the signal peptide were not glycosylated.
T5 885-1062 Sentence denotes The GPI anchor moiety is either absent or present at a very low level in the polypeptide expressed from the cDNA that contained both the signal peptide and GPI signal sequences.
T6 1063-1236 Sentence denotes Together, these data establish that whereas the signal peptide of MSP-1 is functional, the GPI anchor signal is either nonfunctional or poorly functional in mammalian cells.
T7 1237-1458 Sentence denotes The polypeptides expressed from the cDNAs that contained the signal peptide were proteolytically cleaved at their C-termini, whereas the polypeptides expressed from the cDNAs that lacked the signal peptide were uncleaved.
T8 1459-1756 Sentence denotes While the polypeptide expressed from the cDNA containing both the signal peptide and GPI anchor signal was truncated by approximately 14 kDa at the C-terminus, the polypeptide derived from the cDNA with only the signal peptide was processed to remove approximately 6 kDa, also from the C-terminus.
T9 1757-2099 Sentence denotes Furthermore, the polypeptides derived from cDNAs that lacked the signal peptide were exclusively localized intra-cellularly, the polypeptides from cDNAs that contained the signal peptide were predominantly intracellular, with low levels on the cell surface; none of the polypeptides was secreted into the culture medium to a detectable level.
T10 2100-2297 Sentence denotes These results suggest that N -glycosylation alone is not sufficient for the efficient extracellular transport of the recombinant MSP-1 polypeptides through the secretory pathway in mammalian cells.
T1 0-169 Sentence denotes Glycosylation and proteolytic processing of 70 kDa C-terminal recombinant polypeptides of Plasmodium falciparum merozoite surface protein 1 expressed in mammalian cells.
T2 170-484 Sentence denotes The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus.
T3 485-601 Sentence denotes The polypeptides were studied with respect to the nature of glycosylation, localization, and proteolytic processing.
T4 602-884 Sentence denotes The polypeptides derived from the cDNAs that contained the N-terminal signal peptide were modified with N -linked high mannose type structures and low levels of O -linked oligosaccharides, whereas the polypeptides from the cDNAs that lacked the signal peptide were not glycosylated.
T5 885-1062 Sentence denotes The GPI anchor moiety is either absent or present at a very low level in the polypeptide expressed from the cDNA that contained both the signal peptide and GPI signal sequences.
T6 1063-1236 Sentence denotes Together, these data establish that whereas the signal peptide of MSP-1 is functional, the GPI anchor signal is either nonfunctional or poorly functional in mammalian cells.
T7 1237-1458 Sentence denotes The polypeptides expressed from the cDNAs that contained the signal peptide were proteolytically cleaved at their C-termini, whereas the polypeptides expressed from the cDNAs that lacked the signal peptide were uncleaved.
T8 1459-1756 Sentence denotes While the polypeptide expressed from the cDNA containing both the signal peptide and GPI anchor signal was truncated by approximately 14 kDa at the C-terminus, the polypeptide derived from the cDNA with only the signal peptide was processed to remove approximately 6 kDa, also from the C-terminus.
T9 1757-2099 Sentence denotes Furthermore, the polypeptides derived from cDNAs that lacked the signal peptide were exclusively localized intra-cellularly, the polypeptides from cDNAs that contained the signal peptide were predominantly intracellular, with low levels on the cell surface; none of the polypeptides was secreted into the culture medium to a detectable level.
T10 2100-2297 Sentence denotes These results suggest that N -glycosylation alone is not sufficient for the efficient extracellular transport of the recombinant MSP-1 polypeptides through the secretory pathway in mammalian cells.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 721-728 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 90-111 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC770 denotes Plasmodium falciparum
_T2 225-246 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC770 denotes Plasmodium falciparum
_T3 469-483 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC566 denotes vaccinia virus

