Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-73 |
Sentence |
denotes |
Angiostatin binds ATP synthase on the surface of human endothelial cells. |
T2 |
74-230 |
Sentence |
denotes |
Angiostatin, a proteolytic fragment of plasminogen, is a potent antagonist of angiogenesis and an inhibitor of endothelial cell migration and proliferation. |
T3 |
231-509 |
Sentence |
denotes |
To determine whether the mechanism by which angiostatin inhibits endothelial cell migration and/or proliferation involves binding to cell surface plasminogen receptors, we isolated the binding proteins for plasminogen and angiostatin from human umbilical vein endothelial cells. |
T4 |
510-625 |
Sentence |
denotes |
Binding studies demonstrated that plasminogen and angiostatin bound in a concentration-dependent, saturable manner. |
T5 |
626-767 |
Sentence |
denotes |
Plasminogen binding was unaffected by a 100-fold molar excess of angiostatin, indicating the presence of a distinct angiostatin binding site. |
T6 |
768-1005 |
Sentence |
denotes |
This finding was confirmed by ligand blot analysis of isolated human umbilical vein endothelial cell plasma membrane fractions, which demonstrated that plasminogen bound to a 44-kDa protein, whereas angiostatin bound to a 55-kDa species. |
T7 |
1006-1234 |
Sentence |
denotes |
Amino-terminal sequencing coupled with peptide mass fingerprinting and immunologic analyses identified the plasminogen binding protein as annexin II and the angiostatin binding protein as the alpha/beta-subunits of ATP synthase. |
T8 |
1235-1348 |
Sentence |
denotes |
The presence of this protein on the cell surface was confirmed by flow cytometry and immunofluorescence analysis. |
T9 |
1349-1507 |
Sentence |
denotes |
Angiostatin also bound to the recombinant alpha-subunit of human ATP synthase, and this binding was not inhibited by a 2,500-fold molar excess of plasminogen. |
T10 |
1508-1660 |
Sentence |
denotes |
Angiostatin's antiproliferative effect on endothelial cells was inhibited by as much as 90% in the presence of anti-alpha-subunit ATP synthase antibody. |
T11 |
1661-1858 |
Sentence |
denotes |
Binding of angiostatin to the alpha/beta-subunits of ATP synthase on the cell surface may mediate its antiangiogenic effects and the down-regulation of endothelial cell proliferation and migration. |