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PubMed:10082137 JSONTXT

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FSU-PRGE

Id Subject Object Predicate Lexical cue
T1 23-44 protein denotes alpha1(I) procollagen
T2 118-134 protein denotes interferon-gamma
T3 136-152 protein denotes Interferon-gamma
T4 154-163 protein denotes IFN-gamma
T5 184-192 protein denotes cytokine
T6 362-383 protein denotes alpha1(I) procollagen
T7 390-396 protein denotes COL1A1
T8 424-433 protein denotes IFN-gamma
T9 459-468 protein denotes IFN-gamma
T10 506-541 protein denotes interferon regulated factor (IRF)-1
T11 603-624 protein denotes alpha1(I) procollagen
T12 763-768 protein denotes IRF-1
T13 892-898 protein denotes COL1A1
T14 960-966 protein denotes COL1A1
T15 976-1009 protein denotes chloramphenicol acetyltransferase
T16 1034-1043 protein denotes IFN-gamma
T17 1125-1133 protein denotes TGF-beta
T18 1153-1162 protein denotes IFN-gamma
T19 1196-1204 protein denotes TGF-beta
T20 1294-1303 protein denotes IFN-alpha
T21 1308-1316 protein denotes IFN-beta
T22 1375-1384 protein denotes IFN-alpha
T23 1420-1429 protein denotes IFN-gamma
T24 1498-1504 protein denotes COL1A1
T25 1542-1551 protein denotes IFN-gamma
T26 1654-1657 protein denotes Sp1
T27 1659-1662 protein denotes Sp3
T28 1668-1672 protein denotes NF-1
T29 1717-1726 protein denotes IFN-gamma
T30 1759-1768 protein denotes IFN-gamma
T31 1867-1876 protein denotes IFN-gamma
T32 2061-2070 protein denotes IFN-gamma
T33 2088-2097 protein denotes IFN-gamma
T34 2195-2204 protein denotes IFN-gamma
T35 2214-2220 protein denotes COL1A1
T36 2315-2320 protein denotes IRF-1
T37 2490-2499 protein denotes IFN-gamma

PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 136-316 DRI_Background denotes Interferon-gamma (IFN-gamma), a multifunctional cytokine produced by activated Th1 lymphocytes, exerts potent effects on the extracellular matrix by regulating fibroblast function.
T2 317-434 DRI_Approach denotes In this study, we examined the modulation of alpha1(I) procollagen gene (COL1A1) expression by recombinant IFN-gamma.
T3 435-691 DRI_Outcome denotes The results showed that IFN-gamma stimulated the rapid accumulation of interferon regulated factor (IRF)-1 mRNA, followed by a delayed and dose-dependent inhibition of alpha1(I) procollagen mRNA expression in skin fibroblasts from several different donors.
T4 692-798 DRI_Outcome denotes The inhibitory response was abrogated in fibroblasts stably expressing IRF-1 in the antisense orientation.
T5 799-915 DRI_Outcome denotes A marked decrease in the amount of heterogeneous nuclear pre-mRNA preceded the inhibition of COL1A1 mRNA expression.
T6 916-959 DRI_Background denotes In fibroblasts transiently transfected with
T7 992-1134 DRI_Background denotes acetyltransferase reporter gene plasmids, IFN-gamma selectively inhibited promoter activity and abrogated its stimulation induced by TGF-beta.
T8 1135-1293 DRI_Challenge denotes The inhibition by IFN-gamma was not due to downregulation of TGF-beta receptor mRNA expression in the fibroblasts or decreased ligand binding to the receptor.
T9 1294-1430 DRI_Approach denotes IFN-alpha and IFN-beta by themselves had little effect on promoter activity, but IFN-alpha augmented the inhibitory effect of IFN-gamma.
T10 1431-1569 DRI_Approach denotes Using a series of 5' deletion constructs, a proximal region of the COL1A1 promoter was shown to function as an IFN-gamma response element.
T11 1570-1745 DRI_Approach denotes This region of the gene harbors overlapping binding sites for transcription factors Sp1, Sp3, and NF-1 but no homologs of previously characterized IFN-gamma response elements.
T12 1746-1877 DRI_Outcome denotes The putative IFN-gamma response region was sufficient to confer inhibition of reporter gene expression by treatment with IFN-gamma.
T13 1878-2087 DRI_Background denotes Gel mobility shift analysis showed that two distinct and specific DNA-protein complexes were formed when fibroblast nuclear extracts were incubated with oligonucleotides spanning the IFN-gamma response region.
T14 2088-2168 DRI_Approach denotes IFN-gamma did not modify the ability of nuclear proteins to bind to this region.
T15 2169-2294 DRI_Outcome denotes The results indicate that IFN-gamma inhibits COL1A1 expression in fibroblasts principally at the level of gene transcription.
T16 2295-2432 DRI_Challenge denotes Inhibition involves IRF-1 and is mediated through a short proximal promoter segment but without an apparent change in promoter occupancy.
T17 2433-2534 DRI_Background denotes The findings provide novel insight into the mechanism of IFN-gamma regulation of fibroblast function.