PubMed:10446291
Annnotations
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 0-9 | Glycan | denotes | Melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T2 | 149-158 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T3 | 506-515 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T4 | 549-558 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T5 | 751-760 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T6 | 950-959 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T7 | 1229-1238 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T8 | 1431-1440 | Glycan | denotes | Melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T9 | 1891-1900 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 0-9 | Glycan | denotes | Melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T2 | 149-158 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T3 | 506-515 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T4 | 549-558 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T5 | 751-760 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T6 | 950-959 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T7 | 1229-1238 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T8 | 1431-1440 | Glycan | denotes | Melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
| T9 | 1891-1900 | Glycan | denotes | melibiose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66481II |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 114-118 | Body_part | denotes | face | http://purl.obolibrary.org/obo/UBERON_0001456 |
| T2 | 136-141 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T3 | 346-354 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/UBERON_0000094 |
| T4 | 378-383 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T5 | 675-680 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T6 | 1572-1577 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T7 | 1837-1841 | Body_part | denotes | axis | http://purl.obolibrary.org/obo/UBERON_0001093 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 21-37 | Organism | denotes | Escherichia coli | 562 |
| T2 | 172-188 | Organism | denotes | Escherichia coli | 562 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-144 | Sentence | denotes | Melibiose carrier of Escherichia coli: use of cysteine mutagenesis to identify the amino acids on the hydrophilic face of transmembrane helix 2. |
| T2 | 145-223 | Sentence | denotes | The melibiose carrier from Escherichia coli is a galactoside-cation symporter. |
| T3 | 224-363 | Sentence | denotes | Based on both experimental evidence and hydropathy analysis, 12 transmembrane helices have been assigned to this integral membrane protein. |
| T4 | 364-516 | Sentence | denotes | Transmembrane helix 2 contains several charged and polar amino acids that have been shown to be essential for the cation-coupled transport of melibiose. |
| T5 | 517-683 | Sentence | denotes | Starting with the cysteine-less melibiose carrier, we have individually substituted cysteine for amino acids 39-66, which includes the proposed transmembrane helix 2. |
| T6 | 684-1027 | Sentence | denotes | In the resulting derivative carriers, we measured the transport of melibiose, determined the effect of the hydrophilic sulfhydryl reagent, p-chloromercuribenzenesulfonic acid (PCMBS), on transport in intact cells and inside out vesicles, and examined the ability of melibiose to protect the carrier from inactivation by the sulfhydryl reagent. |
| T7 | 1028-1212 | Sentence | denotes | We found a set of seven positions in which the reaction with the sulfhydryl reagent caused partial or complete loss of carrier function measured in intact cells or inside-out vesicles. |
| T8 | 1213-1298 | Sentence | denotes | The presence of melibiose protected five of these positions from reaction with PCMBS. |
| T9 | 1299-1430 | Sentence | denotes | The reaction of two additional positions with PCMBS resulted in the partial loss of transport function only in inside-out vesicles. |
| T10 | 1431-1502 | Sentence | denotes | Melibiose protected these two positions from reaction with the reagent. |
| T11 | 1503-1681 | Sentence | denotes | Together, the PCMBS-sensitive sites and charged residues assigned to helix 2 form a cluster of amino acids that map in three rows with each row comprised of every fourth residue. |
| T12 | 1682-1842 | Sentence | denotes | This is the pattern expected of residues that are part of an alpha-helical structure and thus the rows are tilted at an angle of 25 degrees to the helical axis. |
| T13 | 1843-1947 | Sentence | denotes | We suggest that these residues line the path of melibiose and its associated cation through the carrier. |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 114-118 | Body_part | denotes | face | FMA:24728 |
| T2 | 136-141 | Body_part | denotes | helix | FMA:60992 |
| T3 | 378-383 | Body_part | denotes | helix | FMA:60992 |
| T4 | 675-680 | Body_part | denotes | helix | FMA:60992 |
| T5 | 1572-1577 | Body_part | denotes | helix | FMA:60992 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 21-37 | OrganismTaxon | denotes | Escherichia coli | 562 |
| T2 | 172-188 | OrganismTaxon | denotes | Escherichia coli | 562 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 114-118 | Body_part | denotes | face | http://purl.obolibrary.org/obo/UBERON_0001456 |
| T2 | 122-135 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T3 | 136-141 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T4 | 288-301 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T5 | 346-354 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |
| T8 | 364-377 | Body_part | denotes | Transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T9 | 378-383 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T10 | 661-674 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T11 | 675-680 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T12 | 1572-1577 | Body_part | denotes | helix | http://purl.obolibrary.org/obo/UBERON_0002488 |
| T13 | 1837-1841 | Body_part | denotes | axis | http://purl.obolibrary.org/obo/UBERON_0001093 |