| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-72 |
Sentence |
denotes |
Transcriptional activation by artificial recruitment in mammalian cells. |
| T2 |
73-335 |
Sentence |
denotes |
We show that the typical "nonclassical" activator, which comprises a fusion protein bearing a component of the transcriptional machinery fused to a DNA-binding domain, activates transcription in mammalian cells only weakly when tested with an array of promoters. |
| T3 |
336-490 |
Sentence |
denotes |
However, as found in analogous "artificial recruitment" experiments performed in yeast, these activators work synergistically with "classical" activators. |
| T4 |
491-681 |
Sentence |
denotes |
The effect of the classical activator in such experiments requires that it be tethered to DNA, a requirement that cannot be overcome by expression of that classical activator at high levels. |
| T5 |
682-893 |
Sentence |
denotes |
The effect of the one nonclassical activator that does elicit significant levels of transcription when working alone (i.e., that bearing TATA box-binding protein) is strongly influenced by promoter architecture. |
| T6 |
894-991 |
Sentence |
denotes |
The results, consistent with those of analogous experiments in yeast [see the accompanying paper: |
| T7 |
992-1053 |
Sentence |
denotes |
Gaudreau, L., Keaveney, M., Nevado, J., Zaman, Z., Bryant, G. |
| T8 |
1054-1095 |
Sentence |
denotes |
O., Struhl, K. & Ptashne, M. (1999) Proc. |
| T9 |
1096-1101 |
Sentence |
denotes |
Natl. |
| T10 |
1102-1107 |
Sentence |
denotes |
Acad. |
| T11 |
1108-1112 |
Sentence |
denotes |
Sci. |
| T12 |
1113-1307 |
Sentence |
denotes |
USA 96, 2668-2673], suggest that classical activators, presumably by virtue of their abilities to interact with multiple targets, have a functional flexibility that nonclassical activators lack. |
