> top > docs > PubMed:10362839 > annotations

PubMed:10362839 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-143 Sentence denotes Identification and characterization of a novel UDP-GalNAc:GlcAbeta-R alpha1,4-N-acetylgalactosaminyltransferase from a human sarcoma cell line.
TextSentencer_T2 144-257 Sentence denotes We recently discovered a novel alpha-N-acetylgalactosaminyltransferase in fetal bovine serum (Kitagawa et al., J.
TextSentencer_T3 258-263 Sentence denotes Biol.
TextSentencer_T4 264-556 Sentence denotes Chem., 270, 22190-22195, 1995) and also in mouse mast cytoma cells (Lidholt et al., Glycoconjugate J., 14, 737-742, 1997), which catalyzed the transfer of an alpha-GalNAc residue to the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, derived from proteoglycans.
TextSentencer_T5 557-762 Sentence denotes In this study, we characterized this enzyme using a preparation obtained from the serum-free culture medium of a human sarcoma (malignant fibrous histiocytoma) cell line by phenyl-Sepharose chromatography.
TextSentencer_T6 763-1043 Sentence denotes Structural characterization by1H NMR spectroscopy of the reaction product using the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, as a substrate demonstrated that the enzyme was a UDP-GalNAc:GlcAbeta1-R alpha1,4-N -acetylgalactosaminyltransferase.
TextSentencer_T7 1044-1126 Sentence denotes This is the first identification of an alpha1,4-N-acetylgalactosaminyltransferase.
TextSentencer_T8 1127-1478 Sentence denotes Using N -acetylchondrosine GlcAbeta1-3GalNAc as an alternative substrate, the enzyme required divalent cations for the transferase reaction, with maximal activity at 20 mM Mn2+and exhibited a dual optimum at pH 6.5 and pH 7.4 depending upon the buffers used, with the highest activity in a 50 mM 2-( N -morpholino)ethanesulfonic acid buffer at pH 6.5.
TextSentencer_T9 1479-1646 Sentence denotes The apparent Km values obtained for N -acetylchondrosine, the linkage tetrasaccharide-serine, and UDP-GalNAc were 1060 microM, 188 microM, and 27 microM, respectively.
TextSentencer_T10 1647-1747 Sentence denotes This suggested that the linkage tetrasaccharide-serine was a good acceptor substrate for the enzyme.
TextSentencer_T11 1748-2021 Sentence denotes In addition, the enzyme utilized glucuronylneolactotetraosylceramide GlcAbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4G lcbeta1-1Cer but not sulfoglucuronylneolactotetraosylceramide GlcA(3-O -sulfate)beta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Gl cbeta1-1Cer as acceptor substrates.
TextSentencer_T12 2022-2171 Sentence denotes The possibility of involvement of this enzyme in the biosynthesis of glycosaminoglycan as well as other GlcA-containing glycoconjugates is discussed.
T1 0-143 Sentence denotes Identification and characterization of a novel UDP-GalNAc:GlcAbeta-R alpha1,4-N-acetylgalactosaminyltransferase from a human sarcoma cell line.
T2 144-556 Sentence denotes We recently discovered a novel alpha-N-acetylgalactosaminyltransferase in fetal bovine serum (Kitagawa et al., J. Biol. Chem., 270, 22190-22195, 1995) and also in mouse mast cytoma cells (Lidholt et al., Glycoconjugate J., 14, 737-742, 1997), which catalyzed the transfer of an alpha-GalNAc residue to the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, derived from proteoglycans.
T3 557-762 Sentence denotes In this study, we characterized this enzyme using a preparation obtained from the serum-free culture medium of a human sarcoma (malignant fibrous histiocytoma) cell line by phenyl-Sepharose chromatography.
T4 763-1043 Sentence denotes Structural characterization by1H NMR spectroscopy of the reaction product using the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, as a substrate demonstrated that the enzyme was a UDP-GalNAc:GlcAbeta1-R alpha1,4-N -acetylgalactosaminyltransferase.
T5 1044-1126 Sentence denotes This is the first identification of an alpha1,4-N-acetylgalactosaminyltransferase.
T6 1127-1478 Sentence denotes Using N -acetylchondrosine GlcAbeta1-3GalNAc as an alternative substrate, the enzyme required divalent cations for the transferase reaction, with maximal activity at 20 mM Mn2+and exhibited a dual optimum at pH 6.5 and pH 7.4 depending upon the buffers used, with the highest activity in a 50 mM 2-( N -morpholino)ethanesulfonic acid buffer at pH 6.5.
T7 1479-1646 Sentence denotes The apparent Km values obtained for N -acetylchondrosine, the linkage tetrasaccharide-serine, and UDP-GalNAc were 1060 microM, 188 microM, and 27 microM, respectively.
T8 1647-1747 Sentence denotes This suggested that the linkage tetrasaccharide-serine was a good acceptor substrate for the enzyme.
T9 1748-2021 Sentence denotes In addition, the enzyme utilized glucuronylneolactotetraosylceramide GlcAbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4G lcbeta1-1Cer but not sulfoglucuronylneolactotetraosylceramide GlcA(3-O -sulfate)beta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Gl cbeta1-1Cer as acceptor substrates.
T10 2022-2171 Sentence denotes The possibility of involvement of this enzyme in the biosynthesis of glycosaminoglycan as well as other GlcA-containing glycoconjugates is discussed.
T1 0-143 Sentence denotes Identification and characterization of a novel UDP-GalNAc:GlcAbeta-R alpha1,4-N-acetylgalactosaminyltransferase from a human sarcoma cell line.
T2 144-257 Sentence denotes We recently discovered a novel alpha-N-acetylgalactosaminyltransferase in fetal bovine serum (Kitagawa et al., J.
T3 258-263 Sentence denotes Biol.
T4 264-556 Sentence denotes Chem., 270, 22190-22195, 1995) and also in mouse mast cytoma cells (Lidholt et al., Glycoconjugate J., 14, 737-742, 1997), which catalyzed the transfer of an alpha-GalNAc residue to the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, derived from proteoglycans.
T5 557-762 Sentence denotes In this study, we characterized this enzyme using a preparation obtained from the serum-free culture medium of a human sarcoma (malignant fibrous histiocytoma) cell line by phenyl-Sepharose chromatography.
T6 763-1043 Sentence denotes Structural characterization by1H NMR spectroscopy of the reaction product using the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, as a substrate demonstrated that the enzyme was a UDP-GalNAc:GlcAbeta1-R alpha1,4-N -acetylgalactosaminyltransferase.
T7 1044-1126 Sentence denotes This is the first identification of an alpha1,4-N-acetylgalactosaminyltransferase.
T8 1127-1478 Sentence denotes Using N -acetylchondrosine GlcAbeta1-3GalNAc as an alternative substrate, the enzyme required divalent cations for the transferase reaction, with maximal activity at 20 mM Mn2+and exhibited a dual optimum at pH 6.5 and pH 7.4 depending upon the buffers used, with the highest activity in a 50 mM 2-( N -morpholino)ethanesulfonic acid buffer at pH 6.5.
T9 1479-1646 Sentence denotes The apparent Km values obtained for N -acetylchondrosine, the linkage tetrasaccharide-serine, and UDP-GalNAc were 1060 microM, 188 microM, and 27 microM, respectively.
T10 1647-1747 Sentence denotes This suggested that the linkage tetrasaccharide-serine was a good acceptor substrate for the enzyme.
T11 1748-2021 Sentence denotes In addition, the enzyme utilized glucuronylneolactotetraosylceramide GlcAbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4G lcbeta1-1Cer but not sulfoglucuronylneolactotetraosylceramide GlcA(3-O -sulfate)beta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Gl cbeta1-1Cer as acceptor substrates.
T12 2022-2171 Sentence denotes The possibility of involvement of this enzyme in the biosynthesis of glycosaminoglycan as well as other GlcA-containing glycoconjugates is discussed.

