PubMed:10075873 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T1	13-48	protein	denotes	intracellular phosphorylated STAT-1
T2	123-139	protein	denotes	interferon-gamma
T3	154-169	cell_type	denotes	human monocytes
T4	190-211	protein	denotes	monoclonal antibodies
T5	276-282	protein	denotes	STAT-1
T6	332-338	protein	denotes	STAT-1
T7	554-570	protein	denotes	interferon-gamma
T8	740-768	protein	denotes	activation modified proteins

Id	Subject	Object	Predicate	Lexical cue
C1	52-66	NP	denotes	flow cytometry
C2	84-98	NP	denotes	flow cytometry
C3	171-184	NP	denotes	This approach
C4	190-211	NP	denotes	monoclonal antibodies
C5	212-216	NP	denotes	that
C6	284-286	NP	denotes	It
C7	465-467	NP	denotes	it
C8	515-520	NP	denotes	cells
C9	521-525	NP	denotes	that
C10	536-541	NP	denotes	their
C11	572-585	NP	denotes	This approach
C12	687-706	NP	denotes	monoclonal reagents
C13	707-711	NP	denotes	that
C14	924-938	NP	denotes	this technique
R1	C2	C1	coref-ident	flow cytometry,flow cytometry
R2	C3	C2	coref-ident	This approach,flow cytometry
R3	C5	C4	coref-relat	that,monoclonal antibodies
R4	C6	C3	coref-pron	It,This approach
R5	C7	C3	coref-pron	it,This approach
R6	C9	C8	coref-relat	that,cells
R7	C10	C8	coref-pron	their,cells
R8	C11	C3	coref-ident	This approach,This approach
R9	C13	C12	coref-relat	that,monoclonal reagents
R10	C14	C11	coref-ident	this technique,This approach

Id	Subject	Object	Predicate	Lexical cue
S1	0-67	Sentence	denotes	Detection of intracellular phosphorylated STAT-1 by flow cytometry.
S2	68-170	Sentence	denotes	We have applied flow cytometry to the investigation of interferon-gamma activation of human monocytes.
S3	171-283	Sentence	denotes	This approach uses monoclonal antibodies that distinguish between the native and phosphorylated forms of STAT-1.
S4	284-451	Sentence	denotes	It enables rapid and quantitative assessment of STAT-1 phosphorylation on a discrete cell basis and is both more sensitive and less time consuming than immunoblotting.
S5	452-571	Sentence	denotes	Furthermore, it allows for discrimination between a mixture of cells that differ in their response to interferon-gamma.
S6	572-769	Sentence	denotes	This approach should allow for the evaluation of different intracellular signaling pathways using a combination of monoclonal reagents that are specific for native and activation modified proteins.
S7	770-910	Sentence	denotes	Application of this form of testing should prove valuable in screening for signaling defects in selected patients with recurrent infections.
S8	911-1032	Sentence	denotes	In addition, this technique should permit dissection of a full range of cellular signaling pathways at the protein level.

Id	Subject	Object	Predicate	Lexical cue
T1	13-48	protein_molecule	denotes	intracellular phosphorylated STAT-1
T2	52-66	other_name	denotes	flow cytometry
T3	84-98	other_name	denotes	flow cytometry
T4	123-139	protein_molecule	denotes	interferon-gamma
T5	154-169	cell_type	denotes	human monocytes
T6	190-211	protein_family_or_group	denotes	monoclonal antibodies
T7	276-282	protein_molecule	denotes	STAT-1
T8	332-338	protein_molecule	denotes	STAT-1
T9	360-379	other_name	denotes	discrete cell basis
T10	436-450	other_name	denotes	immunoblotting
T11	554-570	protein_molecule	denotes	interferon-gamma
T12	631-644	other_name	denotes	intracellular
T13	645-663	other_name	denotes	signaling pathways
T14	687-706	other_organic_compound	denotes	monoclonal reagents
T15	740-768	protein_family_or_group	denotes	activation modified proteins
T16	845-862	other_name	denotes	signaling defects
T17	866-883	multi_cell	denotes	selected patients
T18	983-991	other_name	denotes	cellular
T19	992-1010	other_name	denotes	signaling pathways
T20	1018-1031	other_name	denotes	protein level

Id	Subject	Object	Predicate	Lexical cue
T1	889-909	HP_0002719	denotes	recurrent infections