PubMed:10556063 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10556063","sourcedb":"PubMed","sourceid":"10556063","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10556063","text":"Control of DAF-7 TGF-(alpha) expression and neuronal process development by a receptor tyrosine kinase KIN-8 in Caenorhabditis elegans.\nKIN-8 in C. elegans is highly homologous to human ROR-1 and 2 receptor tyrosine kinases of unknown functions. These kinases belong to a new subfamily related to the Trk subfamily. A kin-8 promoter::gfp fusion gene was expressed in ASI and many other neurons as well as in pharyngeal and head muscles. A kin-8 deletion mutant was isolated and showed constitutive dauer larva formation (Daf-c) phenotype: about half of the F(1) progeny became dauer larvae when they were cultivated on an old lawn of E. coli as food. Among the cells expressing kin-8::gfp, only ASI sensory neurons are known to express DAF-7 TGF-(beta), a key molecule preventing dauer larva formation. In the kin-8 deletion mutant, expression of daf-7::gfp in ASI was greatly reduced, dye-filling in ASI was specifically lost and ASI sensory processes did not completely extend into the amphid pore. The Daf-c phenotype was suppressed by daf-7 cDNA expression or a daf-3 null mutation. ASI-directed expression of kin-8 cDNA under the daf-7 promoter or expression by a heat shock promoter rescued the dye-filling defect, but not the Daf-c phenotype, of the kin-8 mutant. These results show that the kin-8 mutation causes the Daf-c phenotype through reduction of the daf-7 gene expression and that KIN-8 function is cell-autonomous for the dye-filling in ASI. KIN-8 is required for the process development of ASI, and also involved in promotion of daf-7 expression through a physiological or developmental function.","tracks":[{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":11,"end":16},"obj":"protein"},{"id":"T2","span":{"begin":17,"end":28},"obj":"protein"},{"id":"T3","span":{"begin":78,"end":102},"obj":"protein"},{"id":"T4","span":{"begin":103,"end":108},"obj":"protein"},{"id":"T5","span":{"begin":136,"end":141},"obj":"protein"},{"id":"T6","span":{"begin":186,"end":197},"obj":"protein"},{"id":"T7","span":{"begin":198,"end":223},"obj":"protein"},{"id":"T8","span":{"begin":252,"end":259},"obj":"protein"},{"id":"T9","span":{"begin":301,"end":314},"obj":"protein"},{"id":"T10","span":{"begin":318,"end":323},"obj":"protein"},{"id":"T11","span":{"begin":334,"end":337},"obj":"protein"},{"id":"T12","span":{"begin":439,"end":444},"obj":"protein"},{"id":"T13","span":{"begin":521,"end":524},"obj":"protein"},{"id":"T14","span":{"begin":678,"end":683},"obj":"protein"},{"id":"T15","span":{"begin":685,"end":688},"obj":"protein"},{"id":"T16","span":{"begin":736,"end":741},"obj":"protein"},{"id":"T17","span":{"begin":742,"end":752},"obj":"protein"},{"id":"T18","span":{"begin":810,"end":815},"obj":"protein"},{"id":"T19","span":{"begin":847,"end":852},"obj":"protein"},{"id":"T20","span":{"begin":854,"end":857},"obj":"protein"},{"id":"T21","span":{"begin":1005,"end":1008},"obj":"protein"},{"id":"T22","span":{"begin":1039,"end":1044},"obj":"protein"},{"id":"T23","span":{"begin":1066,"end":1071},"obj":"protein"},{"id":"T24","span":{"begin":1114,"end":1119},"obj":"protein"},{"id":"T25","span":{"begin":1135,"end":1140},"obj":"protein"},{"id":"T26","span":{"begin":1233,"end":1236},"obj":"protein"},{"id":"T27","span":{"begin":1257,"end":1262},"obj":"protein"},{"id":"T28","span":{"begin":1299,"end":1304},"obj":"protein"},{"id":"T29","span":{"begin":1325,"end":1328},"obj":"protein"},{"id":"T30","span":{"begin":1366,"end":1371},"obj":"protein"},{"id":"T31","span":{"begin":1397,"end":1402},"obj":"protein"},{"id":"T32","span":{"begin":1459,"end":1464},"obj":"protein"},{"id":"T33","span":{"begin":1547,"end":1552},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"FSU-PRGE"},{"subj":"T2","pred":"source","obj":"FSU-PRGE"},{"subj":"T3","pred":"source","obj":"FSU-PRGE"},{"subj":"T4","pred":"source","obj":"FSU-PRGE"},{"subj":"T5","pred":"source","obj":"FSU-PRGE"},{"subj":"T6","pred":"source","obj":"FSU-PRGE"},{"subj":"T7","pred":"source","obj":"FSU-PRGE"},{"subj":"T8","pred":"source","obj":"FSU-PRGE"},{"subj":"T9","pred":"source","obj":"FSU-PRGE"},{"subj":"T10","pred":"source","obj":"FSU-PRGE"},{"subj":"T11","pred":"source","obj":"FSU-PRGE"},{"subj":"T12","pred":"source","obj":"FSU-PRGE"},{"subj":"T13","pred":"source","obj":"FSU-PRGE"},{"subj":"T14","pred":"source","obj":"FSU-PRGE"},{"subj":"T15","pred":"source","obj":"FSU-PRGE"},{"subj":"T16","pred":"source","obj":"FSU-PRGE"},{"subj":"T17","pred":"source","obj":"FSU-PRGE"},{"subj":"T18","pred":"source","obj":"FSU-PRGE"},{"subj":"T19","pred":"source","obj":"FSU-PRGE"},{"subj":"T20","pred":"source","obj":"FSU-PRGE"},{"subj":"T21","pred":"source","obj":"FSU-PRGE"},{"subj":"T22","pred":"source","obj":"FSU-PRGE"},{"subj":"T23","pred":"source","obj":"FSU-PRGE"},{"subj":"T24","pred":"source","obj":"FSU-PRGE"},{"subj":"T25","pred":"source","obj":"FSU-PRGE"},{"subj":"T26","pred":"source","obj":"FSU-PRGE"},{"subj":"T27","pred":"source","obj":"FSU-PRGE"},{"subj":"T28","pred":"source","obj":"FSU-PRGE"},{"subj":"T29","pred":"source","obj":"FSU-PRGE"},{"subj":"T30","pred":"source","obj":"FSU-PRGE"},{"subj":"T31","pred":"source","obj":"FSU-PRGE"},{"subj":"T32","pred":"source","obj":"FSU-PRGE"},{"subj":"T33","pred":"source","obj":"FSU-PRGE"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"FSU-PRGE","color":"#9eec93","default":true}]}]}}