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 122-129 FMAID:50594 denotes surface
_T2 122-129 FMAID:146300 denotes surface
_T3 130-137 FMAID:165447 denotes protein
_T4 130-137 FMAID:67257 denotes protein
_T5 163-168 FMAID:169002 denotes cells
_T6 163-168 FMAID:68646 denotes cells
_T7 214-235 FMAID:167328 denotes portion of Plasmodium
_T8 214-235 FMAID:62970 denotes portion of Plasmodium
_T9 257-264 FMAID:146300 denotes surface
_T10 257-264 FMAID:50594 denotes surface
_T11 265-272 FMAID:165447 denotes protein
_T12 265-272 FMAID:67257 denotes protein
_T13 353-381 FMAID:196801 denotes glycosylphosphatidylinositol
_T14 353-381 FMAID:82806 denotes glycosylphosphatidylinositol
_T15 353-381 FMAID:199790 denotes glycosylphosphatidylinositol
_T16 353-381 FMAID:85436 denotes glycosylphosphatidylinositol
_T17 721-728 FMAID:196796 denotes mannose
_T18 721-728 FMAID:82801 denotes mannose
_T19 773-789 FMAID:82742 denotes oligosaccharides
_T20 773-789 FMAID:196731 denotes oligosaccharides
_T21 1230-1235 FMAID:169002 denotes cells
_T22 1230-1235 FMAID:68646 denotes cells
_T23 2001-2013 FMAID:212684 denotes cell surface
_T24 2001-2013 FMAID:200942 denotes cell surface
_T25 2006-2013 FMAID:146300 denotes surface
_T26 2006-2013 FMAID:50594 denotes surface
_T27 2291-2296 FMAID:169002 denotes cells
_T28 2291-2296 FMAID:68646 denotes cells

uniprot-human

Id Subject Object Predicate Lexical cue
T1 276-279 http://www.uniprot.org/uniprot/Q9BYE2 denotes MSP
T2 407-410 http://www.uniprot.org/uniprot/Q9BYE2 denotes MSP
T3 1129-1132 http://www.uniprot.org/uniprot/Q9BYE2 denotes MSP
T4 2229-2232 http://www.uniprot.org/uniprot/Q9BYE2 denotes MSP
T5 276-279 http://www.uniprot.org/uniprot/P26927 denotes MSP
T6 407-410 http://www.uniprot.org/uniprot/P26927 denotes MSP
T7 1129-1132 http://www.uniprot.org/uniprot/P26927 denotes MSP
T8 2229-2232 http://www.uniprot.org/uniprot/P26927 denotes MSP
T9 383-386 http://www.uniprot.org/uniprot/P06744 denotes GPI
T10 889-892 http://www.uniprot.org/uniprot/P06744 denotes GPI
T11 1041-1044 http://www.uniprot.org/uniprot/P06744 denotes GPI
T12 1154-1157 http://www.uniprot.org/uniprot/P06744 denotes GPI
T13 1544-1547 http://www.uniprot.org/uniprot/P06744 denotes GPI

FSU-PRGE

Id Subject Object Predicate Lexical cue
T1 112-139 protein denotes merozoite surface protein 1
T2 247-274 protein denotes merozoite surface protein 1
T3 276-281 protein denotes MSP-1
T4 407-412 protein denotes MSP-1
T5 1129-1134 protein denotes MSP-1
T6 2229-2234 protein denotes MSP-1

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 276-279 http://www.uniprot.org/uniprot/P26928 denotes MSP
T2 407-410 http://www.uniprot.org/uniprot/P26928 denotes MSP
T3 1129-1132 http://www.uniprot.org/uniprot/P26928 denotes MSP
T4 2229-2232 http://www.uniprot.org/uniprot/P26928 denotes MSP
T5 383-386 http://www.uniprot.org/uniprot/Q8C675 denotes GPI
T6 889-892 http://www.uniprot.org/uniprot/Q8C675 denotes GPI
T7 1041-1044 http://www.uniprot.org/uniprot/Q8C675 denotes GPI
T8 1154-1157 http://www.uniprot.org/uniprot/Q8C675 denotes GPI
T9 1544-1547 http://www.uniprot.org/uniprot/Q8C675 denotes GPI

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 90-100 http://purl.bioontology.org/ontology/NCBITAXON/5820 denotes Plasmodium
T2 90-100 http://purl.bioontology.org/ontology/NCBITAXON/5859 denotes Plasmodium
T3 90-111 http://purl.bioontology.org/ontology/NCBITAXON/5833 denotes Plasmodium falciparum
T4 163-168 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T5 225-235 http://purl.bioontology.org/ontology/NCBITAXON/5820 denotes Plasmodium
T6 225-235 http://purl.bioontology.org/ontology/NCBITAXON/5859 denotes Plasmodium
T7 225-246 http://purl.bioontology.org/ontology/NCBITAXON/5833 denotes Plasmodium falciparum
T8 478-483 http://purl.bioontology.org/ontology/NCBITAXON/184751 denotes virus
T9 1230-1235 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T10 2291-2296 http://purl.bioontology.org/ontology/STY/T025 denotes cells

