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PubMed:10224156 JSONTXT

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jnlpba-st-training

Id Subject Object Predicate Lexical cue
T1 0-21 protein denotes Transcription factors
T2 22-25 protein denotes Sp1
T3 30-34 protein denotes AP-2
T4 56-77 protein denotes acid sphingomyelinase
T5 127-161 cell_line denotes human monocytic leukemia cell line
T6 162-167 cell_line denotes THP-1
T7 380-396 protein denotes lysosomal enzyme
T8 397-418 protein denotes acid sphingomyelinase
T9 514-525 cell_line denotes THP-1 cells
T10 556-584 cell_type denotes human mononuclear phagocytes
T11 611-616 protein denotes RNase
T12 962-985 DNA denotes 5' deletion derivatives
T13 993-1019 DNA denotes upstream regulatory region
T14 1075-1092 DNA denotes promoter elements
T15 1145-1167 DNA denotes PMA responsive element
T16 1202-1227 DNA denotes -319 and -219 bp upstream
T17 1278-1318 DNA denotes transcription factor expression plasmids
T18 1323-1327 protein denotes AP-2
T19 1332-1335 protein denotes Sp1
T20 1410-1423 DNA denotes binding sites
T21 1583-1586 protein denotes Sp1
T22 1591-1595 protein denotes AP-2
T23 1684-1695 cell_line denotes THP-1 cells
T24 1790-1807 cell_line denotes PMA-treated cells
T25 1882-1903 protein denotes transcription factors
T26 1904-1908 protein denotes AP-2
T27 1913-1916 protein denotes Sp1

pubmed-sentences-benchmark

Id Subject Object Predicate Lexical cue
S1 0-111 Sentence denotes Transcription factors Sp1 and AP-2 mediate induction of acid sphingomyelinase during monocytic differentiation.
S2 112-332 Sentence denotes Cells from the human monocytic leukemia cell line THP-1 differentiate towards a macrophage-like phenotype when stimulated with phorbol 12-myristate -13- acetate (PMA), 1,25-dihydroxy-vitamin D3, and various other agents.
S3 333-585 Sentence denotes We demonstrate here that the expression of the lysosomal enzyme acid sphingomyelinase (ASM; E.C. 3.1.4.12) is induced during this process and is strongly elevated in differentiated THP-1 cells, as well as in differentiated human mononuclear phagocytes.
S4 586-832 Sentence denotes Using Northern blotting, RNase protection assay, and nuclear run-on analyses, we show that the up-regulation of ASM expression is regulated mainly at the level of transcription and that new protein synthesis is required for enhanced ASM activity.
S5 833-949 Sentence denotes This cell-type specific induction by PMA treatment was further investigated with respect to transcriptional control.
S6 950-1142 Sentence denotes A series of 5' deletion derivatives of the upstream regulatory region were used in transient transfection assays to identify promoter elements required for basal and inducible gene expression.
S7 1143-1459 Sentence denotes A PMA responsive element was localized to a region between -319 and -219 bp upstream of the initiation codon and co-transfections with transcription factor expression plasmids for AP-2 and Sp1 resulted in augmented ASM promoter activity, which was abolished when the binding sites for these two factors were deleted.
S8 1460-1596 Sentence denotes Using electrophoretic mobility shift assays and supershift assays we demonstrate that this region is specifically bound by Sp1 and AP-2.
S9 1597-1696 Sentence denotes These factors are present in nuclear extracts prepared from both induced and uninduced THP-1 cells.
S10 1697-1818 Sentence denotes However, the intensity of the complex formed appeared to increase when nuclear extracts from PMA-treated cells were used.
S11 1819-2020 Sentence denotes From these studies, we conclude that a concerted action of the transcription factors AP-2 and Sp1 is essential for the up -regulation of ASM expression during the process of macrophage differentiation.

