PubMed:10528209 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10528209","sourcedb":"PubMed","sourceid":"10528209","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10528209","text":"A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages.\nC-type lectins serve multiple functions through recognizing carbohydrate chains. Here we report a novel C-type lectin, macrophage-inducible C-type lectin (Mincle), as a downstream target of NF-IL6 in macrophages. NF-IL6 belongs to the CCAAT/enhancer binding protein (C/EBP) of transcription factors and plays a crucial role in activated macrophages. However, what particular genes are regulated by NF-IL6 has been poorly defined in macrophages. Identification of downstream targets is required to elucidate the function of NF-IL6 in more detail. To identify downstream genes of NF-IL6, we screened a subtraction library constructed from wild-type and NF-IL6-deficient peritoneal macrophages and isolated Mincle that exhibits the highest homology to the members of group II C-type lectins. Mincle mRNA expression was strongly induced in response to several inflammatory stimuli, such as LPS, TNF-alpha, IL-6, and IFN-gamma in wild-type macrophages. In contrast, NF-IL6-deficient macrophages displayed a much lower level of Mincle mRNA induction following treatment with these inflammatory reagents. The mouse Mincle proximal promoter region contains an indispensable NF-IL6 binding element, demonstrating that Mincle is a direct target of NF-IL6. The Mincle gene locus was mapped at 0.6 centiMorgans proximal to CD4 on mouse chromosome 6.","tracks":[{"project":"2015-BEL-Sample-2","denotations":[{"id":"BEL:20067262","span":{"begin":1188,"end":1334},"obj":"tscript(p(MGI:Cebpb)) directlyIncreases r(MGI:Clec4e)"},{"id":"BEL:20067262","span":{"begin":1188,"end":1334},"obj":"tscript(p(MGI:Cebpb)) directlyIncreases r(MGI:Clec4e)"},{"id":"BEL:20063554","span":{"begin":879,"end":1036},"obj":"p(MGI:Tnf) increases r(MGI:Clec4e)"},{"id":"BEL:20053696","span":{"begin":879,"end":1036},"obj":"p(MGI:Ifng) increases r(MGI:Clec4e)"},{"id":"BEL:20055384","span":{"begin":879,"end":1036},"obj":"p(MGI:Il6) increases r(MGI:Clec4e)"}],"attributes":[{"subj":"BEL:20067262","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20067262","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20063554","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20053696","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20055384","pred":"source","obj":"2015-BEL-Sample-2"}]},{"project":"Lectin_function","denotations":[{"id":"T1","span":{"begin":22,"end":35},"obj":"GL_000114"},{"id":"T2","span":{"begin":22,"end":35},"obj":"GL_000115"},{"id":"T3","span":{"begin":29,"end":35},"obj":"GX_000027"},{"id":"T4","span":{"begin":29,"end":35},"obj":"GX_000045"},{"id":"T5","span":{"begin":90,"end":104},"obj":"GL_000114"},{"id":"T6","span":{"begin":90,"end":104},"obj":"GL_000115"},{"id":"T7","span":{"begin":194,"end":207},"obj":"GL_000114"},{"id":"T8","span":{"begin":194,"end":207},"obj":"GL_000115"},{"id":"T9","span":{"begin":201,"end":207},"obj":"GX_000027"},{"id":"T10","span":{"begin":201,"end":207},"obj":"GX_000045"},{"id":"T11","span":{"begin":230,"end":243},"obj":"GL_000114"},{"id":"T12","span":{"begin":230,"end":243},"obj":"GL_000115"},{"id":"T13","span":{"begin":237,"end":243},"obj":"GX_000027"},{"id":"T14","span":{"begin":237,"end":243},"obj":"GX_000045"},{"id":"T15","span":{"begin":863,"end":877},"obj":"GL_000114"},{"id":"T16","span":{"begin":863,"end":877},"obj":"GL_000115"},{"id":"T17","span":{"begin":946,"end":958},"obj":"FN_000024"},{"id":"T18","span":{"begin":1165,"end":1177},"obj":"FN_000024"}],"attributes":[{"subj":"T1","pred":"source","obj":"Lectin_function"},{"subj":"T2","pred":"source","obj":"Lectin_function"},{"subj":"T3","pred":"source","obj":"Lectin_function"},{"subj":"T4","pred":"source","obj":"Lectin_function"},{"subj":"T5","pred":"source","obj":"Lectin_function"},{"subj":"T6","pred":"source","obj":"Lectin_function"},{"subj":"T7","pred":"source","obj":"Lectin_function"},{"subj":"T8","pred":"source","obj":"Lectin_function"},{"subj":"T9","pred":"source","obj":"Lectin_function"},{"subj":"T10","pred":"source","obj":"Lectin_function"},{"subj":"T11","pred":"source","obj":"Lectin_function"},{"subj":"T12","pred":"source","obj":"Lectin_function"},{"subj":"T13","pred":"source","obj":"Lectin_function"},{"subj":"T14","pred":"source","obj":"Lectin_function"},{"subj":"T15","pred":"source","obj":"Lectin_function"},{"subj":"T16","pred":"source","obj":"Lectin_function"},{"subj":"T17","pred":"source","obj":"Lectin_function"},{"subj":"T18","pred":"source","obj":"Lectin_function"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2015-BEL-Sample-2","color":"#c793ec","default":true},{"id":"Lectin_function","color":"#93ecad"}]}]}}