PubMed:10359012 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":73,"end":112},"obj":"protein"},{"id":"T2","span":{"begin":114,"end":117},"obj":"protein"},{"id":"T3","span":{"begin":192,"end":224},"obj":"protein"},{"id":"T4","span":{"begin":226,"end":230},"obj":"protein"},{"id":"T5","span":{"begin":351,"end":389},"obj":"cell_line"},{"id":"T6","span":{"begin":427,"end":431},"obj":"protein"},{"id":"T7","span":{"begin":436,"end":440},"obj":"protein"},{"id":"T8","span":{"begin":508,"end":512},"obj":"protein"},{"id":"T9","span":{"begin":563,"end":567},"obj":"protein"},{"id":"T10","span":{"begin":605,"end":609},"obj":"protein"},{"id":"T11","span":{"begin":728,"end":767},"obj":"protein"},{"id":"T12","span":{"begin":769,"end":772},"obj":"protein"},{"id":"T13","span":{"begin":797,"end":801},"obj":"protein"},{"id":"T14","span":{"begin":887,"end":897},"obj":"cell_line"},{"id":"T15","span":{"begin":1024,"end":1032},"obj":"protein"},{"id":"T16","span":{"begin":1063,"end":1076},"obj":"protein"},{"id":"T17","span":{"begin":1118,"end":1128},"obj":"RNA"},{"id":"T18","span":{"begin":1178,"end":1181},"obj":"protein"}],"text":"Phorbol ester-induced mononuclear cell differentiation is blocked by the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059.\nThe purpose of this study was to evaluate whether the mitogen-activated protein kinase (MAPK) signaling pathway contributes to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mononuclear differentiation in the human myeloblastic leukemia ML-1 cells. Upon TPA treatment, the activity of ERK1 and ERK2 rapidly increased, with maximal induction between 1 and 3 h, while ERK2 protein levels remained constant. The activity of JNK1 was also significantly induced, with JNK1 protein levels increasing moderately during exposure to TPA. Treatment of cells with PD98059, a specific inhibitor of mitogen-activated protein kinase kinase (MEK), inhibited TPA-induced ERK2 activity. Furthermore, PD98059 completely blocked the TPA-induced differentiation of ML-1 cells, as assessed by a number of features associated with mononuclear differentiation including changes in morphology, nonspecific esterase activity, phagocytic ability, NADPH oxidase activity, mitochondrial respiration, and c-jun mRNA inducibility. We conclude that activation of the MEK/ERK signaling pathway is necessary for TPA-induced mononuclear cell differentiation."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"S2","span":{"begin":138,"end":390},"obj":"Sentence"},{"id":"S3","span":{"begin":391,"end":546},"obj":"Sentence"},{"id":"S4","span":{"begin":547,"end":670},"obj":"Sentence"},{"id":"S5","span":{"begin":671,"end":811},"obj":"Sentence"},{"id":"S6","span":{"begin":812,"end":1142},"obj":"Sentence"},{"id":"S7","span":{"begin":1143,"end":1266},"obj":"Sentence"}],"text":"Phorbol ester-induced mononuclear cell differentiation is blocked by the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059.\nThe purpose of this study was to evaluate whether the mitogen-activated protein kinase (MAPK) signaling pathway contributes to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mononuclear differentiation in the human myeloblastic leukemia ML-1 cells. Upon TPA treatment, the activity of ERK1 and ERK2 rapidly increased, with maximal induction between 1 and 3 h, while ERK2 protein levels remained constant. The activity of JNK1 was also significantly induced, with JNK1 protein levels increasing moderately during exposure to TPA. Treatment of cells with PD98059, a specific inhibitor of mitogen-activated protein kinase kinase (MEK), inhibited TPA-induced ERK2 activity. Furthermore, PD98059 completely blocked the TPA-induced differentiation of ML-1 cells, as assessed by a number of features associated with mononuclear differentiation including changes in morphology, nonspecific esterase activity, phagocytic ability, NADPH oxidase activity, mitochondrial respiration, and c-jun mRNA inducibility. We conclude that activation of the MEK/ERK signaling pathway is necessary for TPA-induced mononuclear cell differentiation."