genia-medco-coref

Id Subject Object Predicate Lexical cue
C1 0-34 NP denotes Transcription factors Sp1 and AP-2
C2 698-712 NP denotes ASM expression
C4 1323-1335 NP denotes AP-2 and Sp1
C5 1348-1379 NP denotes augmented ASM promoter activity
C6 1381-1386 NP denotes which
C7 1428-1445 NP denotes these two factors
C3 1185-1458 NP denotes a region between -319 and -219 bp upstream of the initiation codon and co-transfections with transcription factor expression plasmids for AP-2 and Sp1 resulted in augmented ASM promoter activity, which was abolished when the binding sites for these two factors were deleted
C8 1546-1557 NP denotes this region
C9 1583-1595 NP denotes Sp1 and AP-2
C10 1597-1610 NP denotes These factors
C11 1878-1916 NP denotes the transcription factors AP-2 and Sp1
C12 1956-1970 NP denotes ASM expression
R1 C4 C1 coref-ident AP-2 and Sp1,Transcription factors Sp1 and AP-2
R2 C6 C5 coref-relat which,augmented ASM promoter activity
R3 C7 C4 coref-ident these two factors,AP-2 and Sp1
R4 C8 C3 coref-ident this region,"a region between -319 and -219 bp upstream of the initiation codon and co-transfections with transcription factor expression plasmids for AP-2 and Sp1 resulted in augmented ASM promoter activity, which was abolished when the binding sites for these two factors were deleted"
R5 C9 C7 coref-ident Sp1 and AP-2,these two factors
R6 C10 C9 coref-ident These factors,Sp1 and AP-2
R7 C11 C10 coref-ident the transcription factors AP-2 and Sp1,These factors
R8 C12 C2 coref-ident ASM expression,ASM expression

GENIAcorpus

Id Subject Object Predicate Lexical cue
T1 0-21 protein_family_or_group denotes Transcription factors
T2 22-25 protein_molecule denotes Sp1
T3 30-34 protein_molecule denotes AP-2
T4 56-77 protein_family_or_group denotes acid sphingomyelinase
T5 85-110 other_name denotes monocytic differentiation
T6 127-161 cell_line denotes human monocytic leukemia cell line
T7 162-167 cell_line denotes THP-1
T8 239-272 other_organic_compound denotes phorbol 12-myristate -13- acetate
T9 274-277 other_organic_compound denotes PMA
T10 280-305 lipid denotes 1,25-dihydroxy-vitamin D3
T11 380-396 protein_family_or_group denotes lysosomal enzyme
T12 397-418 protein_family_or_group denotes acid sphingomyelinase
T13 514-525 cell_line denotes THP-1 cells
T14 556-584 cell_type denotes human mononuclear phagocytes
T15 592-609 other_name denotes Northern blotting
T16 611-616 protein_molecule denotes RNase
T17 639-662 other_name denotes nuclear run-on analyses
T18 698-712 other_name denotes ASM expression
T19 776-793 other_name denotes protein synthesis
T20 819-831 other_name denotes ASM activity
T21 870-873 other_organic_compound denotes PMA
T22 925-948 other_name denotes transcriptional control
T23 962-985 DNA_family_or_group denotes 5' deletion derivatives
T24 993-1019 DNA_domain_or_region denotes upstream regulatory region
T25 1033-1062 other_name denotes transient transfection assays
T26 1075-1092 DNA_family_or_group denotes promoter elements
T27 1126-1141 other_name denotes gene expression
T28 1145-1148 other_organic_compound denotes PMA
T29 1202-1227 DNA_domain_or_region denotes -319 and -219 bp upstream
T30 1278-1318 DNA_family_or_group denotes transcription factor expression plasmids
T31 1323-1327 protein_molecule denotes AP-2
T32 1332-1335 protein_molecule denotes Sp1
T33 1358-1379 other_name denotes ASM promoter activity
T34 1410-1423 DNA_domain_or_region denotes binding sites
T35 1466-1503 other_name denotes electrophoretic mobility shift assays
T36 1508-1525 other_name denotes supershift assays
T37 1583-1586 protein_molecule denotes Sp1
T38 1591-1595 protein_molecule denotes AP-2
T39 1626-1642 cell_component denotes nuclear extracts
T40 1684-1695 cell_line denotes THP-1 cells
T41 1768-1784 cell_component denotes nuclear extracts
T42 1790-1793 other_organic_compound denotes PMA
T43 1882-1903 protein_family_or_group denotes transcription factors
T44 1904-1908 protein_molecule denotes AP-2
T45 1913-1916 protein_molecule denotes Sp1
T46 1938-1952 other_name denotes up -regulation
T47 1956-1970 other_name denotes ASM expression
T48 1993-2019 other_name denotes macrophage differentiation

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 143-151 HP_0001909 denotes leukemia