PubMed:10383770 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10383770","sourcedb":"PubMed","sourceid":"10383770","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10383770","text":"The involvement of NAD(P)H dehydrogenase subunits, NdhD3 and NdhF3, in high-affinity CO2 uptake in Synechococcus sp. PCC7002 gives evidence for multiple NDH-1 complexes with specific roles in cyanobacteria.\nRandom gene tagging was used to obtain new mutants of the marine cyanobacterium, Synechococcus sp. PCC7002, with defects in the CO2-concentrating mechanism (CCM). Two of these mutants, K22 and A41, showed poor growth at limiting CO2. Isolation and sequencing of a 6. 6 kb genomic region revealed the existence of five potential protein-coding regions, all arranged in the same transcriptional direction. These regions code for an RbcR homologue, NdhF3 (subunit 5 of type 1 NAD(P)H dehydrogenase; NDH-1 complex), NdhD3 (subunit 4 of NDH-1), ORF427 and ORF133 (hypothetical proteins). Insertional mutants in ndhD3, ndhF3 and ORF427, like A41 and K22, were all incapable of inducing high-affinity CO2 uptake and were not fully capable of inducing high-affinity HCO3- transport. ndhD3 and ndhF3 mutants displayed P700 re-reduction rates identical to wild-type cells, suggesting that NdhD3 is part of a specific NDH-1 complex that is not involved in photosynthetic cyclic electron transport. Thus, it is feasible that NdhD3, NdhF3 and ORF427 might form part of a novel NDH-1 complex located on the cytoplasmic membrane and involved in tightly coupled energization of high-affinity CO2 transport. The possibility of multiple, functionally distinct NDH-1 complexes in cyanobacteria is discussed.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":412,"end":423},"obj":"HP_0001510"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"CyanoBase","denotations":[{"id":"T1","span":{"begin":51,"end":56},"obj":"protein"},{"id":"T2","span":{"begin":61,"end":66},"obj":"protein"},{"id":"T3","span":{"begin":653,"end":658},"obj":"protein"},{"id":"T4","span":{"begin":719,"end":724},"obj":"protein"},{"id":"T5","span":{"begin":813,"end":818},"obj":"protein"},{"id":"T6","span":{"begin":820,"end":825},"obj":"protein"},{"id":"T7","span":{"begin":982,"end":987},"obj":"protein"},{"id":"T8","span":{"begin":992,"end":997},"obj":"protein"},{"id":"T9","span":{"begin":1086,"end":1091},"obj":"protein"},{"id":"T10","span":{"begin":1220,"end":1225},"obj":"protein"},{"id":"T11","span":{"begin":1227,"end":1232},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"CyanoBase"},{"subj":"T2","pred":"source","obj":"CyanoBase"},{"subj":"T3","pred":"source","obj":"CyanoBase"},{"subj":"T4","pred":"source","obj":"CyanoBase"},{"subj":"T5","pred":"source","obj":"CyanoBase"},{"subj":"T6","pred":"source","obj":"CyanoBase"},{"subj":"T7","pred":"source","obj":"CyanoBase"},{"subj":"T8","pred":"source","obj":"CyanoBase"},{"subj":"T9","pred":"source","obj":"CyanoBase"},{"subj":"T10","pred":"source","obj":"CyanoBase"},{"subj":"T11","pred":"source","obj":"CyanoBase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#e993ec","default":true},{"id":"CyanoBase","color":"#93eccf"}]}]}}