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 231-236 FMAID:167330 denotes serum
_T2 313-330 FMAID:66784 denotes mast cytoma cells
_T3 313-330 FMAID:164938 denotes mast cytoma cells
_T4 325-330 FMAID:68646 denotes cells
_T5 325-330 FMAID:169002 denotes cells
_T6 348-362 FMAID:82782 denotes Glycoconjugate
_T7 348-362 FMAID:196776 denotes Glycoconjugate
_T8 474-480 FMAID:196753 denotes serine
_T9 474-480 FMAID:165609 denotes serine
_T10 474-480 FMAID:67710 denotes serine
_T11 474-480 FMAID:82764 denotes serine
_T12 542-555 FMAID:63015 denotes proteoglycans
_T13 542-555 FMAID:167397 denotes proteoglycans
_T14 639-644 FMAID:167330 denotes serum
_T15 871-877 FMAID:67710 denotes serine
_T16 871-877 FMAID:165609 denotes serine
_T17 871-877 FMAID:196753 denotes serine
_T18 871-877 FMAID:82764 denotes serine
_T19 1565-1571 FMAID:67710 denotes serine
_T20 1565-1571 FMAID:82764 denotes serine
_T21 1565-1571 FMAID:196753 denotes serine
_T22 1565-1571 FMAID:165609 denotes serine
_T23 1598-1604 FMAID:165145 denotes microM
_T24 1610-1616 FMAID:165145 denotes microM
_T25 1625-1631 FMAID:165145 denotes microM
_T26 1695-1701 FMAID:82764 denotes serine
_T27 1695-1701 FMAID:67710 denotes serine
_T28 1695-1701 FMAID:165609 denotes serine
_T29 1695-1701 FMAID:196753 denotes serine
_T30 2091-2108 FMAID:167395 denotes glycosaminoglycan
_T31 2091-2108 FMAID:63011 denotes glycosaminoglycan
_T32 2142-2157 FMAID:82782 denotes glycoconjugates
_T33 2142-2157 FMAID:196776 denotes glycoconjugates

