PubMed:10405172 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10405172","sourcedb":"PubMed","sourceid":"10405172","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10405172","text":"Plant cell growth and differentiation may involve GAP regulation of Rac activity.\nTwo Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.","tracks":[]}