PubMed:10223563 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10223563","sourcedb":"PubMed","sourceid":"10223563","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10223563","text":"The K-ras mutation pattern in pancreatic ductal adenocarcinoma usually is identical to that in associated normal, hyperplastic, and metaplastic ductal epithelium.\nBACKGROUND: Hyperplastic ductal lesions of the pancreas are believed to represent precursors of ductal adenocarcinoma. The most frequent mutation in manifest ductal carcinoma of the pancreas is the K-ras mutation at codon 12. The frequency and significance of this mutation in precursor lesions are a matter of controversy.\nMETHODS: The study included 35 resection specimens of ductal adenocarcinoma of the head of the pancreas and 3 noncancerous, noninflammatory pancreases. Ductal lesions were classified according to established criteria. Single cells from these lesions were microdissected and analyzed by the denaturing gradient gel electrophoresis polymerase chain reaction method.\nRESULTS: All primary adenocarcinomas showed a K-ras mutation at codon 12 (25 cases with GAT, 7 cases with GTT, and 3 cases with CGT). One hundred and six of 364 ductal lesions were positive for the mutation. The highest relative percentage (53%) occurred in adenomatoid hyperplasia, followed by 36% in papillary hyperplasia, 26% in mucinous hypertrophy, and 14% in squamous metaplasia. With only two exceptions the mutation pattern of the ductal lesions and that of the corresponding primary tumor were identical. Twenty-one samples from normal ducts (17%) also harbored the K-ras mutation, as did 3 lesions from noncancerous specimens.\nCONCLUSIONS: K-ras mutations are common events in normal, hyperplastic, metaplastic, and neoplastic pancreatic ductal cells. Because K-ras mutations frequently, although not exclusively, are related to mucinous differentiation of pancreatic cells, this mutation may not cause but only promote mucinous differentiation. The prevalence of a certain mutation pattern in nonneoplastic and neoplastic ductal cells in an individual pancreas suggests the dominance of one carcinogenic factor.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":939,"end":942},"obj":"gene:10249"},{"id":"T1","span":{"begin":872,"end":887},"obj":"disease:C0001418"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"10223563-7#79#82#gene10249","span":{"begin":939,"end":942},"obj":"gene10249"},{"id":"10223563-7#119#122#gene7368","span":{"begin":979,"end":982},"obj":"gene7368"},{"id":"10223563-7#12#27#diseaseC0001418","span":{"begin":872,"end":887},"obj":"diseaseC0001418"}],"relations":[{"id":"79#82#gene1024912#27#diseaseC0001418","pred":"associated_with","subj":"10223563-7#79#82#gene10249","obj":"10223563-7#12#27#diseaseC0001418"},{"id":"119#122#gene736812#27#diseaseC0001418","pred":"associated_with","subj":"10223563-7#119#122#gene7368","obj":"10223563-7#12#27#diseaseC0001418"}],"attributes":[{"subj":"10223563-7#79#82#gene10249","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10223563-7#119#122#gene7368","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10223563-7#12#27#diseaseC0001418","pred":"source","obj":"DisGeNET5_gene_disease"}]},{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":175,"end":486},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":496,"end":850},"obj":"METHODS"},{"id":"T3","span":{"begin":860,"end":1487},"obj":"RESULTS"},{"id":"T4","span":{"begin":1501,"end":1973},"obj":"CONCLUSIONS"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T3","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T4","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#93a9ec","default":true},{"id":"DisGeNET5_gene_disease","color":"#c3ec93"},{"id":"PubMed_Structured_Abstracts","color":"#ec93dd"}]}]}}