PubMed:10383450
Annnotations
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 976-987 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-100 | Sentence | denotes | Intracellular accumulation of secreted proteoglycans inhibits cationic lipid-mediated gene transfer. |
| T2 | 101-150 | Sentence | denotes | Co-transfer of glycosaminoglycans to the nucleus. |
| T3 | 151-253 | Sentence | denotes | Molecules secreted by potential target cells may interfere with cationic lipid-mediated gene transfer. |
| T4 | 254-344 | Sentence | denotes | This has been studied using human lung fibroblasts and human epidermoid lung cancer cells. |
| T5 | 345-517 | Sentence | denotes | Secreted cell medium components caused a substantial decrease both in the uptake of cationic lipid-DNA complexes (2-4-fold) and in reporter gene expression (100-1000-fold). |
| T6 | 518-667 | Sentence | denotes | Metabolic labeling of the cell medium showed that especially [35S]sulfate-labeled macromolecules competed with DNA for binding to the cationic lipid. |
| T7 | 668-793 | Sentence | denotes | Release of DNA from the cationic lipid by cell medium components was demonstrated by an ethidium bromide intercalation assay. |
| T8 | 794-892 | Sentence | denotes | In the presence of the cationic lipid, the secreted macromolecules were internalized by the cells. |
| T9 | 893-1155 | Sentence | denotes | By enzymatic digestions, it was shown that the competing macromolecules consist of chondroitin/dermatan sulfate and heparan sulfate proteoglycans and that the effects on transfection were mediated by the polyanionic glycosaminoglycan portion of the proteoglycan. |
| T10 | 1156-1295 | Sentence | denotes | Accordingly, pretreatment of cell medium with the polycationic peptide protamine sulfate abrogated the inhibitory effects on gene transfer. |
| T11 | 1296-1438 | Sentence | denotes | Fluorescence microscopy studies revealed that heparan sulfate, internalized as a complex with cationic lipids, accumulated in the cell nuclei. |
| T12 | 1439-1603 | Sentence | denotes | These results support the view that the lack of specificity of this type of gene transfer vehicle is a major hindrance to efficient and safe in vivo administration. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 976-987 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 976-987 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
| T2 | 1009-1024 | http://www.glycoepitope.jp/epitopes/EP0086 | denotes | heparan sulfate |
| T3 | 1342-1357 | http://www.glycoepitope.jp/epitopes/EP0086 | denotes | heparan sulfate |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 288-292 | http://purl.obolibrary.org/obo/MAT_0000135 | denotes | lung |
| T2 | 326-330 | http://purl.obolibrary.org/obo/MAT_0000135 | denotes | lung |
PubmedHPO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 326-337 | HP_0100526 | denotes | lung cancer |
| T2 | 331-337 | HP_0002664 | denotes | cancer |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 976-987 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 1009-1024 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T4 | 1342-1357 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 326-337 | Disease | denotes | lung cancer | http://purl.obolibrary.org/obo/MONDO_0008903 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 288-292 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/MAT_0000135 |
| T2 | 326-330 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/MAT_0000135 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 976-987 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 1009-1024 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T3 | 1342-1357 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 976-987 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 1009-1024 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T3 | 1342-1357 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
GlyCosmos15-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 326-337 | Phenotype | denotes | lung cancer | HP:0100526 |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 293-304 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 331-343 | Cell | denotes | cancer cells | http://purl.obolibrary.org/obo/CL:0001064 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 0-13 | Body_part | denotes | Intracellular | http://purl.obolibrary.org/obo/GO_0005622 |
| T2 | 142-149 | Body_part | denotes | nucleus | http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125 |
| T4 | 288-292 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/UBERON_0002048 |
| T5 | 293-304 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T6 | 326-330 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/UBERON_0002048 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 282-287 | Organism | denotes | human | 9606 |
| T2 | 309-314 | Organism | denotes | human | 9606 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-100 | Sentence | denotes | Intracellular accumulation of secreted proteoglycans inhibits cationic lipid-mediated gene transfer. |
| T2 | 101-150 | Sentence | denotes | Co-transfer of glycosaminoglycans to the nucleus. |
| T3 | 151-253 | Sentence | denotes | Molecules secreted by potential target cells may interfere with cationic lipid-mediated gene transfer. |
| T4 | 254-344 | Sentence | denotes | This has been studied using human lung fibroblasts and human epidermoid lung cancer cells. |
| T5 | 345-517 | Sentence | denotes | Secreted cell medium components caused a substantial decrease both in the uptake of cationic lipid-DNA complexes (2-4-fold) and in reporter gene expression (100-1000-fold). |
| T6 | 518-667 | Sentence | denotes | Metabolic labeling of the cell medium showed that especially [35S]sulfate-labeled macromolecules competed with DNA for binding to the cationic lipid. |
| T7 | 668-793 | Sentence | denotes | Release of DNA from the cationic lipid by cell medium components was demonstrated by an ethidium bromide intercalation assay. |
| T8 | 794-892 | Sentence | denotes | In the presence of the cationic lipid, the secreted macromolecules were internalized by the cells. |
| T9 | 893-1155 | Sentence | denotes | By enzymatic digestions, it was shown that the competing macromolecules consist of chondroitin/dermatan sulfate and heparan sulfate proteoglycans and that the effects on transfection were mediated by the polyanionic glycosaminoglycan portion of the proteoglycan. |
| T10 | 1156-1295 | Sentence | denotes | Accordingly, pretreatment of cell medium with the polycationic peptide protamine sulfate abrogated the inhibitory effects on gene transfer. |
| T11 | 1296-1438 | Sentence | denotes | Fluorescence microscopy studies revealed that heparan sulfate, internalized as a complex with cationic lipids, accumulated in the cell nuclei. |
| T12 | 1439-1603 | Sentence | denotes | These results support the view that the lack of specificity of this type of gene transfer vehicle is a major hindrance to efficient and safe in vivo administration. |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 976-987 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 1009-1024 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T3 | 1342-1357 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
GlyCosmos15-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 326-337 | Disease | denotes | lung cancer | MONDO:0005138|MONDO:0008903 |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 288-292 | Body_part | denotes | lung | FMA:7195 |
| T2 | 293-304 | Body_part | denotes | fibroblasts | FMA:63877 |
| T3 | 326-330 | Body_part | denotes | lung | FMA:7195 |
GlyCosmos15-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 288-292 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/MAT_0000135 |
| T2 | 326-330 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/MAT_0000135 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 282-287 | OrganismTaxon | denotes | human | 9606 |
| T2 | 309-314 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 0-13 | Body_part | denotes | Intracellular | http://purl.obolibrary.org/obo/GO_0005622 |
| T2 | 142-149 | Body_part | denotes | nucleus | http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125 |
| T4 | 288-292 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/UBERON_0002048 |
| T5 | 293-304 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T6 | 326-330 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/UBERON_0002048 |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 183-195 | Phenotype | denotes | target cells | HP:0034280 |
| T2 | 326-337 | Phenotype | denotes | lung cancer | HP:0100526 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 293-304 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 331-343 | Cell | denotes | cancer cells | http://purl.obolibrary.org/obo/CL:0001064 |