| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-146 |
Sentence |
denotes |
In vitro random mutagenesis of the D1 protein of the photosystem II reaction center confers phototolerance on the cyanobacterium Synechocystis sp. |
| T2 |
147-156 |
Sentence |
denotes |
PCC 6803. |
| T3 |
157-294 |
Sentence |
denotes |
The D1 protein of the photosystem II reaction center is thought to be the most light-sensitive component of the photosynthetic machinery. |
| T4 |
295-501 |
Sentence |
denotes |
To understand the mechanisms underlying the light sensitivity of D1, we performed in vitro random mutagenesis of the psbA gene that codes for D1, transformed the unicellular cyanobacterium Synechocystis sp. |
| T5 |
502-615 |
Sentence |
denotes |
PCC 6803 with mutated psbA, and selected phototolerant transformants that did not bleach in high intensity light. |
| T6 |
616-831 |
Sentence |
denotes |
A region of psbA2 coding for 178 amino acids of the carboxyl-terminal portion of the peptide was subjected to random mutagenesis by low fidelity polymerase chain reaction amplification or by hydroxylamine treatment. |
| T7 |
832-907 |
Sentence |
denotes |
This region contains the binding sites for Q(B), D2 (through Fe), and P680. |
| T8 |
908-1088 |
Sentence |
denotes |
Eighteen phototolerant mutants with single and multiple amino acid substitutions were selected from a half million transformants exposed to white light at 320 micromol m(-2) s(-1). |
| T9 |
1089-1179 |
Sentence |
denotes |
A strain transformed with non-mutagenized psbA2 became bleached under the same conditions. |
| T10 |
1180-1339 |
Sentence |
denotes |
Site-directed mutagenesis has confirmed that one or more substitutions of amino acids at residues 234, 254, 260, 267, 322, 326, and 328 confers phototolerance. |
| T11 |
1340-1424 |
Sentence |
denotes |
The rate of degradation of D1 protein was not appreciably affected by the mutations. |
| T12 |
1425-1597 |
Sentence |
denotes |
Reduced bleaching of mutant cyanobacterial cells may result from continued buildup of photosynthetic pigment systems caused by changes in redox signals originating from D1. |