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":54},"obj":"NP"},{"id":"C2","span":{"begin":69,"end":136},"obj":"NP"},{"id":"C4","span":{"begin":347,"end":389},"obj":"NP"},{"id":"C3","span":{"begin":265,"end":389},"obj":"NP"},{"id":"C5","span":{"begin":695,"end":702},"obj":"NP"},{"id":"C6","span":{"begin":704,"end":773},"obj":"NP"},{"id":"C7","span":{"begin":825,"end":832},"obj":"NP"},{"id":"C9","span":{"begin":887,"end":897},"obj":"NP"},{"id":"C8","span":{"begin":852,"end":897},"obj":"NP"},{"id":"C10","span":{"begin":1221,"end":1265},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C1"},{"id":"R2","pred":"coref-ident","subj":"C5","obj":"C2"},{"id":"R3","pred":"coref-appos","subj":"C6","obj":"C5"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C5"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C4"},{"id":"R6","pred":"coref-ident","subj":"C8","obj":"C3"},{"id":"R7","pred":"coref-ident","subj":"C10","obj":"C8"}],"text":"Phorbol ester-induced mononuclear cell differentiation is blocked by the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059.\nThe purpose of this study was to evaluate whether the mitogen-activated protein kinase (MAPK) signaling pathway contributes to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mononuclear differentiation in the human myeloblastic leukemia ML-1 cells. Upon TPA treatment, the activity of ERK1 and ERK2 rapidly increased, with maximal induction between 1 and 3 h, while ERK2 protein levels remained constant. The activity of JNK1 was also significantly induced, with JNK1 protein levels increasing moderately during exposure to TPA. Treatment of cells with PD98059, a specific inhibitor of mitogen-activated protein kinase kinase (MEK), inhibited TPA-induced ERK2 activity. Furthermore, PD98059 completely blocked the TPA-induced differentiation of ML-1 cells, as assessed by a number of features associated with mononuclear differentiation including changes in morphology, nonspecific esterase activity, phagocytic ability, NADPH oxidase activity, mitochondrial respiration, and c-jun mRNA inducibility. We conclude that activation of the MEK/ERK signaling pathway is necessary for TPA-induced mononuclear cell differentiation."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":13},"obj":"other_organic_compound"},{"id":"T2","span":{"begin":73,"end":112},"obj":"protein_family_or_group"},{"id":"T3","span":{"begin":114,"end":117},"obj":"protein_family_or_group"},{"id":"T4","span":{"begin":129,"end":136},"obj":"other_organic_compound"},{"id":"T5","span":{"begin":192,"end":224},"obj":"protein_family_or_group"},{"id":"T6","span":{"begin":226,"end":230},"obj":"protein_family_or_group"},{"id":"T7","span":{"begin":265,"end":301},"obj":"other_organic_compound"},{"id":"T8","span":{"begin":303,"end":306},"obj":"other_organic_compound"},{"id":"T9","span":{"begin":351,"end":389},"obj":"cell_line"},{"id":"T10","span":{"begin":396,"end":399},"obj":"other_organic_compound"},{"id":"T11","span":{"begin":427,"end":431},"obj":"protein_molecule"},{"id":"T12","span":{"begin":436,"end":440},"obj":"protein_molecule"},{"id":"T13","span":{"begin":508,"end":512},"obj":"protein_molecule"},{"id":"T14","span":{"begin":563,"end":567},"obj":"protein_molecule"},{"id":"T15","span":{"begin":605,"end":609},"obj":"protein_molecule"},{"id":"T16","span":{"begin":666,"end":669},"obj":"other_organic_compound"},{"id":"T17","span":{"begin":695,"end":702},"obj":"other_organic_compound"},{"id":"T18","span":{"begin":728,"end":767},"obj