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PubMed:10318834 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-122 Sentence denotes Shared and unique determinants of the erythropoietin (EPO) receptor are important for binding EPO and EPO mimetic peptide.
T2 123-258 Sentence denotes We have shown previously that Phe93 in the extracellular domain of the erythropoietin (EPO) receptor (EPOR) is crucial for binding EPO.
T3 259-517 Sentence denotes Substitution of Phe93 with alanine resulted in a dramatic decrease in EPO binding to the Escherichia coli-expressed extracellular domain of the EPOR (EPO-binding protein or EBP) and no detectable binding to full-length mutant receptor expressed in COS cells.
T4 518-716 Sentence denotes Remarkably, Phe93 forms extensive contacts with a peptide ligand in the crystal structure of the EBP bound to an EPO-mimetic peptide (EMP1), suggesting that Phe93 is also important for EMP1 binding.
T5 717-880 Sentence denotes We used alanine substitution of EBP residues that contact EMP1 in the crystal structure to investigate the function of these residues in both EMP1 and EPO binding.
T6 881-1008 Sentence denotes The three largest hydrophobic contacts at Phe93, Met150, and Phe205 and a hydrogen bonding interaction at Thr151 were examined.
T7 1009-1225 Sentence denotes Our results indicate that Phe93 and Phe205 are important for both EPO and EMP1 binding, Met150 is not important for EPO binding but is critical for EMP1 binding, and Thr151 is not important for binding either ligand.
T8 1226-1480 Sentence denotes Thus, Phe93 and Phe205 are important binding determinants for both EPO and EMP1, even though these ligands share no sequence or structural homology, suggesting that these residues may represent a minimum epitope on the EPOR for productive ligand binding.

FSU-PRGE

Id Subject Object Predicate Lexical cue
T1 38-67 protein denotes erythropoietin (EPO) receptor
T2 94-97 protein denotes EPO
T3 102-105 protein denotes EPO
T4 194-223 protein denotes erythropoietin (EPO) receptor
T5 225-229 protein denotes EPOR
T6 254-257 protein denotes EPO
T7 329-332 protein denotes EPO
T8 403-407 protein denotes EPOR
T9 409-412 protein denotes EPO
T10 631-634 protein denotes EPO
T11 868-871 protein denotes EPO
T12 1075-1078 protein denotes EPO
T13 1125-1128 protein denotes EPO
T14 1293-1296 protein denotes EPO
T15 1445-1449 protein denotes EPOR

AIMed

Id Subject Object Predicate Lexical cue
T1 38-67 protein denotes erythropoietin (EPO) receptor
T2 94-97 protein denotes EPO
T3 102-121 protein denotes EPO mimetic peptide
T4 194-223 protein denotes erythropoietin (EPO) receptor
T5 225-229 protein denotes EPOR
T6 254-257 protein denotes EPO
T7 329-332 protein denotes EPO
T8 403-407 protein denotes EPOR
T9 409-428 protein denotes EPO-binding protein
T10 432-435 protein denotes EBP
T11 615-618 protein denotes EBP
T12 631-650 protein denotes EPO-mimetic peptide
T13 652-656 protein denotes EMP1
T14 703-707 protein denotes EMP1
T15 749-752 protein denotes EBP
T16 775-779 protein denotes EMP1
T17 859-863 protein denotes EMP1
T18 868-871 protein denotes EPO
T19 1075-1078 protein denotes EPO
T20 1083-1087 protein denotes EMP1
T21 1125-1128 protein denotes EPO
T22 1157-1161 protein denotes EMP1
T23 1293-1296 protein denotes EPO
T24 1301-1305 protein denotes EMP1
T25 1445-1449 protein denotes EPOR

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 348-364 OrganismTaxon denotes Escherichia coli 562

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 166-179 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576
T2 375-388 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576