PubMed:10482545 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10482545","sourcedb":"PubMed","sourceid":"10482545","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10482545","text":"Induction of Bcl-x(L) expression by human T-cell leukemia virus type 1 Tax through NF-kappaB in apoptosis-resistant T-cell transfectants with Tax.\nHuman T-cell leukemia virus type 1 (HTLV-1) Tax is thought to play a pivotal role in immortalization of T cells. We have recently shown that the expression of Tax protected the mouse T-cell line CTLL-2 against apoptosis induced by interleukin-2 (IL-2) deprivation and converted its growth from being IL-2 dependent to being IL-2 independent. In this study, we demonstrate that constitutive expression of bcl-xl but not bcl-2, bcl-xs, bak, bad, or bax was associated with apoptosis resistance after IL-2 deprivation in CTLL-2 cells that expressed Tax. Transient-transfection assays showed that bcl-x promoter was transactivated by wild-type Tax. Similar effects were observed in mutant Tax retaining transactivating ability through NF-kappaB. Deletion or substitution of a putative NF-kappaB binding site identified in the bcl-x promoter significantly decreased Tax-induced transactivation. This NF-kappaB-like element was able to form a complex with NF-kappaB family proteins in vitro. Furthermore, Tax-induced transactivation of the bcl-x promoter was also diminished by the mutant IkappaBalpha, which specifically inhibits NF-kappaB activity. Our findings suggest that constitutive expression of Bcl-x(L) induced by Tax through the NF-kappaB pathway contributes to the inhibition of apoptosis in CTLL-2 cells after IL-2 deprivation.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C3","span":{"begin":36,"end":74},"obj":"NP"},{"id":"C28","span":{"begin":83,"end":92},"obj":"NP"},{"id":"C42","span":{"begin":96,"end":106},"obj":"NP"},{"id":"C29","span":{"begin":142,"end":145},"obj":"NP"},{"id":"C2","span":{"begin":147,"end":194},"obj":"NP"},{"id":"C7","span":{"begin":306,"end":309},"obj":"NP"},{"id":"C11","span":{"begin":320,"end":348},"obj":"NP"},{"id":"C43","span":{"begin":357,"end":366},"obj":"NP"},{"id":"C32","span":{"begin":378,"end":410},"obj":"NP"},{"id":"C31","span":{"begin":425,"end":428},"obj":"NP"},{"id":"C10","span":{"begin":645,"end":661},"obj":"NP"},{"id":"C12","span":{"begin":665,"end":677},"obj":"NP"},{"id":"C34","span":{"begin":678,"end":682},"obj":"NP"},{"id":"C13","span":{"begin":693,"end":696},"obj":"NP"},{"id":"C35","span":{"begin":740,"end":754},"obj":"NP"},{"id":"C15","span":{"begin":878,"end":887},"obj":"NP"},{"id":"C36","span":{"begin":965,"end":983},"obj":"NP"},{"id":"C38","span":{"begin":1008,"end":1012},"obj":"NP"},{"id":"C22","span":{"begin":1008,"end":1035},"obj":"NP"},{"id":"C19","span":{"begin":1042,"end":1052},"obj":"NP"},{"id":"C39","span":{"begin":1146,"end":1150},"obj":"NP"},{"id":"C37","span":{"begin":1177,"end":1195},"obj":"NP"},{"id":"C23","span":{"begin":1146,"end":1195},"obj":"NP"},{"id":"C40","span":{"begin":1219,"end":1242},"obj":"NP"},{"id":"C41","span":{"begin":1244,"end":1249},"obj":"NP"},{"id":"C24","span":{"begin":1365,"end":1368},"obj":"NP"},{"id":"C44","span":{"begin":1432,"end":1441},"obj":"NP"},{"id":"C26","span":{"begin":1445,"end":1457},"obj":"NP"},{"id":"C27","span":{"begin":1464,"end":1480},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