PubMed:10359895 JSON TXT

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jnlpba-st-training

semrep-sample

pubmed-sentences-benchmark

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"S2","span":{"begin":99,"end":228},"obj":"Sentence"},{"id":"S3","span":{"begin":229,"end":334},"obj":"Sentence"},{"id":"S4","span":{"begin":335,"end":442},"obj":"Sentence"},{"id":"S5","span":{"begin":443,"end":671},"obj":"Sentence"},{"id":"S6","span":{"begin":672,"end":894},"obj":"Sentence"},{"id":"S7","span":{"begin":895,"end":993},"obj":"Sentence"},{"id":"S8","span":{"begin":994,"end":1088},"obj":"Sentence"},{"id":"S9","span":{"begin":1089,"end":1236},"obj":"Sentence"},{"id":"S10","span":{"begin":1237,"end":1423},"obj":"Sentence"},{"id":"S11","span":{"begin":1424,"end":1580},"obj":"Sentence"},{"id":"S12","span":{"begin":1581,"end":1772},"obj":"Sentence"}],"text":"Binding of YY1 and Oct1 to a novel element that downregulates expression of IL-5 in human T cells.\nBACKGROUND: IL-5 controls development of eosinophilia and has been shown to be involved in the pathogenesis of allergic diseases. In both atopic and nonatopic asthma, elevated IL-5 has been detected in peripheral blood and the airways. IL-5 is produced mainly by activated T cells, and its expression is regulated at the transcriptional level.\nOBJECTIVE: This study focuses on the functional analysis of the human IL-5 (hIL-5) promoter and characterization of cis -regulatory elements and transcription factors involved in the suppression of IL-5 transcription in T cells.\nMETHODS: Methods used in this study include DNase I footprint assays, electrophoretic mobility shift assays, and functional analysis by mammalian cell transfection involving deletion analysis and site-directed mutagenesis.\nRESULTS: We identified 5 protein binding regions (BRs) located within the proximal hIL-5 promoter. Functional analysis indicates that the BRs are involved in control of hIL-5 promoter activity. Two of these regions, BR3 and BR4 located at positions -102 to -73, have not previously been described as regulators of IL-5 expression in T cells. We show that the BR3 sequence contains a novel negative regulatory element located at positions -90 to -79 of the hIL-5 promoter, which binds Oct1, octamer-like, and YY1 nuclear factors. Substitution mutations, which abolished binding of these proteins to the BR3 sequence, significantly increased hIL-5 promoter activity in activated T cells.\nCONCLUSION: We suggest that Oct1, YY1, and octamer-like factors binding to the -90/-79 sequence within the proximal IL-5 promoter are involved in suppression of IL-5 transcription in T cells."}

genia-medco-coref

GENIAcorpus

metamap-sample

PubmedHPO

{"project":"PubmedHPO","denotations":[{"id":"T2","span":{"begin":258,"end":264},"obj":"HP_0002099"},{"id":"T1","span":{"begin":140,"end":152},"obj":"HP_0001880"}],"text":"Binding of YY1 and Oct1 to a novel element that downregulates expression of IL-5 in human T cells.\nBACKGROUND: IL-5 controls development of eosinophilia and has been shown to be involved in the pathogenesis of allergic diseases. In both atopic and nonatopic asthma, elevated IL-5 has been detected in peripheral blood and the airways. IL-5 is produced mainly by activated T cells, and its expression is regulated at the transcriptional level.\nOBJECTIVE: This study focuses on the functional analysis of the human IL-5 (hIL-5) promoter and characterization of cis -regulatory elements and transcription factors involved in the suppression of IL-5 transcription in T cells.\nMETHODS: Methods used in this study include DNase I footprint assays, electrophoretic mobility shift assays, and functional analysis by mammalian cell transfection involving deletion analysis and site-directed mutagenesis.\nRESULTS: We identified 5 protein binding regions (BRs) located within the proximal hIL-5 promoter. Functional analysis indicates that the BRs are involved in control of hIL-5 promoter activity. Two of these regions, BR3 and BR4 located at positions -102 to -73, have not previously been described as regulators of IL-5 expression in T cells. We show that the BR3 sequence contains a novel negative regulatory element located at positions -90 to -79 of the hIL-5 promoter, which binds Oct1, octamer-like, and YY1 nuclear factors. Substitution mutations, which abolished binding of these proteins to the BR3 sequence, significantly increased hIL-5 promoter activity in activated T cells.\nCONCLUSION: We suggest that Oct1, YY1, and octamer-like factors binding to the -90/-79 sequence within the proximal IL-5 promoter are involved in suppression of IL-5 transcription in T cells."}