| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-58 |
Sentence |
denotes |
Accumulation of pre-apocytochrome f in a Synechocystis sp. |
| T2 |
59-111 |
Sentence |
denotes |
PCC 6803 mutant impaired in cytochrome c maturation. |
| T3 |
112-210 |
Sentence |
denotes |
Cytochrome c maturation involves heme transport and covalent attachment of heme to the apoprotein. |
| T4 |
211-438 |
Sentence |
denotes |
The 5' end of the ccsB gene, which is involved in the maturation process and resembles the ccs1 gene from Chlamydomonas reinhardtii, was replaced by a chloramphenicol resistance cartridge in the cyanobacterium Synechocystis sp. |
| T5 |
439-448 |
Sentence |
denotes |
PCC 6803. |
| T6 |
449-554 |
Sentence |
denotes |
The resulting Delta(M1-A24) mutant lacking the first 24 ccsB codons grew only under anaerobic conditions. |
| T7 |
555-717 |
Sentence |
denotes |
The mutant retained about 20% of the wild-type amount of processed cytochrome f with heme attached, apparently assembled in a functional cytochrome b(6)f complex. |
| T8 |
718-804 |
Sentence |
denotes |
Moreover, the mutant accumulated unprocessed apocytochrome f in its membrane fraction. |
| T9 |
805-895 |
Sentence |
denotes |
A pseudorevertant was isolated that regained the ability to grow under aerobic conditions. |
| T10 |
896-1151 |
Sentence |
denotes |
The locus of the second-site mutation was mapped to ccsB, and the mutation resulted in the formation of a new potential start codon in the intergenic region, between the chloramphenicol resistance marker and ccsB, in frame with the remaining part of ccsB. |
| T11 |
1152-1265 |
Sentence |
denotes |
In this pseudorevertant the amount of holocyt f increased, whereas that of unprocessed apocytochrome f decreased. |
| T12 |
1266-1384 |
Sentence |
denotes |
We suggest that the original deletion mutant Delta(M1-A24) expresses an N-terminally truncated version of the protein. |
| T13 |
1385-1616 |
Sentence |
denotes |
The stable accumulation of unprocessed apocytochrome f in membranes of the Delta(M1-A24) mutant may be explained by its association with truncated and only partially functional CcsB protein resulting in protection from degradation. |
| T14 |
1617-1688 |
Sentence |
denotes |
Our attempt to delete the first 244 codons of ccsB in Synechocystis sp. |
| T15 |
1689-1799 |
Sentence |
denotes |
PCC 6803 was not successful, suggesting that this would lead to a lack of functional cytochrome b(6)f complex. |
| T16 |
1800-1940 |
Sentence |
denotes |
The results suggest that the CcsB protein is an apocytochrome chaperone, which together with CcsA may constitute part of cytochrome c lyase. |