C3"},{"id":"R2","pred":"coref-ident","subj":"C7","obj":"C29"},{"id":"R3","pred":"coref-ident","subj":"C43","obj":"C42"},{"id":"R4","pred":"coref-pron","subj":"C31","obj":"C11"},{"id":"R5","pred":"coref-ident","subj":"C10","obj":"C32"},{"id":"R6","pred":"coref-ident","subj":"C12","obj":"C11"},{"id":"R7","pred":"coref-relat","subj":"C34","obj":"C12"},{"id":"R8","pred":"coref-ident","subj":"C13","obj":"C7"},{"id":"R9","pred":"coref-ident","subj":"C15","obj":"C28"},{"id":"R10","pred":"coref-ident","subj":"C36","obj":"C35"},{"id":"R11","pred":"coref-xxx","subj":"C22","obj":"C13"},{"id":"R12","pred":"coref-ident","subj":"C19","obj":"C15"},{"id":"R13","pred":"coref-other","subj":"C39","obj":"C38"},{"id":"R14","pred":"coref-ident","subj":"C37","obj":"C36"},{"id":"R15","pred":"coref-ident","subj":"C23","obj":"C22"},{"id":"R16","pred":"coref-relat","subj":"C41","obj":"C40"},{"id":"R17","pred":"coref-ident","subj":"C24","obj":"C23"},{"id":"R18","pred":"coref-ident","subj":"C44","obj":"C43"},{"id":"R19","pred":"coref-ident","subj":"C26","obj":"C12"},{"id":"R20","pred":"coref-ident","subj":"C27","obj":"C10"}],"attributes":[{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C28","pred":"source","obj":"genia-medco-coref"},{"subj":"C42","pred":"source","obj":"genia-medco-coref"},{"subj":"C29","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C43","pred":"source","obj":"genia-medco-coref"},{"subj":"C32","pred":"source","obj":"genia-medco-coref"},{"subj":"C31","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C34","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C35","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C36","pred":"source","obj":"genia-medco-coref"},{"subj":"C38","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C39","pred":"source","obj":"genia-medco-coref"},{"subj":"C37","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C40","pred":"source","obj":"genia-medco-coref"},{"subj":"C41","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C44","pred":"source","obj":"genia-medco-coref"},{"subj":"C26","pred":"source","obj":"genia-medco-coref"},{"subj":"C27","pred":"source","obj":"genia-medco-coref"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":160,"end":168},"obj":"HP_0001909"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":13,"end":21},"obj":"protein"},{"id":"T2","span":{"begin":36,"end":74},"obj":"protein"},{"id":"T3","span":{"begin":83,"end":92},"obj":"protein"},{"id":"T4","span":{"begin":96,"end":136},"obj":"cell_line"},{"id":"T5","span":{"begin":142,"end":145},"obj":"protein"},{"id":"T6","span":{"begin":191,"end":194},"obj":"protein"},{"id":"T7","span":{"begin":251,"end":258},"obj":"cell_type"},{"id":"T8","span":{"begin":306,"end":309},"obj":"protein"},{"id":"T9","span":{"begin":324,"end":348},"obj":"cell_line"},{"id":"T10","span":{"begin":378,"end":391},"obj":"protein"},{"id":"T11","span":{"begin":393,"end":397},"obj":"protein"},{"id":"T12","span":{"begin":447,"end":451},"obj":"protein"},{"id":"T13","span":{"begin":471,"end":475},"obj":"protein"},{"id":"T14","span":{"begin":551,"end":557},"obj":"protein"},{"id":"T15","span":{"begin":566,"end":571},"obj":"protein"},{"id":"T16","span":{"begin":573,"end":579},"obj":"protein"},{"id":"T17","span":{"begin":581,"end":584},"obj":"protein"},{"id":"T18","span":{"begin":586,"end":589},"obj":"protein"},{"id":"T19","span":{"begin":594,