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PubMed:10406846 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 192-208 https://glytoucan.org/Structures/Glycans/G00065MO denotes polysialic acids
T2 317-328 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T3 1396-1408 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acids

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 192-208 Glycan_Motif denotes polysialic acids https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00065MO
T2 317-328 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T3 1396-1408 Glycan_Motif denotes sialic acids https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 192-208 https://glytoucan.org/Structures/Glycans/G00065MO denotes polysialic acids
T2 317-328 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T3 1396-1408 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acids

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 1114-1117 http://purl.obolibrary.org/obo/MAT_0000213 denotes egg
T2 1131-1136 http://purl.obolibrary.org/obo/MAT_0000131 denotes sperm
T3 1131-1136 http://purl.obolibrary.org/obo/MAT_0000218 denotes sperm

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 416-431 FMAID:196776 denotes glycoconjugates
_T2 416-431 FMAID:82782 denotes glycoconjugates
_T3 751-760 FMAID:165656 denotes compounds
_T4 751-760 FMAID:67745 denotes compounds
_T5 1048-1054 FMAID:30332 denotes latter
_T6 1048-1054 FMAID:171168 denotes latter
_T7 1131-1136 FMAID:165280 denotes sperm
_T8 1247-1253 FMAID:76737 denotes column
_T9 1247-1253 FMAID:183891 denotes column
_T10 1498-1512 FMAID:165243 denotes selectivity in
_T11 1498-1512 FMAID:61795 denotes selectivity in
_T12 1636-1643 FMAID:165447 denotes protein
_T13 1636-1643 FMAID:67257 denotes protein
_T14 1852-1858 FMAID:76737 denotes column
_T15 1852-1858 FMAID:183891 denotes column
_T16 1864-1870 FMAID:30332 denotes latter
_T17 1864-1870 FMAID:171168 denotes latter

uniprot-human

Id Subject Object Predicate Lexical cue
T1 737-739 http://www.uniprot.org/uniprot/P15924 denotes DP
T2 1385-1387 http://www.uniprot.org/uniprot/P15924 denotes DP
T3 2072-2074 http://www.uniprot.org/uniprot/P15924 denotes DP
T4 1118-1136 http://www.uniprot.org/uniprot/P21754 denotes receptor for sperm
T5 1240-1242 http://www.uniprot.org/uniprot/P04746 denotes PA
T6 1240-1242 http://www.uniprot.org/uniprot/Q9UL45 denotes PA
T7 1293-1296 http://www.uniprot.org/uniprot/Q15121 denotes PED
T8 1513-1516 http://www.uniprot.org/uniprot/Q15121 denotes PED
T9 2033-2036 http://www.uniprot.org/uniprot/Q15121 denotes PED
T10 1779-1782 http://www.uniprot.org/uniprot/P28068 denotes DMB

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 737-739 http://www.uniprot.org/uniprot/E9Q557 denotes DP
T2 1385-1387 http://www.uniprot.org/uniprot/E9Q557 denotes DP
T3 2072-2074 http://www.uniprot.org/uniprot/E9Q557 denotes DP
T4 1118-1136 http://www.uniprot.org/uniprot/P10761 denotes receptor for sperm
T5 1240-1242 http://www.uniprot.org/uniprot/P00688 denotes PA

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 1118-1130 http://purl.bioontology.org/ontology/STY/T192 denotes receptor for

