| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-102 |
Sentence |
denotes |
cumA, a gene encoding a multicopper oxidase, is involved in Mn2+ oxidation in Pseudomonas putida GB-1. |
| T2 |
103-163 |
Sentence |
denotes |
Pseudomonas putida GB-1-002 catalyzes the oxidation of Mn2+. |
| T3 |
164-339 |
Sentence |
denotes |
Nucleotide sequence analysis of the transposon insertion site of a nonoxidizing mutant revealed a gene (designated cumA) encoding a protein homologous to multicopper oxidases. |
| T4 |
340-453 |
Sentence |
denotes |
Addition of Cu2+ increased the Mn2+-oxidizing activity of the P. putida wild type by a factor of approximately 5. |
| T5 |
454-535 |
Sentence |
denotes |
The growth rates of the wild type and the mutant were not affected by added Cu2+. |
| T6 |
536-614 |
Sentence |
denotes |
A second open reading frame (designated cumB) is located downstream from cumA. |
| T7 |
615-671 |
Sentence |
denotes |
Both cumA and cumB probably are part of a single operon. |
| T8 |
672-789 |
Sentence |
denotes |
The translation product of cumB was homologous (level of identity, 45%) to that of orf74 of Bradyrhizobium japonicum. |
| T9 |
790-869 |
Sentence |
denotes |
A mutation in orf74 resulted in an extended lag phase and lower cell densities. |
| T10 |
870-1003 |
Sentence |
denotes |
Similar growth-related observations were made for the cumA mutant, suggesting that the cumA mutation may have a polar effect on cumB. |
| T11 |
1004-1065 |
Sentence |
denotes |
This was confirmed by site-specific gene replacement in cumB. |
| T12 |
1066-1175 |
Sentence |
denotes |
The cumB mutation did not affect the Mn2+-oxidizing ability of the organism but resulted in decreased growth. |
| T13 |
1176-1344 |
Sentence |
denotes |
In summary, our data indicate that the multicopper oxidase CumA is involved in the oxidation of Mn2+ and that CumB is required for optimal growth of P. putida GB-1-002. |
