> top > docs > PubMed:10428853 > annotations

PubMed:10428853 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-64 Sentence denotes Tissue-specific regulation of the ecto-5'-nucleotidase promoter.
T2 65-162 Sentence denotes Role of the camp response element site in mediating repression by the upstream regulatory region.
T3 163-440 Sentence denotes We have isolated the 5' region of the ecto-5'-nucleotidase (low K(m) 5'-NT) gene and established that a 969-base pair (bp) fragment confers cell-specific expression of a CAT reporter gene that correlates with the expression of endogenous ecto-5'-NT mRNA and enzymatic activity.
T4 441-635 Sentence denotes A 768-bp upstream negative regulatory region has been identified that conferred lymphocyte-specific negative regulation in a heterologous system with a 244-bp deoxycytidine kinase core promoter.
T5 636-894 Sentence denotes DNase I footprinting identified several protected areas including Sp1, Sp1/AP-2, and cAMP response element (CRE) binding sites within the 201-bp core promoter region and Sp1, NRE-2a, TCF-1/LEF-1, and Sp1/NF-AT binding sites in the upstream regulatory region.
T6 895-1109 Sentence denotes Whereas the CRE site was essential in mediating the negative activity of the upstream regulatory region in Jurkat but not in HeLa cells, mutation of the Sp1/AP-2 site decreased promoter activity in both cell lines.
T7 1110-1240 Sentence denotes Electrophoretic mobility shift assay analysis of proteins binding to the CRE site identified both ATF-1 and ATF-2 in Jurkat cells.
T8 1241-1418 Sentence denotes Finally, phorbol 12-myristate 13-acetate increased the activity of both the core and the 969-bp promoter fragments, and this increase was abrogated by mutations at the CRE site.
T9 1419-1628 Sentence denotes In summary, we have identified a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter that requires the presence of a functional CRE site within the basal promoter for its suppressive activity.

jnlpba-st-training

Id Subject Object Predicate Lexical cue
T1 34-63 DNA denotes ecto-5'-nucleotidase promoter
T2 77-103 DNA denotes camp response element site
T3 135-161 DNA denotes upstream regulatory region
T4 184-193 DNA denotes 5' region
T5 201-243 DNA denotes ecto-5'-nucleotidase (low K(m) 5'-NT) gene
T6 267-294 DNA denotes 969-base pair (bp) fragment
T7 333-350 DNA denotes CAT reporter gene
T8 390-416 RNA denotes endogenous ecto-5'-NT mRNA
T9 443-485 DNA denotes 768-bp upstream negative regulatory region
T10 593-634 DNA denotes 244-bp deoxycytidine kinase core promoter
T11 636-643 protein denotes DNase I
T12 702-762 DNA denotes Sp1, Sp1/AP-2, and cAMP response element (CRE) binding sites
T13 806-859 DNA denotes Sp1, NRE-2a, TCF-1/LEF-1, and Sp1/NF-AT binding sites
T14 867-893 DNA denotes upstream regulatory region
T15 907-915 DNA denotes CRE site
T16 972-998 DNA denotes upstream regulatory region
T17 1002-1008 cell_line denotes Jurkat
T18 1020-1030 cell_line denotes HeLa cells
T19 1048-1061 DNA denotes Sp1/AP-2 site
T20 1183-1191 DNA denotes CRE site
T21 1208-1213 protein denotes ATF-1
T22 1218-1223 protein denotes ATF-2
T23 1227-1239 cell_line denotes Jurkat cells
T24 1330-1355 DNA denotes 969-bp promoter fragments
T25 1409-1417 DNA denotes CRE site
T26 1452-1485 DNA denotes tissue-specific regulatory region
T27 1496-1520 DNA denotes ecto-5'-NT core promoter
T28 1564-1572 DNA denotes CRE site
T29 1584-1598 DNA denotes basal promoter

genia-medco-coref

Id Subject Object Predicate Lexical cue
C5 131-161 NP denotes the upstream regulatory region
C1 331-350 NP denotes a CAT reporter gene
C2 351-355 NP denotes that
C6 441-485 NP denotes A 768-bp upstream negative regulatory region
C3 702-705 NP denotes Sp1
C8 707-715 NP denotes Sp1/AP-2
C4 806-809 NP denotes Sp1
C7 968-998 NP denotes the upstream regulatory region
C10 1002-1008 NP denotes Jurkat
C11 1020-1030 NP denotes HeLa cells
C9 1044-1061 NP denotes the Sp1/AP-2 site
C12 1093-1108 NP denotes both cell lines
C14 1179-1191 NP denotes the CRE site
C13 1227-1239 NP denotes Jurkat cells
C15 1405-1417 NP denotes the CRE site
C17 1450-1520 NP denotes a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter
C16 1521-1525 NP denotes that
C18 1603-1606 NP denotes its
R1 C2 C1 coref-relat that,a CAT reporter gene
R2 C6 C5 coref-other A 768-bp upstream negative regulatory region,the upstream regulatory region
R3 C4 C3 coref-ident Sp1,Sp1
R4 C7 C6 coref-ident the upstream regulatory region,A 768-bp upstream negative regulatory region
R5 C9 C8 coref-ident the Sp1/AP-2 site,Sp1/AP-2
R6 C12 C10 coref-whole-part both cell lines,Jurkat
R7 C12 C11 coref-whole-part both cell lines,HeLa cells
R8 C13 C10 coref-ident Jurkat cells,Jurkat
R9 C15 C14 coref-ident the CRE site,the CRE site
R10 C16 C17 coref-relat that,a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter
R11 C18 C17 coref-pron its,a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter

