PubMed:10428825 JSON TXT

Annnotations TAB JSON ListView MergeView

GlyCosmos600-CLO

{"project":"GlyCosmos600-CLO","denotations":[{"id":"T1","span":{"begin":103,"end":108},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":181,"end":188},"obj":"http://purl.obolibrary.org/obo/CLO_0003413"},{"id":"T3","span":{"begin":493,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0003413"},{"id":"T4","span":{"begin":570,"end":577},"obj":"http://purl.obolibrary.org/obo/CLO_0003413"},{"id":"T5","span":{"begin":743,"end":748},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.\nTwo of the most effective stimuli of gastrin release from human antral G cells are bombesin and phorbol esters. Both agonists result in activation of the protein kinase C family of isozymes, however, the exact contribution of protein kinase C to the resultant release of gastrin has been difficult to assess, possibly due to the presence of multiple protein kinase C isozymes in the G cells. The results of the present study demonstrated that the human antral G cells expressed 6 protein kinase C isozymes alpha, gamma, theta, epsilon, zeta, and mu. Of these protein kinase C, gamma and theta were translocated by stimulation of the cells by either 10 nM bombesin or 1 nM phorbol ester. Inhibition of protein kinase Cmu (localized to the Golgi complex) did not decrease bombesin-stimulated gastrin release indicating that this isozyme was not involved in the secretory process. The use of selective antagonists of the calcium-sensitive conventional protein kinase C subgroup resulted in an increase in bombesin-stimulated gastrin release and indicated that protein kinase Cgamma was involved in the desensitization of the bombesin response."}

sentences

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":109},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":110,"end":221},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":222,"end":501},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":502,"end":659},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":660,"end":796},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":797,"end":987},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":988,"end":1250},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":109},"obj":"Sentence"},{"id":"T2","span":{"begin":110,"end":221},"obj":"Sentence"},{"id":"T3","span":{"begin":222,"end":501},"obj":"Sentence"},{"id":"T4","span":{"begin":502,"end":659},"obj":"Sentence"},{"id":"T5","span":{"begin":660,"end":796},"obj":"Sentence"},{"id":"T6","span":{"begin":797,"end":987},"obj":"Sentence"},{"id":"T7","span":{"begin":988,"end":1250},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.\nTwo of the most effective stimuli of gastrin release from human antral G cells are bombesin and phorbol esters. Both agonists result in activation of the protein kinase C family of isozymes, however, the exact contribution of protein kinase C to the resultant release of gastrin has been difficult to assess, possibly due to the presence of multiple protein kinase C isozymes in the G cells. The results of the present study demonstrated that the human antral G cells expressed 6 protein kinase C isozymes alpha, gamma, theta, epsilon, zeta, and mu. Of these protein kinase C, gamma and theta were translocated by stimulation of the cells by either 10 nM bombesin or 1 nM phorbol ester. Inhibition of protein kinase Cmu (localized to the Golgi complex) did not decrease bombesin-stimulated gastrin release indicating that this isozyme was not involved in the secretory process. The use of selective antagonists of the calcium-sensitive conventional protein kinase C subgroup resulted in an increase in bombesin-stimulated gastrin release and indicated that protein kinase Cgamma was involved in the desensitization of the bombesin response."}

GlyCosmos600-FMA

pubmed-enju-pas

NCBITAXON

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":82,"end":87},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":168,"end":173},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":557,"end":562},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"}],"text":"The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.\nTwo of the most effective stimuli of gastrin release from human antral G cells are bombesin and phorbol esters. Both agonists result in activation of the protein kinase C family of isozymes, however, the exact contribution of protein kinase C to the resultant release of gastrin has been difficult to assess, possibly due to the presence of multiple protein kinase C isozymes in the G cells. The results of the present study demonstrated that the human antral G cells expressed 6 protein kinase C isozymes alpha, gamma, theta, epsilon, zeta, and mu. Of these protein kinase C, gamma and theta were translocated by stimulation of the cells by either 10 nM bombesin or 1 nM phorbol ester. Inhibition of protein kinase Cmu (localized to the Golgi complex) did not decrease bombesin-stimulated gastrin release indicating that this isozyme was not involved in the secretory process. The use of selective antagonists of the calcium-sensitive conventional protein kinase C subgroup resulted in an increase in bombesin-stimulated gastrin release and indicated that protein kinase Cgamma was involved in the desensitization of the bombesin response."}

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":848,"end":861},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005794"}],"text":"The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.\nTwo of the most effective stimuli of gastrin release from human antral G cells are bombesin and phorbol esters. Both agonists result in activation of the protein kinase C family of isozymes, however, the exact contribution of protein kinase C to the resultant release of gastrin has been difficult to assess, possibly due to the presence of multiple protein kinase C isozymes in the G cells. The results of the present study demonstrated that the human antral G cells expressed 6 protein kinase C isozymes alpha, gamma, theta, epsilon, zeta, and mu. Of these protein kinase C, gamma and theta were translocated by stimulation of the cells by either 10 nM bombesin or 1 nM phorbol ester. Inhibition of protein kinase Cmu (localized to the Golgi complex) did not decrease bombesin-stimulated gastrin release indicating that this isozyme was not involved in the secretory process. The use of selective antagonists of the calcium-sensitive conventional protein kinase C subgroup resulted in an increase in bombesin-stimulated gastrin release and indicated that protein kinase Cgamma was involved in the desensitization of the bombesin response."}

Glycosmos15-CL

{"project":"Glycosmos15-CL","denotations":[{"id":"T1","span":{"begin":181,"end":188},"obj":"Cell"},{"id":"T2","span":{"begin":493,"end":500},"obj":"Cell"},{"id":"T3","span":{"begin":570,"end":577},"obj":"Cell"},{"id":"T4","span":{"begin":637,"end":644},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000508"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000508"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000508"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0003025"}],"text":"The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.\nTwo of the most effective stimuli of gastrin release from human antral G cells are bombesin and phorbol esters. Both agonists result in activation of the protein kinase C family of isozymes, however, the exact contribution of protein kinase C to the resultant release of gastrin has been difficult to assess, possibly due to the presence of multiple protein kinase C isozymes in the G cells. The results of the present study demonstrated that the human antral G cells expressed 6 protein kinase C isozymes alpha, gamma, theta, epsilon, zeta, and mu. Of these protein kinase C, gamma and theta were translocated by stimulation of the cells by either 10 nM bombesin or 1 nM phorbol ester. Inhibition of protein kinase Cmu (localized to the Golgi complex) did not decrease bombesin-stimulated gastrin release indicating that this isozyme was not involved in the secretory process. The use of selective antagonists of the calcium-sensitive conventional protein kinase C subgroup resulted in an increase in bombesin-stimulated gastrin release and indicated that protein kinase Cgamma was involved in the desensitization of the bombesin response."}