"end":597},"obj":"protein"},{"id":"T20","span":{"begin":645,"end":649},"obj":"protein"},{"id":"T21","span":{"begin":665,"end":677},"obj":"cell_line"},{"id":"T22","span":{"begin":693,"end":696},"obj":"protein"},{"id":"T23","span":{"begin":740,"end":754},"obj":"DNA"},{"id":"T24","span":{"begin":777,"end":790},"obj":"protein"},{"id":"T25","span":{"begin":832,"end":835},"obj":"protein"},{"id":"T26","span":{"begin":878,"end":887},"obj":"protein"},{"id":"T27","span":{"begin":919,"end":950},"obj":"protein"},{"id":"T28","span":{"begin":969,"end":983},"obj":"DNA"},{"id":"T29","span":{"begin":1008,"end":1011},"obj":"protein"},{"id":"T30","span":{"begin":1042,"end":1064},"obj":"DNA"},{"id":"T31","span":{"begin":1097,"end":1122},"obj":"protein"},{"id":"T32","span":{"begin":1146,"end":1149},"obj":"protein"},{"id":"T33","span":{"begin":1181,"end":1195},"obj":"DNA"},{"id":"T34","span":{"begin":1223,"end":1242},"obj":"protein"},{"id":"T35","span":{"begin":1272,"end":1281},"obj":"protein"},{"id":"T36","span":{"begin":1345,"end":1353},"obj":"protein"},{"id":"T37","span":{"begin":1365,"end":1368},"obj":"protein"},{"id":"T38","span":{"begin":1381,"end":1390},"obj":"protein"},{"id":"T39","span":{"begin":1445,"end":1457},"obj":"cell_line"},{"id":"T40","span":{"begin":1464,"end":1468},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"},{"subj":"T32","pred":"source","obj":"jnlpba-st-training"},{"subj":"T33","pred":"source","obj":"jnlpba-st-training"},{"subj":"T34","pred":"source","obj":"jnlpba-st-training"},{"subj":"T35","pred":"source","obj":"jnlpba-st-training"},{"subj":"T36","pred":"source","obj":"jnlpba-st-training"},{"subj":"T37","pred":"source","obj":"jnlpba-st-training"},{"subj":"T38","pred":"source","obj":"jnlpba-st-training"},{"subj":"T39","pred":"source","obj":"jnlpba-st-training"},{"subj":"T40","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":146},"obj":"Sentence"},{"id":"S2","span":{"begin":147,"end":259},"obj":"Sentence"},{"id":"S3","span":{"begin":260,"end":488},"obj":"Sentence"},{"id":"S4","span":{"begin":489,"end":697},"obj":"Sentence"},{"id":"S5","span":{"begin":698,"end":791},"obj":"Sentence"},{"id":"S6","span":{"begin":792,"end":888},"obj":"Sentence"},{"id":"S7","span":{"begin":889,"end":1036},"obj":"Sentence"},{"id":"S8","span":{"begin":1037,"end":1132},"obj":"Sentence"},{"id":"S9","span":{"begin":1133,"end":1291},"obj":"Sentence"},{"id":"S10","span":{"begin":1292,"end":1481},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S10","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":13,"end":21},"obj":"protein_molecule"},{"id":"T2","span":{"begin":36,"end":70},"obj":"virus"},{"id":"T3","span":{"begin":83,"end":92},"obj":"protein_complex"},{"id":"T4","span":{"begin":96,"end":136},"obj":"cell_line"},{"id":"T5","span":{"begin":142,"end":145},"obj":"protein_molecule"},{"id":"T6","span":{"begin":147,"end":181},"obj":"virus"},{"id":"T7","span":{"begin":183,"end":189},"obj":"virus"},{"id":"T8","span":{"begin":191,"end":194},"obj":"protein_molecule"},{"id":"T9","span":{"begin":251,"end":258},"obj":"cell_type"},{"id":"T10","span":{"begin":306,"end":309},"obj":"protein_molecule"},{"id":"T11","span":{"begin":324,"end":348},"obj":"cell_line"},{"id":"T12","span":{"begin":378,"end":391},"obj":"protein_molecule"},{"id":"T13","span":{"begin":393,"end":397},"obj":"protein_molecule"},{"id":"T14","span":{"begin":447,"end