pubmed-sentences-benchmark

Id Subject Object Predicate Lexical cue
S1 0-64 Sentence denotes Tissue-specific regulation of the ecto-5'-nucleotidase promoter.
S2 65-162 Sentence denotes Role of the camp response element site in mediating repression by the upstream regulatory region.
S3 163-440 Sentence denotes We have isolated the 5' region of the ecto-5'-nucleotidase (low K(m) 5'-NT) gene and established that a 969-base pair (bp) fragment confers cell-specific expression of a CAT reporter gene that correlates with the expression of endogenous ecto-5'-NT mRNA and enzymatic activity.
S4 441-635 Sentence denotes A 768-bp upstream negative regulatory region has been identified that conferred lymphocyte-specific negative regulation in a heterologous system with a 244-bp deoxycytidine kinase core promoter.
S5 636-894 Sentence denotes DNase I footprinting identified several protected areas including Sp1, Sp1/AP-2, and cAMP response element (CRE) binding sites within the 201-bp core promoter region and Sp1, NRE-2a, TCF-1/LEF-1, and Sp1/NF-AT binding sites in the upstream regulatory region.
S6 895-1109 Sentence denotes Whereas the CRE site was essential in mediating the negative activity of the upstream regulatory region in Jurkat but not in HeLa cells, mutation of the Sp1/AP-2 site decreased promoter activity in both cell lines.
S7 1110-1240 Sentence denotes Electrophoretic mobility shift assay analysis of proteins binding to the CRE site identified both ATF-1 and ATF-2 in Jurkat cells.
S8 1241-1418 Sentence denotes Finally, phorbol 12-myristate 13-acetate increased the activity of both the core and the 969-bp promoter fragments, and this increase was abrogated by mutations at the CRE site.
S9 1419-1628 Sentence denotes In summary, we have identified a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter that requires the presence of a functional CRE site within the basal promoter for its suppressive activity.

GENIAcorpus

Id Subject Object Predicate Lexical cue
T1 0-26 other_name denotes Tissue-specific regulation
T2 34-63 DNA_domain_or_region denotes ecto-5'-nucleotidase promoter
T3 77-103 DNA_domain_or_region denotes camp response element site
T4 135-161 DNA_domain_or_region denotes upstream regulatory region
T5 184-193 DNA_domain_or_region denotes 5' region
T6 201-221 protein_molecule denotes ecto-5'-nucleotidase
T7 267-294 DNA_molecule denotes 969-base pair (bp) fragment
T8 333-350 DNA_molecule denotes CAT reporter gene
T9 390-416 RNA_molecule denotes endogenous ecto-5'-NT mRNA
T10 421-439 other_name denotes enzymatic activity
T11 443-485 DNA_domain_or_region denotes 768-bp upstream negative regulatory region
T12 521-560 other_name denotes lymphocyte-specific negative regulation
T13 593-634 DNA_domain_or_region denotes 244-bp deoxycytidine kinase core promoter
T14 636-643 protein_molecule denotes DNase I
T15 867-893 DNA_domain_or_region denotes upstream regulatory region
T16 907-915 DNA_domain_or_region denotes CRE site
T17 947-964 other_name denotes negative activity
T18 972-998 DNA_domain_or_region denotes upstream regulatory region
T19 1002-1008 cell_line denotes Jurkat
T20 1020-1030 cell_line denotes HeLa cells
T21 1048-1061 DNA_domain_or_region denotes Sp1/AP-2 site
T22 1072-1089 other_name denotes promoter activity
T23 1110-1146 other_name denotes Electrophoretic mobility shift assay
T24 1183-1191 DNA_domain_or_region denotes CRE site
T25 1208-1213 protein_molecule denotes ATF-1
T26 1218-1223 protein_molecule denotes ATF-2
T27 1227-1239 cell_line denotes Jurkat cells
T28 1250-1281 other_organic_compound denotes phorbol 12-myristate 13-acetate
T29 1330-1355 DNA_molecule denotes 969-bp promoter fragments
T30 1409-1417 DNA_domain_or_region denotes CRE site
T31 1452-1485 DNA_domain_or_region denotes tissue-specific regulatory region
T32 1496-1520 DNA_domain_or_region denotes ecto-5'-NT core promoter
T33 1564-1572 DNA_domain_or_region denotes CRE site
T34 1584-1598 DNA_domain_or_region denotes basal promoter
T35 1607-1627 other_name denotes suppressive activity

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 0-6 Body_part denotes Tissue http://purl.obolibrary.org/obo/UBERON_0000479
T2 521-531 Body_part denotes lymphocyte http://purl.obolibrary.org/obo/CL_0000542
T3 1452-1458 Body_part denotes tissue http://purl.obolibrary.org/obo/UBERON_0000479

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 521-531 Cell denotes lymphocyte http://purl.obolibrary.org/obo/CL:0000542
T2 1584-1589 Cell denotes basal http://purl.obolibrary.org/obo/CL:0002324