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-232 Sentence denotes Evaluation of high-performance anion-exchange chromatography with pulsed electrochemical and fluorometric detection for extensive application to the analysisof homologous series of oligo- and polysialic acids in bioactive molecules.
TextSentencer_T2 233-432 Sentence denotes Our previous studies have shown extensively diverse structures in oligo/polymers of sialic acid (oligo/polySia) that are expressed often in developmentally regulated manner on animal glycoconjugates.
TextSentencer_T3 433-741 Sentence denotes The aim of this study was to establish highlysensitive and specific methods that can be used to identify diverse types of oligo/polySia and thus can be applied to studies of biological phenomena associated with the differential expression of oligo/polySia chains with different degree of polymerization (DP).
TextSentencer_T4 742-1043 Sentence denotes As model compounds, we analyzed five different homologous series of oligo/polySia, (-->8Neu5Acalpha2-->)(n), (-->9Neu 5Acalpha2-->)(n), (-->8Neu5Gcalpha2-->)(n), (-->5-O(glycolyl)-Neu5Gcalpha2-->)(n), and Neu5Gc9SO(4)alpha2-->(-->5-O(glycolyl)-Neu5Gcalpha2--> )(n), ()expressed in various biopolymers.
TextSentencer_T5 1044-1137 Sentence denotes The latter two structures have recently been identified in sea urchin egg receptor for sperm.
TextSentencer_T6 1138-1447 Sentence denotes First we examined application of high-performance anion-exchange chromatography (HPAEC) on a CarboPac PA-100 column with pulsed electrochemical detection (PED) to new types of oligo/polySiacompounds and confirmed that resolution of high polymers (DP >70) of sialic acids was remarkable as reported previously.
TextSentencer_T7 1448-1644 Sentence denotes However, there are limitations in sensitivity and selectivity in PED that become significant when material is available only in a minute amount or material contained a large proportion of protein.
TextSentencer_T8 1645-1859 Sentence denotes These limitations can be circumvented by fluorometric detection of oligo/polySia tagged with 1,2-diamino-4, 5-methyl-enedioxybenzene (DMB) at the reducing terminal residues after separation on a MonoQ HR5/5 column.
TextSentencer_T9 1860-2084 Sentence denotes The latter method can be applied to any type of oligo/polySia we examined if we choose the derivatization conditions and is more sensitive and specific than the method with PED for analysis of oligo/polySia with DP up to 25.
T1 0-232 Sentence denotes Evaluation of high-performance anion-exchange chromatography with pulsed electrochemical and fluorometric detection for extensive application to the analysisof homologous series of oligo- and polysialic acids in bioactive molecules.
T2 233-432 Sentence denotes Our previous studies have shown extensively diverse structures in oligo/polymers of sialic acid (oligo/polySia) that are expressed often in developmentally regulated manner on animal glycoconjugates.
T3 433-741 Sentence denotes The aim of this study was to establish highlysensitive and specific methods that can be used to identify diverse types of oligo/polySia and thus can be applied to studies of biological phenomena associated with the differential expression of oligo/polySia chains with different degree of polymerization (DP).
T4 742-1043 Sentence denotes As model compounds, we analyzed five different homologous series of oligo/polySia, (-->8Neu5Acalpha2-->)(n), (-->9Neu 5Acalpha2-->)(n), (-->8Neu5Gcalpha2-->)(n), (-->5-O(glycolyl)-Neu5Gcalpha2-->)(n), and Neu5Gc9SO(4)alpha2-->(-->5-O(glycolyl)-Neu5Gcalpha2--> )(n), ()expressed in various biopolymers.
T5 1044-1137 Sentence denotes The latter two structures have recently been identified in sea urchin egg receptor for sperm.
T6 1138-1447 Sentence denotes First we examined application of high-performance anion-exchange chromatography (HPAEC) on a CarboPac PA-100 column with pulsed electrochemical detection (PED) to new types of oligo/polySiacompounds and confirmed that resolution of high polymers (DP >70) of sialic acids was remarkable as reported previously.
T7 1448-1644 Sentence denotes However, there are limitations in sensitivity and selectivity in PED that become significant when material is available only in a minute amount or material contained a large proportion of protein.
T8 1645-1859 Sentence denotes These limitations can be circumvented by fluorometric detection of oligo/polySia tagged with 1,2-diamino-4, 5-methyl-enedioxybenzene (DMB) at the reducing terminal residues after separation on a MonoQ HR5/5 column.
T9 1860-2084 Sentence denotes The latter method can be applied to any type of oligo/polySia we examined if we choose the derivatization conditions and is more sensitive and specific than the method with PED for analysis of oligo/polySia with DP up to 25.
T1 0-232 Sentence denotes Evaluation of high-performance anion-exchange chromatography with pulsed electrochemical and fluorometric detection for extensive application to the analysisof homologous series of oligo- and polysialic acids in bioactive molecules.
T2 233-432 Sentence denotes Our previous studies have shown extensively diverse structures in oligo/polymers of sialic acid (oligo/polySia) that are expressed often in developmentally regulated manner on animal glycoconjugates.
T3 433-741 Sentence denotes The aim of this study was to establish highlysensitive and specific methods that can be used to identify diverse types of oligo/polySia and thus can be applied to studies of biological phenomena associated with the differential expression of oligo/polySia chains with different degree of polymerization (DP).
T4 742-1043 Sentence denotes As model compounds, we analyzed five different homologous series of oligo/polySia, (-->8Neu5Acalpha2-->)(n), (-->9Neu 5Acalpha2-->)(n), (-->8Neu5Gcalpha2-->)(n), (-->5-O(glycolyl)-Neu5Gcalpha2-->)(n), and Neu5Gc9SO(4)alpha2-->(-->5-O(glycolyl)-Neu5Gcalpha2--> )(n), ()expressed in various biopolymers.
T5 1044-1137 Sentence denotes The latter two structures have recently been identified in sea urchin egg receptor for sperm.
T6 1138-1447 Sentence denotes First we examined application of high-performance anion-exchange chromatography (HPAEC) on a CarboPac PA-100 column with pulsed electrochemical detection (PED) to new types of oligo/polySiacompounds and confirmed that resolution of high polymers (DP >70) of sialic acids was remarkable as reported previously.
T7 1448-1644 Sentence denotes However, there are limitations in sensitivity and selectivity in PED that become significant when material is available only in a minute amount or material contained a large proportion of protein.
T8 1645-1859 Sentence denotes These limitations can be circumvented by fluorometric detection of oligo/polySia tagged with 1,2-diamino-4, 5-methyl-enedioxybenzene (DMB) at the reducing terminal residues after separation on a MonoQ HR5/5 column.
T9 1860-2084 Sentence denotes The latter method can be applied to any type of oligo/polySia we examined if we choose the derivatization conditions and is more sensitive and specific than the method with PED for analysis of oligo/polySia with DP up to 25.