":451},"obj":"protein_molecule"},{"id":"T15","span":{"begin":471,"end":475},"obj":"protein_molecule"},{"id":"T16","span":{"begin":551,"end":557},"obj":"protein_molecule"},{"id":"T17","span":{"begin":566,"end":571},"obj":"protein_molecule"},{"id":"T18","span":{"begin":573,"end":579},"obj":"protein_molecule"},{"id":"T19","span":{"begin":581,"end":584},"obj":"protein_molecule"},{"id":"T20","span":{"begin":586,"end":589},"obj":"protein_molecule"},{"id":"T21","span":{"begin":594,"end":597},"obj":"protein_molecule"},{"id":"T22","span":{"begin":618,"end":638},"obj":"other_name"},{"id":"T23","span":{"begin":645,"end":649},"obj":"protein_molecule"},{"id":"T24","span":{"begin":665,"end":677},"obj":"cell_line"},{"id":"T25","span":{"begin":693,"end":696},"obj":"protein_molecule"},{"id":"T26","span":{"begin":698,"end":727},"obj":"other_name"},{"id":"T27","span":{"begin":740,"end":754},"obj":"DNA_domain_or_region"},{"id":"T28","span":{"begin":777,"end":786},"obj":"protein_molecule"},{"id":"T29","span":{"begin":787,"end":790},"obj":"protein_molecule"},{"id":"T30","span":{"begin":832,"end":835},"obj":"protein_molecule"},{"id":"T31","span":{"begin":878,"end":887},"obj":"protein_complex"},{"id":"T32","span":{"begin":919,"end":927},"obj":"protein_domain_or_region"},{"id":"T33","span":{"begin":928,"end":937},"obj":"protein_complex"},{"id":"T34","span":{"begin":969,"end":983},"obj":"DNA_domain_or_region"},{"id":"T35","span":{"begin":1008,"end":1011},"obj":"protein_molecule"},{"id":"T36","span":{"begin":1042,"end":1051},"obj":"protein_complex"},{"id":"T37","span":{"begin":1097,"end":1106},"obj":"protein_complex"},{"id":"T38","span":{"begin":1146,"end":1149},"obj":"protein_molecule"},{"id":"T39","span":{"begin":1181,"end":1195},"obj":"DNA_domain_or_region"},{"id":"T40","span":{"begin":1223,"end":1242},"obj":"protein_molecule"},{"id":"T41","span":{"begin":1272,"end":1281},"obj":"protein_molecule"},{"id":"T42","span":{"begin":1318,"end":1341},"obj":"other_name"},{"id":"T43","span":{"begin":1345,"end":1353},"obj":"protein_molecule"},{"id":"T44","span":{"begin":1365,"end":1368},"obj":"protein_molecule"},{"id":"T45","span":{"begin":1381,"end":1390},"obj":"protein_complex"},{"id":"T46","span":{"begin":1445,"end":1457},"obj":"cell_line"},{"id":"T47","span":{"begin":1464,"end":1468},"obj":"protein_molecule"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"},{"subj":"T42","pred":"source","obj":"GENIAcorpus"},{"subj":"T43","pred":"source","obj":"GENIAcorpus"},{"subj":"T44","pred":"source","obj":"GENIAcorpus"},{"subj":"T45","pred":"source","obj":"GENIAcorpus"},{"subj":"T46","pred":"source","obj":"GENIAcorpus"},{"subj":"T47","pred":"source","obj":"GENIAcorpus"}]},{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":42,"end":57},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":153,"end":168},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":183,"end":189},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0005525"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0005525"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0005801"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#eccf93","default":true},{"id":"PubmedHPO","color":"#b593ec"},{"id":"jnlpba-st-training","color":"#93ec9b"},{"id":"pubmed-sentences-benchmark","color":"#ec93a5"},{"id":"GENIAcorpus","color":"#93bfec"},{"id":"PMID_GLOBAL","color":"#d9ec93"}]}]}}