| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-133 |
Sentence |
denotes |
Cobalt-dependent transcriptional switching by a dual-effector MerR-like protein regulates a cobalt-exporting variant CPx-type ATPase. |
| T2 |
134-225 |
Sentence |
denotes |
CoaR associates with and confers cobalt-dependent activation of the coaT operator-promoter. |
| T3 |
226-484 |
Sentence |
denotes |
A CoaR mutant (Ser-Asn-Ser) in a carboxyl-terminal Cys-His-Cys motif bound the coaT operator-promoter but did not activate expression in response to cobalt, implicating thiolate and/or imidazole ligands at these residues in an allosteric cobalt binding site. |
| T4 |
485-701 |
Sentence |
denotes |
Deletion of 1 or 2 nucleotides from between near consensus, but with aberrant (20 base pairs) spacing, -10 and -35 elements enhanced expression from the coaT operator-promoter but abolished activation by cobalt-CoaR. |
| T5 |
702-830 |
Sentence |
denotes |
It is inferred that cobalt effects a transition in CoaR that underwinds the coaT operator-promoter to realign promoter elements. |
| T6 |
831-903 |
Sentence |
denotes |
In the absence of cobalt, CoaR represses expression (approximately 50%). |
| T7 |
904-1068 |
Sentence |
denotes |
CoaR is a fusion of ancestral MerR (mercury-responsive transcriptional activator)- and precorrin isomerase (enzyme of vitamin B(12) biosynthesis)-related sequences. |
| T8 |
1069-1282 |
Sentence |
denotes |
Expression from the coaT operator-promoter was enhanced in a partial mutant of cbiE (encoding an enzyme preceding precorrin isomerase in B(12) biosynthesis), revealing that this pathway "inhibits" coaT expression. |
| T9 |
1283-1457 |
Sentence |
denotes |
Disruption of coaT reduced cobalt tolerance and increased cytoplasmic (57)Co accumulation. coaT-mediated restoration of cobalt tolerance has been used as a selectable marker. |