uniprot-human

Id Subject Object Predicate Lexical cue
T1 1246-1257 http://www.uniprot.org/uniprot/Q99484 denotes transferase

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 1246-1257 http://www.uniprot.org/uniprot/P38649 denotes transferase

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 125-132 http://purl.bioontology.org/ontology/NCBITAXON/47206 denotes sarcoma
T2 325-330 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T3 676-683 http://purl.bioontology.org/ontology/NCBITAXON/47206 denotes sarcoma
T4 1221-1229 http://purl.bioontology.org/ontology/NCBITAXON/433098 denotes divalent

GO-BP

Id Subject Object Predicate Lexical cue
T1 247-249 http://purl.obolibrary.org/obo/GO_0004306 denotes et
T2 340-342 http://purl.obolibrary.org/obo/GO_0004306 denotes et
T3 1925-1929 http://purl.obolibrary.org/obo/GO_0043879 denotes GlcA
T4 2126-2130 http://purl.obolibrary.org/obo/GO_0043879 denotes GlcA
T5 1935-1942 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T6 2075-2087 http://purl.obolibrary.org/obo/GO_0009058 denotes biosynthesis
T7 2075-2108 http://purl.obolibrary.org/obo/GO_0006024 denotes biosynthesis of glycosaminoglycan

GO-CC

Id Subject Object Predicate Lexical cue
T1 133-137 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2 325-330 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3 524-527 http://purl.obolibrary.org/obo/GO_0005790 denotes Ser
T4 921-924 http://purl.obolibrary.org/obo/GO_0005790 denotes Ser

UBERON-AE

Id Subject Object Predicate Lexical cue
T1 231-236 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T2 639-644 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 119-124 http://edamontology.org/topic_2815 denotes human
T2 450-457 http://edamontology.org/topic_0102 denotes linkage
T3 565-570 http://edamontology.org/topic_3678 denotes study
T4 670-675 http://edamontology.org/topic_2815 denotes human
T5 796-799 http://edamontology.org/topic_0593 denotes NMR
T6 796-812 http://edamontology.org/topic_0593 denotes NMR spectroscopy
T7 800-812 http://edamontology.org/topic_0593 denotes spectroscopy
T8 847-854 http://edamontology.org/topic_0102 denotes linkage
T9 1541-1548 http://edamontology.org/topic_0102 denotes linkage
T10 1671-1678 http://edamontology.org/topic_0102 denotes linkage

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 435-442 http://edamontology.org/data_1756 denotes residue
T2 763-773 http://edamontology.org/data_0883 denotes Structural
T3 941-953 http://edamontology.org/operation_2246 denotes demonstrated
T4 1335-1337 http://edamontology.org/format_1997 denotes pH
T5 1346-1348 http://edamontology.org/format_1997 denotes pH
T6 1471-1473 http://edamontology.org/format_1997 denotes pH
T7 1492-1494 http://edamontology.org/data_0910 denotes Km

Lectin

Id Subject Object Predicate Lexical cue
Lectin_T1 250-252 https://acgg.asia/db/lfdb/LfDB0344 denotes al
Lectin_T2 343-345 https://acgg.asia/db/lfdb/LfDB0344 denotes al

