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PubMed:10077593 JSONTXT

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PMID_GLOBAL

Id Subject Object Predicate Lexical cue
T1 0-73 Sentence denotes Angiostatin binds ATP synthase on the surface of human endothelial cells.
T2 74-230 Sentence denotes Angiostatin, a proteolytic fragment of plasminogen, is a potent antagonist of angiogenesis and an inhibitor of endothelial cell migration and proliferation.
T3 231-509 Sentence denotes To determine whether the mechanism by which angiostatin inhibits endothelial cell migration and/or proliferation involves binding to cell surface plasminogen receptors, we isolated the binding proteins for plasminogen and angiostatin from human umbilical vein endothelial cells.
T4 510-625 Sentence denotes Binding studies demonstrated that plasminogen and angiostatin bound in a concentration-dependent, saturable manner.
T5 626-767 Sentence denotes Plasminogen binding was unaffected by a 100-fold molar excess of angiostatin, indicating the presence of a distinct angiostatin binding site.
T6 768-1005 Sentence denotes This finding was confirmed by ligand blot analysis of isolated human umbilical vein endothelial cell plasma membrane fractions, which demonstrated that plasminogen bound to a 44-kDa protein, whereas angiostatin bound to a 55-kDa species.
T7 1006-1234 Sentence denotes Amino-terminal sequencing coupled with peptide mass fingerprinting and immunologic analyses identified the plasminogen binding protein as annexin II and the angiostatin binding protein as the alpha/beta-subunits of ATP synthase.
T8 1235-1348 Sentence denotes The presence of this protein on the cell surface was confirmed by flow cytometry and immunofluorescence analysis.
T9 1349-1507 Sentence denotes Angiostatin also bound to the recombinant alpha-subunit of human ATP synthase, and this binding was not inhibited by a 2,500-fold molar excess of plasminogen.
T10 1508-1660 Sentence denotes Angiostatin's antiproliferative effect on endothelial cells was inhibited by as much as 90% in the presence of anti-alpha-subunit ATP synthase antibody.
T11 1661-1858 Sentence denotes Binding of angiostatin to the alpha/beta-subunits of ATP synthase on the cell surface may mediate its antiangiogenic effects and the down-regulation of endothelial cell proliferation and migration.