":"protein_family_or_group"},{"id":"T19","span":{"begin":769,"end":772},"obj":"protein_family_or_group"},{"id":"T20","span":{"begin":785,"end":788},"obj":"other_organic_compound"},{"id":"T21","span":{"begin":797,"end":801},"obj":"protein_molecule"},{"id":"T22","span":{"begin":825,"end":832},"obj":"other_organic_compound"},{"id":"T23","span":{"begin":856,"end":859},"obj":"other_organic_compound"},{"id":"T24","span":{"begin":887,"end":897},"obj":"cell_line"},{"id":"T25","span":{"begin":1000,"end":1010},"obj":"other_name"},{"id":"T26","span":{"begin":1012,"end":1023},"obj":"other_name"},{"id":"T27","span":{"begin":1024,"end":1032},"obj":"protein_family_or_group"},{"id":"T28","span":{"begin":1043,"end":1061},"obj":"other_name"},{"id":"T29","span":{"begin":1063,"end":1076},"obj":"protein_molecule"},{"id":"T30","span":{"begin":1087,"end":1112},"obj":"other_name"},{"id":"T31","span":{"begin":1118,"end":1128},"obj":"RNA_molecule"},{"id":"T32","span":{"begin":1178,"end":1181},"obj":"protein_family_or_group"},{"id":"T33","span":{"begin":1221,"end":1224},"obj":"other_organic_compound"}],"text":"Phorbol ester-induced mononuclear cell differentiation is blocked by the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059.\nThe purpose of this study was to evaluate whether the mitogen-activated protein kinase (MAPK) signaling pathway contributes to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mononuclear differentiation in the human myeloblastic leukemia ML-1 cells. Upon TPA treatment, the activity of ERK1 and ERK2 rapidly increased, with maximal induction between 1 and 3 h, while ERK2 protein levels remained constant. The activity of JNK1 was also significantly induced, with JNK1 protein levels increasing moderately during exposure to TPA. Treatment of cells with PD98059, a specific inhibitor of mitogen-activated protein kinase kinase (MEK), inhibited TPA-induced ERK2 activity. Furthermore, PD98059 completely blocked the TPA-induced differentiation of ML-1 cells, as assessed by a number of features associated with mononuclear differentiation including changes in morphology, nonspecific esterase activity, phagocytic ability, NADPH oxidase activity, mitochondrial respiration, and c-jun mRNA inducibility. We conclude that activation of the MEK/ERK signaling pathway is necessary for TPA-induced mononuclear cell differentiation."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":370,"end":378},"obj":"HP_0001909"},{"id":"T2","span":{"begin":1087,"end":1100},"obj":"HP_0001427"}],"text":"Phorbol ester-induced mononuclear cell differentiation is blocked by the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059.\nThe purpose of this study was to evaluate whether the mitogen-activated protein kinase (MAPK) signaling pathway contributes to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mononuclear differentiation in the human myeloblastic leukemia ML-1 cells. Upon TPA treatment, the activity of ERK1 and ERK2 rapidly increased, with maximal induction between 1 and 3 h, while ERK2 protein levels remained constant. The activity of JNK1 was also significantly induced, with JNK1 protein levels increasing moderately during exposure to TPA. Treatment of cells with PD98059, a specific inhibitor of mitogen-activated protein kinase kinase (MEK), inhibited TPA-induced ERK2 activity. Furthermore, PD98059 completely blocked the TPA-induced differentiation of ML-1 cells, as assessed by a number of features associated with mononuclear differentiation including changes in morphology, nonspecific esterase activity, phagocytic ability, NADPH oxidase activity, mitochondrial respiration, and c-jun mRNA inducibility. We conclude that activation of the MEK/ERK signaling pathway is necessary for TPA-induced mononuclear cell differentiation."}