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 428-434 "http://rdf.glycoinfo.org/glycan/G23425WZ" denotes GalNAc
GlycanIUPAC_T2 1581-1587 "http://rdf.glycoinfo.org/glycan/G23425WZ" denotes GalNAc
GlycanIUPAC_T3 428-434 "http://rdf.glycoinfo.org/glycan/G02874VH" denotes GalNAc
GlycanIUPAC_T4 1581-1587 "http://rdf.glycoinfo.org/glycan/G02874VH" denotes GalNAc
GlycanIUPAC_T5 428-434 "http://rdf.glycoinfo.org/glycan/G99699DW" denotes GalNAc
GlycanIUPAC_T6 1581-1587 "http://rdf.glycoinfo.org/glycan/G99699DW" denotes GalNAc
GlycanIUPAC_T7 428-434 "http://rdf.glycoinfo.org/glycan/G22074RM" denotes GalNAc
GlycanIUPAC_T8 1581-1587 "http://rdf.glycoinfo.org/glycan/G22074RM" denotes GalNAc
GlycanIUPAC_T9 428-434 "http://rdf.glycoinfo.org/glycan/G48535VZ" denotes GalNAc
GlycanIUPAC_T10 1581-1587 "http://rdf.glycoinfo.org/glycan/G48535VZ" denotes GalNAc
GlycanIUPAC_T11 428-434 "http://rdf.glycoinfo.org/glycan/G39738WL" denotes GalNAc
GlycanIUPAC_T12 1581-1587 "http://rdf.glycoinfo.org/glycan/G39738WL" denotes GalNAc
GlycanIUPAC_T13 428-434 "http://rdf.glycoinfo.org/glycan/G42313WU" denotes GalNAc
GlycanIUPAC_T14 1581-1587 "http://rdf.glycoinfo.org/glycan/G42313WU" denotes GalNAc
GlycanIUPAC_T15 428-434 "http://rdf.glycoinfo.org/glycan/G00393CK" denotes GalNAc
GlycanIUPAC_T16 1581-1587 "http://rdf.glycoinfo.org/glycan/G00393CK" denotes GalNAc
GlycanIUPAC_T17 428-434 "http://rdf.glycoinfo.org/glycan/G42649EX" denotes GalNAc
GlycanIUPAC_T18 1581-1587 "http://rdf.glycoinfo.org/glycan/G42649EX" denotes GalNAc
GlycanIUPAC_T19 428-434 "http://rdf.glycoinfo.org/glycan/G46880SB" denotes GalNAc
GlycanIUPAC_T20 1581-1587 "http://rdf.glycoinfo.org/glycan/G46880SB" denotes GalNAc
GlycanIUPAC_T21 428-434 "http://rdf.glycoinfo.org/glycan/G75599IR" denotes GalNAc
GlycanIUPAC_T22 1581-1587 "http://rdf.glycoinfo.org/glycan/G75599IR" denotes GalNAc
GlycanIUPAC_T23 428-434 "http://rdf.glycoinfo.org/glycan/G58985MU" denotes GalNAc
GlycanIUPAC_T24 1581-1587 "http://rdf.glycoinfo.org/glycan/G58985MU" denotes GalNAc
GlycanIUPAC_T25 428-434 "http://rdf.glycoinfo.org/glycan/G92517PO" denotes GalNAc
GlycanIUPAC_T26 1581-1587 "http://rdf.glycoinfo.org/glycan/G92517PO" denotes GalNAc
GlycanIUPAC_T27 428-434 "http://rdf.glycoinfo.org/glycan/G88512YL" denotes GalNAc
GlycanIUPAC_T28 1581-1587 "http://rdf.glycoinfo.org/glycan/G88512YL" denotes GalNAc
GlycanIUPAC_T29 428-434 "http://rdf.glycoinfo.org/glycan/G41473NX" denotes GalNAc
GlycanIUPAC_T30 1581-1587 "http://rdf.glycoinfo.org/glycan/G41473NX" denotes GalNAc
GlycanIUPAC_T31 428-434 "http://rdf.glycoinfo.org/glycan/G71089RB" denotes GalNAc
GlycanIUPAC_T32 1581-1587 "http://rdf.glycoinfo.org/glycan/G71089RB" denotes GalNAc
GlycanIUPAC_T33 428-434 "http://rdf.glycoinfo.org/glycan/G73485GZ" denotes GalNAc
GlycanIUPAC_T34 1581-1587 "http://rdf.glycoinfo.org/glycan/G73485GZ" denotes GalNAc
GlycanIUPAC_T35 428-434 "http://rdf.glycoinfo.org/glycan/G61406KC" denotes GalNAc
