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PubMed:10233888 JSONTXT

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jnlpba-st-training

Id Subject Object Predicate Lexical cue
T1 0-46 cell_line denotes Unicellular-unilineage erythropoietic cultures
T2 318-344 cell_type denotes progenitor/precursor cells
T3 440-479 cell_type denotes purified hematopoietic progenitor cells
T4 593-606 protein denotes growth factor
T5 608-610 protein denotes GF
T6 629-661 cell_type denotes cord blood (CB) progenitor cells
T7 724-741 cell_type denotes erythroid lineage
T8 887-912 cell_type denotes hematopoietic progenitors
T9 1100-1121 protein denotes reverse transcriptase
T10 1243-1252 protein denotes receptors
T11 1254-1280 protein denotes markers of differentiation
T12 1286-1307 protein denotes transcription factors
T13 1309-1313 protein denotes EKLF
T14 1315-1321 protein denotes GATA-1
T15 1323-1329 protein denotes GATA-2
T16 1331-1340 protein denotes p45 NF-E2
T17 1342-1346 protein denotes PU.1
T18 1352-1360 protein denotes SCL/Tal1
T19 1429-1442 cell_type denotes CD34(+) cells
T20 1453-1457 protein denotes CD34
T21 1459-1464 protein denotes c-kit
T22 1466-1470 protein denotes PU.1
T23 1476-1482 protein denotes GATA-2
T24 1503-1507 protein denotes CD36
T25 1509-1532 protein denotes erythropoietin receptor
T26 1534-1538 protein denotes EpoR
T27 1541-1549 protein denotes SCL/Tal1
T28 1551-1555 protein denotes EKLF
T29 1564-1570 protein denotes GATA-1
T30 1575-1589 protein denotes glyocophorin A
T31 1591-1594 protein denotes GPA
T32 1688-1692 protein denotes CD36
T33 1694-1698 protein denotes Tal1
T34 1700-1704 protein denotes EKLF
T35 1706-1711 protein denotes NF-E2
T36 1717-1723 protein denotes GATA-1
T37 1753-1757 protein denotes EpoR
T38 1799-1802 protein denotes GPA
T39 1828-1832 protein denotes CD34
T40 1834-1839 protein denotes c-kit
T41 1841-1845 protein denotes PU.1
T42 1851-1857 protein denotes GATA-2
T43 1949-1970 RNA denotes CD34 mRNA transcripts
T44 1974-2002 cell_line denotes sibling CD34(+)CD38(-) cells
T45 2022-2051 cell_line denotes unilineage erythroid cultures
T46 2126-2135 RNA denotes CD34 mRNA
T47 2141-2157 cell_type denotes progenitor cells
T48 2172-2205 cell_line denotes differentiating erythroid progeny
T49 2390-2426 cell_type denotes hematopoietic progenitors/precursors

pubmed-sentences-benchmark

Id Subject Object Predicate Lexical cue
S1 0-119 Sentence denotes Unicellular-unilineage erythropoietic cultures: molecular analysis of regulatory gene expression at sibling cell level.
S2 120-364 Sentence denotes In vitro studies on hematopoietic control mechanisms have been hampered by the heterogeneity of the analyzed cell populations, ie, lack of lineage specificity and developmental stage homogeneity of progenitor/precursor cells growing in culture.
S3 365-547 Sentence denotes We developed unicellular culture systems for unilineage differentiation of purified hematopoietic progenitor cells followed by daughter cell analysis at cellular and molecular level.
S4 548-1087 Sentence denotes In the culture system reported here, (1) the growth factor (GF) stimulus induces cord blood (CB) progenitor cells to proliferate and differentiate/mature exclusively along the erythroid lineage; (2) this erythropoietic wave is characterized by less than 4% apoptotic cells; (3) asymmetric divisions are virtually absent, ie, nonresponsive hematopoietic progenitors with no erythropoietic potential are forced into apoptosis; (4) the system is cell division controlled (cdc), ie, the number of divisions performed by each cell is monitored.
S5 1088-1411 Sentence denotes Single-cell reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was applied to this culture system to investigate gene expression of diverse receptors, markers of differentiation, and transcription factors (EKLF, GATA-1, GATA-2, p45 NF-E2, PU.1, and SCL/Tal1) at discrete stages of erythropoietic development.
S6 1412-1596 Sentence denotes Freshly isolated CD34(+) cells expressed CD34, c-kit, PU.1, and GATA-2 but did not express CD36, erythropoietin receptor (EpoR), SCL/Tal1, EKLF, NF-E2, GATA-1, or glyocophorin A (GPA).
S7 1597-1758 Sentence denotes In early to intermediate stages of erythroid differentiation we monitored the induction of CD36, Tal1, EKLF, NF-E2, and GATA-1 that preceeded expression of EpoR.
S8 1759-1886 Sentence denotes In late stages of erythroid maturation, GPA was upregulated, whereas CD34, c-kit, PU.1, and GATA-2 were barely or not detected.
S9 1887-2206 Sentence denotes In addition, competitive single-cell RT-PCR was used to assay CD34 mRNA transcripts in sibling CD34(+)CD38(-) cells differentiating in unilineage erythroid cultures: this analysis allowed us to semiquantitate the gradual downmodulation of CD34 mRNA from progenitor cells through their differentiating erythroid progeny.
S10 2207-2497 Sentence denotes It is concluded that this novel culture system, coupled with single-cell RT-PCR analysis, may eliminate the ambiguities intrinsic to molecular studies on heterogeneous populations of hematopoietic progenitors/precursors growing in culture, particularly in the initial stages of development.