GO-BP

Id Subject Object Predicate Lexical cue
T1 0-13 http://purl.obolibrary.org/obo/GO_0070085 denotes Glycosylation
T2 545-558 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T3 871-883 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylated
T4 314-320 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T5 388-394 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T6 672-678 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T7 847-853 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T8 1022-1028 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T9 1045-1051 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T10 1111-1117 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T11 1165-1171 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T12 1298-1304 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T13 1428-1434 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T14 1525-1531 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T15 1555-1561 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T16 1671-1677 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T17 1822-1828 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T18 1929-1935 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T19 560-572 http://purl.obolibrary.org/obo/GO_0051179 denotes localization
T20 1854-1863 http://purl.obolibrary.org/obo/GO_0051179 denotes localized
T21 1671-1699 http://purl.obolibrary.org/obo/GO_0006465 denotes signal peptide was processed
T22 1870-1880 http://purl.obolibrary.org/obo/GO_0007349 denotes cellularly
T23 2044-2052 http://purl.obolibrary.org/obo/GO_0046903 denotes secreted
T24 2186-2209 http://purl.obolibrary.org/obo/GO_0006858 denotes extracellular transport
T25 2200-2209 http://purl.obolibrary.org/obo/GO_0006810 denotes transport

GO-MF

Id Subject Object Predicate Lexical cue
T1 893-899 http://purl.obolibrary.org/obo/GO_0043495 denotes anchor
T2 1158-1164 http://purl.obolibrary.org/obo/GO_0043495 denotes anchor
T3 1548-1554 http://purl.obolibrary.org/obo/GO_0043495 denotes anchor

GO-CC

Id Subject Object Predicate Lexical cue
T1 163-168 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2 1230-1235 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3 442-446 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4 2001-2005 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5 1963-1976 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T6 2001-2013 http://purl.obolibrary.org/obo/GO_0009986 denotes cell surface
T7 2186-2199 http://purl.obolibrary.org/obo/GO_0005576 denotes extracellular

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 53-61 http://edamontology.org/topic_0749 denotes terminal
T2 130-137 http://edamontology.org/topic_0078 denotes protein
T3 130-149 http://edamontology.org/topic_0108 denotes protein 1 expressed
T4 130-149 http://edamontology.org/topic_0121 denotes protein 1 expressed
T5 174-179 http://edamontology.org/topic_3512 denotes cDNAs
T6 205-213 http://edamontology.org/topic_0749 denotes terminal
T7 265-272 http://edamontology.org/topic_0078 denotes protein
T8 305-313 http://edamontology.org/topic_0749 denotes terminal
T9 314-337 http://edamontology.org/topic_3512 denotes signal peptide sequence
T10 321-328 http://edamontology.org/topic_0154 denotes peptide
T11 329-337 http://edamontology.org/topic_0080 denotes sequence
T12 329-337 http://edamontology.org/topic_3168 denotes sequence
T13 344-352 http://edamontology.org/topic_0749 denotes terminal
T14 395-403 http://edamontology.org/topic_0080 denotes sequence
T15 395-403 http://edamontology.org/topic_3168 denotes sequence
T16 507-514 http://edamontology.org/topic_3678 denotes studied
T17 636-641 http://edamontology.org/topic_3512 denotes cDNAs
T18 663-671 http://edamontology.org/topic_0749 denotes terminal
T19 679-686 http://edamontology.org/topic_0154 denotes peptide
T20 825-830 http://edamontology.org/topic_3512 denotes cDNAs
T21 854-861 http://edamontology.org/topic_0154 denotes peptide
T22 993-997 http://edamontology.org/topic_3512 denotes cDNA
T23 1022-1061 http://edamontology.org/topic_3512 denotes signal peptide and GPI signal sequences
T24 1029-1036 http://edamontology.org/topic_0154 denotes peptide
T25 1052-1061 http://edamontology.org/topic_0080 denotes sequences
T26 1052-1061 http://edamontology.org/topic_3168 denotes sequences
T27 1118-1125 http://edamontology.org/topic_0154 denotes peptide
T28 1273-1278 http://edamontology.org/topic_3512 denotes cDNAs
T29 1305-1312 http://edamontology.org/topic_0154 denotes peptide
T30 1406-1411 http://edamontology.org/topic_3512 denotes cDNAs
T31 1435-1442 http://edamontology.org/topic_0154 denotes peptide
T32 1500-1504 http://edamontology.org/topic_3512 denotes cDNA
T33 1532-1539 http://edamontology.org/topic_0154 denotes peptide
T34 1652-1656 http://edamontology.org/topic_3512 denotes cDNA
T35 1678-1685 http://edamontology.org/topic_0154 denotes peptide
T36 1800-1805 http://edamontology.org/topic_3512 denotes cDNAs
T37 1829-1836 http://edamontology.org/topic_0154 denotes peptide
T38 1904-1909 http://edamontology.org/topic_3512 denotes cDNAs
T39 1936-1943 http://edamontology.org/topic_0154 denotes peptide
T40 2270-2277 http://edamontology.org/topic_0602 denotes pathway

