| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-118 |
Sentence |
denotes |
Efficient adaptational demethylation of chemoreceptors requires the same enzyme-docking site as efficient methylation. |
| T2 |
119-367 |
Sentence |
denotes |
The mechanistic basis of sensory adaptation and gradient sensing in bacterial chemotaxis is reversible covalent modification of transmembrane chemoreceptors, methylation, and demethylation at specific glutamyl residues in their cytoplasmic domains. |
| T3 |
368-472 |
Sentence |
denotes |
These reactions are catalyzed by a dedicated methyltransferase CheR and a dedicated methylesterase CheB. |
| T4 |
473-593 |
Sentence |
denotes |
The esterase is also a deamidase that creates certain methyl-accepting glutamyls by hydrolysis of glutamine side chains. |
| T5 |
594-787 |
Sentence |
denotes |
We investigated the action of CheB and its activated form, phospho-CheB, on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor. |
| T6 |
788-884 |
Sentence |
denotes |
The deleted pentapeptide is conserved in several chemoreceptors in enteric and related bacteria. |
| T7 |
885-1065 |
Sentence |
denotes |
The truncated receptor was much less efficiently demethylated and deamidated than intact receptor, but essentially was unperturbed for kinase activation or transmembrane signaling. |
| T8 |
1066-1353 |
Sentence |
denotes |
CheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the methylesterase/deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock. |
| T9 |
1354-1555 |
Sentence |
denotes |
It is striking that the same pentapeptide serves as an activity-enhancing docking site for the methyltransferase CheR, the other enzyme involved in adaptational covalent modification of chemoreceptors. |
| T10 |
1556-1738 |
Sentence |
denotes |
A shared docking site raises the tantalizing possibility that relative rates of methylation and demethylation could be influenced by competition between the two enzymes at that site. |
