PubMed:10380113 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10380113","sourcedb":"PubMed","sourceid":"10380113","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10380113","text":"Cloning and sequence of a cDNA for a highly basic protease from the digestive juice of the silkworm, Bombyx mori.\nA serine protease of the silkworm, Bombyx mori, with an isoelectric point of pH 10-11 and a pH optimum for succinyl-Leu-Leu-Val-Tyr-MCA degrading activity of about 10, was found in a 0.33 M NaCl-eluted fraction obtained from cation-exchange chromatography of digestive juice. The activity of the enzyme was strongly inhibited by chymostatin and PMSF, indicating that the protease is a chymotrypsin-like serine protease. The N-terminal amino acid sequence of the protease was determined, and a full-length cDNA clone (0.92 kbp) which was isolated from a midgut cDNA library was sequenced. The cDNA encodes a pre-proenzyme of 284 amino acids with a pro-segment of 50 amino acids and mature protein of 234 amino acids. From its primary structure, the predicted molecular mass of the mature protein is 24.5 kDa. A sequence comparison of the Bombyx highly basic protease with other serine proteases revealed that this enzyme is a mammalian-type serine protease with a catalytic triad consisting of His45, Asp92 and Ser186. A large number of Arg residues are encoded by the cDNA which may be responsible for its stability and/or function in the alkaline condition, by remaining charged at high pH.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":91,"end":99},"obj":"Species:7091"},{"id":"T2","span":{"begin":101,"end":112},"obj":"Species:7091"},{"id":"T3","span":{"begin":139,"end":147},"obj":"Species:7091"},{"id":"T4","span":{"begin":149,"end":160},"obj":"Species:7091"},{"id":"T5","span":{"begin":221,"end":237},"obj":"Chemical:-"},{"id":"T6","span":{"begin":238,"end":241},"obj":"Chemical:MESH:D014633"},{"id":"T7","span":{"begin":242,"end":245},"obj":"Chemical:MESH:D014443"},{"id":"T8","span":{"begin":304,"end":308},"obj":"Chemical:MESH:D012965"},{"id":"T9","span":{"begin":459,"end":463},"obj":"Chemical:MESH:D010664"},{"id":"T10","span":{"begin":1039,"end":1048},"obj":"Species:9606"},{"id":"T11","span":{"begin":1107,"end":1112},"obj":"Chemical:-"},{"id":"T12","span":{"begin":1114,"end":1119},"obj":"Chemical:-"},{"id":"T13","span":{"begin":1124,"end":1130},"obj":"Chemical:-"},{"id":"T14","span":{"begin":1150,"end":1153},"obj":"Chemical:MESH:D001120"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"},{"subj":"T14","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":91,"end":99},"obj":"Species:7091"},{"id":"T10","span":{"begin":1039,"end":1048},"obj":"Species:9606"},{"id":"T11","span":{"begin":1107,"end":1112},"obj":"Chemical:-"},{"id":"T12","span":{"begin":1114,"end":1119},"obj":"Chemical:-"},{"id":"T13","span":{"begin":1124,"end":1130},"obj":"Chemical:-"},{"id":"T14","span":{"begin":1150,"end":1153},"obj":"Chemical:MESH:D001120"},{"id":"T2","span":{"begin":101,"end":112},"obj":"Species:7091"},{"id":"T3","span":{"begin":139,"end":147},"obj":"Species:7091"},{"id":"T4","span":{"begin":149,"end":160},"obj":"Species:7091"},{"id":"T5","span":{"begin":221,"end":237},"obj":"Chemical:-"},{"id":"T6","span":{"begin":238,"end":241},"obj":"Chemical:MESH:D014633"},{"id":"T7","span":{"begin":242,"end":245},"obj":"Chemical:MESH:D014443"},{"id":"T8","span":{"begin":304,"end":308},"obj":"Chemical:MESH:D012965"},{"id":"T9","span":{"begin":459,"end":463},"obj":"Chemical:MESH:D010664"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#93eca2","default":true},{"id":"silkwormbase","color":"#ec939e"}]}]}}