GlycanIUPAC_T36 1581-1587 "http://rdf.glycoinfo.org/glycan/G61406KC" denotes GalNAc
GlycanIUPAC_T37 428-434 "http://rdf.glycoinfo.org/glycan/G34412GZ" denotes GalNAc
GlycanIUPAC_T38 1581-1587 "http://rdf.glycoinfo.org/glycan/G34412GZ" denotes GalNAc
GlycanIUPAC_T39 428-434 "http://rdf.glycoinfo.org/glycan/G67209FP" denotes GalNAc
GlycanIUPAC_T40 1581-1587 "http://rdf.glycoinfo.org/glycan/G67209FP" denotes GalNAc
GlycanIUPAC_T41 428-434 "http://rdf.glycoinfo.org/glycan/G61442IL" denotes GalNAc
GlycanIUPAC_T42 1581-1587 "http://rdf.glycoinfo.org/glycan/G61442IL" denotes GalNAc
GlycanIUPAC_T43 428-434 "http://rdf.glycoinfo.org/glycan/G93729MV" denotes GalNAc
GlycanIUPAC_T44 1581-1587 "http://rdf.glycoinfo.org/glycan/G93729MV" denotes GalNAc
GlycanIUPAC_T45 428-434 "http://rdf.glycoinfo.org/glycan/G92144AE" denotes GalNAc
GlycanIUPAC_T46 1581-1587 "http://rdf.glycoinfo.org/glycan/G92144AE" denotes GalNAc
GlycanIUPAC_T47 1925-1929 "http://rdf.glycoinfo.org/glycan/G91489RT" denotes GlcA
GlycanIUPAC_T48 2126-2130 "http://rdf.glycoinfo.org/glycan/G91489RT" denotes GlcA
GlycanIUPAC_T49 1925-1929 "http://rdf.glycoinfo.org/glycan/G54004DL" denotes GlcA
GlycanIUPAC_T50 2126-2130 "http://rdf.glycoinfo.org/glycan/G54004DL" denotes GlcA
GlycanIUPAC_T51 1925-1929 "http://rdf.glycoinfo.org/glycan/G90208ZS" denotes GlcA
GlycanIUPAC_T52 2126-2130 "http://rdf.glycoinfo.org/glycan/G90208ZS" denotes GlcA
GlycanIUPAC_T53 1925-1929 "http://rdf.glycoinfo.org/glycan/G47973RQ" denotes GlcA
GlycanIUPAC_T54 2126-2130 "http://rdf.glycoinfo.org/glycan/G47973RQ" denotes GlcA
GlycanIUPAC_T55 1925-1929 "http://rdf.glycoinfo.org/glycan/G35471RR" denotes GlcA
GlycanIUPAC_T56 2126-2130 "http://rdf.glycoinfo.org/glycan/G35471RR" denotes GlcA
GlycanIUPAC_T57 1925-1929 "http://rdf.glycoinfo.org/glycan/G45399NV" denotes GlcA
GlycanIUPAC_T58 2126-2130 "http://rdf.glycoinfo.org/glycan/G45399NV" denotes GlcA
GlycanIUPAC_T59 1925-1929 "http://rdf.glycoinfo.org/glycan/G52061FU" denotes GlcA
GlycanIUPAC_T60 2126-2130 "http://rdf.glycoinfo.org/glycan/G52061FU" denotes GlcA
GlycanIUPAC_T61 1925-1929 "http://rdf.glycoinfo.org/glycan/G10736YH" denotes GlcA
GlycanIUPAC_T62 2126-2130 "http://rdf.glycoinfo.org/glycan/G10736YH" denotes GlcA
GlycanIUPAC_T63 1925-1929 "http://rdf.glycoinfo.org/glycan/G30254CI" denotes GlcA
GlycanIUPAC_T64 2126-2130 "http://rdf.glycoinfo.org/glycan/G30254CI" denotes GlcA
GlycanIUPAC_T65 1925-1929 "http://rdf.glycoinfo.org/glycan/G57856MN" denotes GlcA
GlycanIUPAC_T66 2126-2130 "http://rdf.glycoinfo.org/glycan/G57856MN" denotes GlcA
GlycanIUPAC_T67 1925-1929 "http://rdf.glycoinfo.org/glycan/G95754KF" denotes GlcA
GlycanIUPAC_T68 2126-2130 "http://rdf.glycoinfo.org/glycan/G95754KF" denotes GlcA
GlycanIUPAC_T69 1925-1929 "http://rdf.glycoinfo.org/glycan/G90067UQ" denotes GlcA
GlycanIUPAC_T70 2126-2130 "http://rdf.glycoinfo.org/glycan/G90067UQ" denotes GlcA

