PubMed:10089131 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10089131","sourcedb":"PubMed","sourceid":"10089131","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10089131","text":"Gene expression during differentiation of human dendritic cells from cord blood cd34 stem cells.\nHuman cord blood CD34(+)stem cells were allowed to differentiate in the presence of cytokines stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor alpha (TNF-alpha) into functional CD1a+dendritic cells (DC). A maximum of 1.9 x 10(6) CD1a+ cells were separated from the cells generated from 1.2 x 10(6) CD34(+) stem cells from an individual donor. The percentage of CD1a+cells separated rose to a maximum of 27% at day 11 and fell to 8% at 21 days. Reverse transcription-polymerase chain reaction analysis showed that interleukin 2 receptor, interleukin 3 receptor, interleukin 6 receptor, interleukin 12 receptor (IL-12R) and signal transducer and activator of transcription (STAT) 3, STAT 4 mRNA was expressed in all CD1a+cell populations throughout and appears to be constitutive. Expression of IL-12RmRNA was unexpected in CD1a+DC normally considered to be of myeloid lineage. Expression of interleukin 12 (IL-12) p40 subunit mRNA was not detected. Intermittent expression of the IL-12p35 subunit and IL-4R mRNA suggested that gene expression is inducible, but not obviously correlated with progressive DC development. Expression of mRNA for a spectrum of cytokine receptors indicates that CD1a+DC have the potential to respond to a variety of maturational signals.","tracks":[{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":80,"end":84},"obj":"protein"},{"id":"T2","span":{"begin":114,"end":118},"obj":"protein"},{"id":"T3","span":{"begin":181,"end":190},"obj":"protein"},{"id":"T4","span":{"begin":191,"end":207},"obj":"protein"},{"id":"T5","span":{"begin":209,"end":212},"obj":"protein"},{"id":"T6","span":{"begin":215,"end":263},"obj":"protein"},{"id":"T7","span":{"begin":265,"end":271},"obj":"protein"},{"id":"T8","span":{"begin":277,"end":305},"obj":"protein"},{"id":"T9","span":{"begin":307,"end":316},"obj":"protein"},{"id":"T10","span":{"begin":334,"end":338},"obj":"protein"},{"id":"T11","span":{"begin":386,"end":390},"obj":"protein"},{"id":"T12","span":{"begin":455,"end":459},"obj":"protein"},{"id":"T13","span":{"begin":518,"end":522},"obj":"protein"},{"id":"T14","span":{"begin":670,"end":692},"obj":"protein"},{"id":"T15","span":{"begin":694,"end":716},"obj":"protein"},{"id":"T16","span":{"begin":718,"end":740},"obj":"protein"},{"id":"T17","span":{"begin":742,"end":765},"obj":"protein"},{"id":"T18","span":{"begin":767,"end":773},"obj":"protein"},{"id":"T19","span":{"begin":779,"end":836},"obj":"protein"},{"id":"T20","span":{"begin":838,"end":844},"obj":"protein"},{"id":"T21","span":{"begin":871,"end":875},"obj":"protein"},{"id":"T22","span":{"begin":950,"end":960},"obj":"protein"},{"id":"T23","span":{"begin":979,"end":983},"obj":"protein"},{"id":"T24","span":{"begin":1047,"end":1073},"obj":"protein"},{"id":"T25","span":{"begin":1136,"end":1144},"obj":"protein"},{"id":"T26","span":{"begin":1157,"end":1162},"obj":"protein"},{"id":"T27","span":{"begin":1312,"end":1320},"obj":"protein"},{"id":"T28","span":{"begin":1346,"end":1350},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"FSU-PRGE"},{"subj":"T2","pred":"source","obj":"FSU-PRGE"},{"subj":"T3","pred":"source","obj":"FSU-PRGE"},{"subj":"T4","pred":"source","obj":"FSU-PRGE"},{"subj":"T5","pred":"source","obj":"FSU-PRGE"},{"subj":"T6","pred":"source","obj":"FSU-PRGE"},{"subj":"T7","pred":"source","obj":"FSU-PRGE"},{"subj":"T8","pred":"source","obj":"FSU-PRGE"},{"subj":"T9","pred":"source","obj":"FSU-PRGE"},{"subj":"T10","pred":"source","obj":"FSU-PRGE"},{"subj":"T11","pred":"source","obj":"FSU-PRGE"},{"subj":"T12","pred":"source","obj":"FSU-PRGE"},{"subj":"T13","pred":"source","obj":"FSU-PRGE"},{"subj":"T14","pred":"source","obj":"FSU-PRGE"},{"subj":"T15","pred":"source","obj":"FSU-PRGE"},{"subj":"T16","pred":"source","obj":"FSU-PRGE"},{"subj":"T17","pred":"source","obj":"FSU-PRGE"},{"subj":"T18","pred":"source","obj":"FSU-PRGE"},{"subj":"T19","pred":"source","obj":"FSU-PRGE"},{"subj":"T20","pred":"source","obj":"FSU-PRGE"},{"subj":"T21","pred":"source","obj":"FSU-PRGE"},{"subj":"T22","pred":"source","obj":"FSU-PRGE"},{"subj":"T23","pred":"source","obj":"FSU-PRGE"},{"subj":"T24","pred":"source","obj":"FSU-PRGE"},{"subj":"T25","pred":"source","obj":"FSU-PRGE"},{"subj":"T26","pred":"source","obj":"FSU-PRGE"},{"subj":"T27","pred":"source","obj":"FSU-PRGE"},{"subj":"T28","pred":"source","obj":"FSU-PRGE"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":277,"end":283},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"FSU-PRGE","color":"#acec93","default":true},{"id":"PubmedHPO","color":"#ec93c6"}]}]}}