GO-BP

Id Subject Object Predicate Lexical cue
T1 37-45 http://purl.obolibrary.org/obo/GO_0015297 denotes exchange
T2 1194-1202 http://purl.obolibrary.org/obo/GO_0015297 denotes exchange
T3 389-398 http://purl.obolibrary.org/obo/GO_0065007 denotes regulated
T4 1482-1493 http://purl.obolibrary.org/obo/GO_0046960 denotes sensitivity
T5 1989-1998 http://purl.obolibrary.org/obo/GO_0046960 denotes sensitive
T6 1755-1761 http://purl.obolibrary.org/obo/GO_0032259 denotes methyl

UBERON-AE

Id Subject Object Predicate Lexical cue
T1 120-129 http://purl.obolibrary.org/obo/UBERON_2000106 denotes extensive
T2 265-276 http://purl.obolibrary.org/obo/UBERON_2000106 denotes extensively
T3 1114-1117 http://purl.obolibrary.org/obo/UBERON_0007379 denotes egg

GlycoBiology-MAT

Id Subject Object Predicate Lexical cue
T1 1048-1054 http://purl.obolibrary.org/obo/MAT_0000488 denotes latter
T2 1114-1117 http://purl.obolibrary.org/obo/MAT_0000213 denotes egg
T3 1131-1136 http://purl.obolibrary.org/obo/MAT_0000131 denotes sperm
T4 1131-1136 http://purl.obolibrary.org/obo/MAT_0000218 denotes sperm
T5 1864-1870 http://purl.obolibrary.org/obo/MAT_0000488 denotes latter

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 120-129 http://purl.obolibrary.org/obo/UBERON_2000106 denotes extensive
PD-UBERON-AE-B_T2 265-276 http://purl.obolibrary.org/obo/UBERON_2000106 denotes extensively
PD-UBERON-AE-B_T3 1114-1117 http://purl.obolibrary.org/obo/UBERON_0007379 denotes egg

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 1114-1117 Body_part denotes egg http://purl.obolibrary.org/obo/MAT_0000213
T2 1131-1136 Body_part denotes sperm http://purl.obolibrary.org/obo/MAT_0000131|http://purl.obolibrary.org/obo/MAT_0000218

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1103-1113 OrganismTaxon denotes sea urchin 7625

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1114-1117 Body_part denotes egg http://purl.obolibrary.org/obo/UBERON_0007379
T2 1131-1136 Body_part denotes sperm http://purl.obolibrary.org/obo/CL_0000019|http://purl.obolibrary.org/obo/UBERON_0001968

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 1131-1136 Cell denotes sperm http://purl.obolibrary.org/obo/CL:0000019