genia-medco-coref

Id Subject Object Predicate Lexical cue
C1 195-245 NP denotes the heterogeneity of the analyzed cell populations
C3 356-363 NP denotes culture
C2 251-363 NP denotes lack of lineage specificity and developmental stage homogeneity of progenitor/precursor cells growing in culture
C4 551-583 NP denotes the culture system reported here
C5 977-987 NP denotes the system
C6 1088-1165 NP denotes Single-cell reverse transcriptase-polymerase chain reaction (RT-PCR) analysis
C7 1181-1200 NP denotes this culture system
C8 1453-1482 NP denotes CD34, c-kit, PU.1, and GATA-2
C9 1503-1507 NP denotes CD36
C10 1509-1539 NP denotes erythropoietin receptor (EpoR)
C11 1551-1555 NP denotes EKLF
C12 1557-1562 NP denotes NF-E2
C13 1564-1570 NP denotes GATA-1
C14 1575-1595 NP denotes glyocophorin A (GPA)
C16 1688-1692 NP denotes CD36
C17 1700-1704 NP denotes EKLF
C18 1706-1711 NP denotes NF-E2
C19 1717-1723 NP denotes GATA-1
C15 1671-1723 NP denotes the induction of CD36, Tal1, EKLF, NF-E2, and GATA-1
C20 1724-1728 NP denotes that
C21 1753-1757 NP denotes EpoR
C22 1799-1802 NP denotes GPA
C23 1828-1857 NP denotes CD34, c-kit, PU.1, and GATA-2
C24 1900-1930 NP denotes competitive single-cell RT-PCR
C25 2053-2066 NP denotes this analysis
C26 2141-2157 NP denotes progenitor cells
C27 2166-2171 NP denotes their
C28 2228-2253 NP denotes this novel culture system
C29 2268-2295 NP denotes single-cell RT-PCR analysis
C30 2438-2445 NP denotes culture
R1 C2 C1 coref-ident lack of lineage specificity and developmental stage homogeneity of progenitor/precursor cells growing in culture,the heterogeneity of the analyzed cell populations
R2 C5 C4 coref-ident the system,the culture system reported here
R3 C7 C5 coref-ident this culture system,the system
R4 C16 C9 coref-ident CD36,CD36
R5 C17 C11 coref-ident EKLF,EKLF
R6 C18 C12 coref-ident NF-E2,NF-E2
R7 C19 C13 coref-ident GATA-1,GATA-1
R8 C20 C15 coref-relat that,"the induction of CD36, Tal1, EKLF, NF-E2, and GATA-1"
R9 C21 C10 coref-ident EpoR,erythropoietin receptor (EpoR)
R10 C22 C14 coref-ident GPA,glyocophorin A (GPA)
R11 C23 C8 coref-ident "CD34, c-kit, PU.1, and GATA-2","CD34, c-kit, PU.1, and GATA-2"
R12 C25 C24 coref-ident this analysis,competitive single-cell RT-PCR
R13 C27 C26 coref-pron their,progenitor cells
R14 C28 C7 coref-ident this novel culture system,this culture system
R15 C29 C6 coref-ident single-cell RT-PCR analysis,Single-cell reverse transcriptase-polymerase chain reaction (RT-PCR) analysis
R16 C30 C3 coref-ident culture,culture