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 231-236 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
PD-UBERON-AE-B_T2 639-644 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 676-683 HP_0100242 denotes sarcoma
T2 703-715 HP_0012315 denotes histiocytoma

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 125-132 Phenotype denotes sarcoma HP:0100242
T2 676-683 Phenotype denotes sarcoma HP:0100242
T3 703-715 Phenotype denotes histiocytoma HP:0012315

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 125-132 Disease denotes sarcoma http://purl.obolibrary.org/obo/MONDO_0005089
T2 676-683 Disease denotes sarcoma http://purl.obolibrary.org/obo/MONDO_0005089
T3 685-715 Disease denotes malignant fibrous histiocytoma http://purl.obolibrary.org/obo/MONDO_0002142

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T3 703-715 Phenotype denotes histiocytoma HP:0012315
T1 125-132 Phenotype denotes sarcoma HP:0100242
T2 676-683 Phenotype denotes sarcoma HP:0100242

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 428-434 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2 1581-1587 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T3 51-57 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T5 980-986 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 428-434 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2 1581-1587 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 119-124 Organism denotes human 9606
T2 224-230 Organism denotes bovine 9913
T3 307-312 Organism denotes mouse 10088|10090
T5 670-675 Organism denotes human 9606

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 125-132 Disease denotes sarcoma MONDO:0005089
T2 676-683 Disease denotes sarcoma MONDO:0005089
T3 685-715 Disease denotes malignant fibrous histiocytoma MONDO:0002142

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-143 Sentence denotes Identification and characterization of a novel UDP-GalNAc:GlcAbeta-R alpha1,4-N-acetylgalactosaminyltransferase from a human sarcoma cell line.
T2 144-556 Sentence denotes We recently discovered a novel alpha-N-acetylgalactosaminyltransferase in fetal bovine serum (Kitagawa et al., J. Biol. Chem., 270, 22190-22195, 1995) and also in mouse mast cytoma cells (Lidholt et al., Glycoconjugate J., 14, 737-742, 1997), which catalyzed the transfer of an alpha-GalNAc residue to the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, derived from proteoglycans.
T3 557-762 Sentence denotes In this study, we characterized this enzyme using a preparation obtained from the serum-free culture medium of a human sarcoma (malignant fibrous histiocytoma) cell line by phenyl-Sepharose chromatography.
T4 763-1043 Sentence denotes Structural characterization by1H NMR spectroscopy of the reaction product using the linkage tetrasaccharide-serine, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, as a substrate demonstrated that the enzyme was a UDP-GalNAc:GlcAbeta1-R alpha1,4-N -acetylgalactosaminyltransferase.
T5 1044-1126 Sentence denotes This is the first identification of an alpha1,4-N-acetylgalactosaminyltransferase.
T6 1127-1478 Sentence denotes Using N -acetylchondrosine GlcAbeta1-3GalNAc as an alternative substrate, the enzyme required divalent cations for the transferase reaction, with maximal activity at 20 mM Mn2+and exhibited a dual optimum at pH 6.5 and pH 7.4 depending upon the buffers used, with the highest activity in a 50 mM 2-( N -morpholino)ethanesulfonic acid buffer at pH 6.5.
T7 1479-1646 Sentence denotes The apparent Km values obtained for N -acetylchondrosine, the linkage tetrasaccharide-serine, and UDP-GalNAc were 1060 microM, 188 microM, and 27 microM, respectively.
T8 1647-1747 Sentence denotes This suggested that the linkage tetrasaccharide-serine was a good acceptor substrate for the enzyme.
T9 1748-2021 Sentence denotes In addition, the enzyme utilized glucuronylneolactotetraosylceramide GlcAbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4G lcbeta1-1Cer but not sulfoglucuronylneolactotetraosylceramide GlcA(3-O -sulfate)beta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Gl cbeta1-1Cer as acceptor substrates.
T10 2022-2171 Sentence denotes The possibility of involvement of this enzyme in the biosynthesis of glycosaminoglycan as well as other GlcA-containing glycoconjugates is discussed.

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 231-236 Body_part denotes serum FMA:63083
T2 639-644 Body_part denotes serum FMA:63083

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 119-124 OrganismTaxon denotes human 9606
T2 224-230 OrganismTaxon denotes bovine 9913
T3 307-312 OrganismTaxon denotes mouse 10088|10090
T5 670-675 OrganismTaxon denotes human 9606