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 30-40 http://edamontology.org/operation_2409 denotes processing
T2 30-40 http://edamontology.org/operation_0004 denotes processing
T3 130-137 http://edamontology.org/format_1208 denotes protein
T4 130-137 http://edamontology.org/data_1467 denotes protein
T5 265-272 http://edamontology.org/format_1208 denotes protein
T6 265-272 http://edamontology.org/data_1467 denotes protein
T7 321-328 http://edamontology.org/data_2906 denotes peptide
T8 329-337 http://edamontology.org/data_2044 denotes sequence
T9 329-337 http://edamontology.org/operation_3218 denotes sequence
T10 395-403 http://edamontology.org/data_2044 denotes sequence
T11 395-403 http://edamontology.org/operation_3218 denotes sequence
T12 590-600 http://edamontology.org/operation_2409 denotes processing
T13 590-600 http://edamontology.org/operation_0004 denotes processing
T14 679-686 http://edamontology.org/data_2906 denotes peptide
T15 734-744 http://edamontology.org/data_0883 denotes structures
T16 854-861 http://edamontology.org/data_2906 denotes peptide
T17 1029-1036 http://edamontology.org/data_2906 denotes peptide
T18 1052-1061 http://edamontology.org/operation_3218 denotes sequences
T19 1052-1061 http://edamontology.org/data_2044 denotes sequences
T20 1079-1083 http://edamontology.org/data_0006 denotes data
T21 1118-1125 http://edamontology.org/data_2906 denotes peptide
T22 1206-1219 http://edamontology.org/operation_0004 denotes functional in
T23 1305-1312 http://edamontology.org/data_2906 denotes peptide
T24 1435-1442 http://edamontology.org/data_2906 denotes peptide
T25 1532-1539 http://edamontology.org/data_2906 denotes peptide
T26 1678-1685 http://edamontology.org/data_2906 denotes peptide
T27 1690-1699 http://edamontology.org/operation_2409 denotes processed
T28 1690-1699 http://edamontology.org/operation_0004 denotes processed
T29 1829-1836 http://edamontology.org/data_2906 denotes peptide
T30 1936-1943 http://edamontology.org/data_2906 denotes peptide
T31 2082-2092 http://edamontology.org/operation_2423 denotes detectable
T32 2270-2277 http://edamontology.org/data_2600 denotes pathway

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 716-728 http://rdf.glycoinfo.org/glycan/G00028MO denotes high mannose
T2 889-899 http://rdf.glycoinfo.org/glycan/G00045MO denotes GPI anchor
T3 1154-1164 http://rdf.glycoinfo.org/glycan/G00045MO denotes GPI anchor
T4 1544-1554 http://rdf.glycoinfo.org/glycan/G00045MO denotes GPI anchor

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 469-477 Disease denotes vaccinia http://purl.obolibrary.org/obo/MONDO_0002595

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 90-111 OrganismTaxon denotes Plasmodium falciparum 5833
T2 225-246 OrganismTaxon denotes Plasmodium falciparum 5833
T3 469-483 OrganismTaxon denotes vaccinia virus 10245

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1864-1880 Body_part denotes intra-cellularly http://purl.obolibrary.org/obo/GO_0005622
T2 1963-1976 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T3 2186-2199 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576