GENIAcorpus

Id Subject Object Predicate Lexical cue
T1 0-46 cell_line denotes Unicellular-unilineage erythropoietic cultures
T2 70-96 other_name denotes regulatory gene expression
T3 100-118 other_name denotes sibling cell level
T4 140-172 other_name denotes hematopoietic control mechanisms
T5 283-314 other_name denotes developmental stage homogeneity
T6 318-344 cell_type denotes progenitor/precursor cells
T7 410-436 other_name denotes unilineage differentiation
T8 440-462 cell_type denotes purified hematopoietic
T9 463-479 cell_type denotes progenitor cells
T10 593-606 protein_family_or_group denotes growth factor
T11 608-610 protein_family_or_group denotes GF
T12 629-644 cell_type denotes cord blood (CB)
T13 645-661 cell_type denotes progenitor cells
T14 724-741 cell_type denotes erythroid lineage
T15 752-771 other_name denotes erythropoietic wave
T16 826-846 other_name denotes asymmetric divisions
T17 887-912 cell_type denotes hematopoietic progenitors
T18 962-971 other_name denotes apoptosis
T19 1088-1099 other_name denotes Single-cell
T20 1100-1121 protein_molecule denotes reverse transcriptase
T21 1149-1155 other_name denotes RT-PCR
T22 1216-1231 other_name denotes gene expression
T23 1243-1252 protein_family_or_group denotes receptors
T24 1254-1280 protein_family_or_group denotes markers of differentiation
T25 1286-1307 protein_family_or_group denotes transcription factors
T26 1309-1313 protein_molecule denotes EKLF
T27 1315-1321 protein_molecule denotes GATA-1
T28 1323-1329 protein_molecule denotes GATA-2
T29 1331-1340 protein_molecule denotes p45 NF-E2
T30 1342-1346 protein_molecule denotes PU.1
T31 1352-1360 protein_molecule denotes SCL/Tal1
T32 1384-1410 other_name denotes erythropoietic development
T33 1429-1442 cell_type denotes CD34(+) cells
T34 1453-1457 protein_molecule denotes CD34
T35 1459-1464 protein_molecule denotes c-kit
T36 1466-1470 protein_molecule denotes PU.1
T37 1476-1482 protein_molecule denotes GATA-2
T38 1503-1507 protein_molecule denotes CD36
T39 1509-1523 protein_family_or_group denotes erythropoietin
T40 1534-1538 protein_family_or_group denotes EpoR
T41 1541-1549 protein_molecule denotes SCL/Tal1
T42 1551-1555 protein_molecule denotes EKLF
T43 1564-1570 protein_molecule denotes GATA-1
T44 1575-1589 protein_molecule denotes glyocophorin A
T45 1591-1594 protein_molecule denotes GPA
T46 1632-1657 other_name denotes erythroid differentiation
T47 1688-1692 protein_molecule denotes CD36
T48 1694-1698 protein_molecule denotes Tal1
T49 1700-1704 protein_molecule denotes EKLF
T50 1706-1711 protein_molecule denotes NF-E2
T51 1717-1723 protein_molecule denotes GATA-1
T52 1753-1757 protein_family_or_group denotes EpoR
T53 1799-1802 protein_molecule denotes GPA
T54 1828-1832 protein_molecule denotes CD34
T55 1834-1839 protein_molecule denotes c-kit
T56 1841-1845 protein_molecule denotes PU.1
T57 1851-1857 protein_molecule denotes GATA-2
T58 1924-1930 other_name denotes RT-PCR
T59 1949-1953 protein_molecule denotes CD34
T60 1974-1981 cell_line denotes sibling
T61 1982-1986 protein_molecule denotes CD34
T62 2022-2051 cell_line denotes unilineage erythroid cultures
T63 2126-2130 protein_molecule denotes CD34
T64 2141-2157 cell_type denotes progenitor cells
T65 2172-2205 cell_line denotes differentiating erythroid progeny
T66 2280-2286 other_name denotes RT-PCR
T67 2361-2386 other_name denotes heterogeneous populations
T68 2390-2415 cell_type denotes hematopoietic progenitors