PubMed:10233085 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10233085","sourcedb":"PubMed","sourceid":"10233085","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10233085","text":"Electronic spectra of PS I mutants: the peripheral subunits do not bind red chlorophylls in Synechocystis sp. PCC 6803.\nSteady-state fluorescence and absorption spectra have been obtained in the Qy spectral region (690-780 nm and 600-750 nm, respectively) for several subunit-deficient photosystem I mutants from the cyanobacterium Synechocystis sp. PCC 6803. The 77 K fluorescence spectra of the wild-type and subunit-deficient mutant photosystem I particles are all very similar, peaking at approximately 720 nm with essentially the same excitation spectrum. Because emission from far-red chlorophylls absorbing near 708 nm dominates low-temperature fluorescence in Synechocystis sp., these pigments are not coordinated to any the subunits PsaF, Psa I, PsaJ, PsaK, PsaL, or psaM. The room temperature (wild-type-mutant) absorption difference spectra for trimeric mutants lacking the PsaF/J, PsaK, and PsaM subunits suggest that these mutants are deficient in core antenna chlorophylls (Chls) absorbing near 685, 670, 675, and 700 nm, respectively. The absorption difference spectrum for the PsaF/J/I/L-deficient photosystem I complexes at 5 K reveals considerably more structure than the room-temperature spectrum. The integrated absorbance difference spectra (when normalized to the total PS I Qy spectral area) are comparable to the fractions of Chls bound by the respective (groups of) subunits, according to the 4-A density map of PS I from Synechococcus elongatus. The spectrum of the monomeric PsaL-deficient mutant suggests that this subunit may bind pigments absorbing near 700 nm.","tracks":[{"project":"CyanoBase","denotations":[{"id":"T1","span":{"begin":742,"end":746},"obj":"protein"},{"id":"T2","span":{"begin":748,"end":753},"obj":"protein"},{"id":"T3","span":{"begin":755,"end":759},"obj":"protein"},{"id":"T4","span":{"begin":767,"end":771},"obj":"protein"},{"id":"T5","span":{"begin":776,"end":780},"obj":"protein"},{"id":"T6","span":{"begin":885,"end":889},"obj":"protein"},{"id":"T7","span":{"begin":890,"end":891},"obj":"protein"},{"id":"T8","span":{"begin":903,"end":907},"obj":"protein"},{"id":"T9","span":{"begin":1093,"end":1097},"obj":"protein"},{"id":"T10","span":{"begin":1098,"end":1099},"obj":"protein"},{"id":"T11","span":{"begin":1100,"end":1101},"obj":"protein"},{"id":"T12","span":{"begin":1102,"end":1103},"obj":"protein"},{"id":"T13","span":{"begin":1502,"end":1506},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"CyanoBase"},{"subj":"T2","pred":"source","obj":"CyanoBase"},{"subj":"T3","pred":"source","obj":"CyanoBase"},{"subj":"T4","pred":"source","obj":"CyanoBase"},{"subj":"T5","pred":"source","obj":"CyanoBase"},{"subj":"T6","pred":"source","obj":"CyanoBase"},{"subj":"T7","pred":"source","obj":"CyanoBase"},{"subj":"T8","pred":"source","obj":"CyanoBase"},{"subj":"T9","pred":"source","obj":"CyanoBase"},{"subj":"T10","pred":"source","obj":"CyanoBase"},{"subj":"T11","pred":"source","obj":"CyanoBase"},{"subj":"T12","pred":"source","obj":"CyanoBase"},{"subj":"T13","pred":"source","obj":"CyanoBase"}]},{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":110,"end":113},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":350,"end":353},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":748,"end":751},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":988,"end":992},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":1350,"end":1354},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0004974"},{"id":"A2","pred":"mondo_id","subj":"T1","obj":"0022673"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0004974"},{"id":"A4","pred":"mondo_id","subj":"T3","obj":"0022673"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0008401"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0008966"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0008966"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T5","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T6","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T7","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"CyanoBase","color":"#ec93c1","default":true},{"id":"PMID_GLOBAL","color":"#93dbec"}]}]}}