| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-67 |
Sentence |
denotes |
Inactivation of the open reading frame slr0399 in Synechocystis sp. |
| T2 |
68-150 |
Sentence |
denotes |
PCC 6803 functionally complements mutations near the Q(A) niche of photosystem II. |
| T3 |
151-213 |
Sentence |
denotes |
A possible role of Slr0399 as a chaperone for quinone binding. |
| T4 |
214-235 |
Sentence |
denotes |
The Synechocystis sp. |
| T5 |
236-441 |
Sentence |
denotes |
PCC 6803 triple mutant D2R8 with V247M/A249T/M329I mutations in the D2 subunit of the photosystem II is impaired in Q(A) function, has an apparently mobile Q(A), and is unable to grow photoautotrophically. |
| T6 |
442-579 |
Sentence |
denotes |
Several photoautotrophic pseudorevertants of this mutant have been isolated, each of which retained the original psbDI mutations of D2R8. |
| T7 |
580-710 |
Sentence |
denotes |
Using a newly developed mapping technique, the site of the secondary mutations has been located in the open reading frame slr0399. |
| T8 |
711-963 |
Sentence |
denotes |
Two different nucleotide substitutions and a deletion of about 60% of slr0399 were each shown to restore photoautotrophy in different pseudorevertants of the mutant D2R8, suggesting that inactivation of Slr0399 leads to photoautotrophic growth in D2R8. |
| T9 |
964-1089 |
Sentence |
denotes |
Indeed, a targeted deletion of slr0399 restores photoautotrophy in D2R8 and in other psbDI mutants impaired in Q(A) function. |
| T10 |
1090-1329 |
Sentence |
denotes |
Slr0399 is similar to the hypothetical protein Ycf39, which is encoded in the cyanelle genome of Cyanophora paradoxa; in the chloroplast genomes of diatoms, dinoflagellates, and red algae; and in the nuclear genome of Arabidopsis thaliana. |
| T11 |
1330-1526 |
Sentence |
denotes |
Slr0399 and Ycf39 have a NAD(P)H binding motif near their N terminus and have some similarity to isoflavone reductase-like proteins and to a subunit of the eukaryotic NADH dehydrogenase complex I. |
| T12 |
1527-1577 |
Sentence |
denotes |
Deletion of slr0399 in wild type Synechocystis sp. |
| T13 |
1578-1726 |
Sentence |
denotes |
PCC 6803 has no significant phenotypic effects other than a decrease in thermotolerance under both photoautotrophic and photomixotrophic conditions. |
| T14 |
1727-1887 |
Sentence |
denotes |
We suggest that Slr0399 is a chaperone-like protein that aids in, but is not essential for, quinone insertion and protein folding around Q(A) in photosystem II. |
| T15 |
1888-2067 |
Sentence |
denotes |
Moreover, as the effects of Slr0399 are not limited to photosystem II, this protein may also be involved in assembly of quinones in other photosynthetic and respiratory complexes. |