| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-157 |
Sentence |
denotes |
Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16S rRNA gene fingerprints and community-level physiological profiles. |
| T2 |
158-338 |
Sentence |
denotes |
The effect of three phenyl urea herbicides (diuron, linuron, and chlorotoluron) on soil microbial communities was studied by using soil samples with a 10-year history of treatment. |
| T3 |
339-441 |
Sentence |
denotes |
Denaturing gradient gel electrophoresis (DGGE) was used for the analysis of 16S rRNA genes (16S rDNA). |
| T4 |
442-579 |
Sentence |
denotes |
The degree of similarity between the 16S rDNA profiles of the communities was quantified by numerically analysing the DGGE band patterns. |
| T5 |
580-725 |
Sentence |
denotes |
Similarity dendrograms showed that the microbial community structures of the herbicide-treated and nontreated soils were significantly different. |
| T6 |
726-998 |
Sentence |
denotes |
Moreover, the bacterial diversity seemed to decrease in soils treated with urea herbicides, and sequence determination of several DGGE fragments showed that the most affected species in the soils treated with diuron and linuron belonged to an uncultivated bacterial group. |
| T7 |
999-1115 |
Sentence |
denotes |
As well as the 16S rDNA fingerprints, the substrate utilization patterns of the microbial communities were compared. |
| T8 |
1116-1291 |
Sentence |
denotes |
Principal-component analysis performed on BIOLOG data showed that the functional abilities of the soil microbial communities were altered by the application of the herbicides. |
| T9 |
1292-1614 |
Sentence |
denotes |
In addition, enrichment cultures of the different soils in medium with the urea herbicides as the sole carbon and nitrogen source showed that there was no difference between treated and nontreated soil in the rate of transformation of diuron and chlorotoluron but that there was a strong difference in the case of linuron. |
| T10 |
1615-1827 |
Sentence |
denotes |
In the enrichment cultures with linuron-treated soil, linuron disappeared completely after 1 week whereas no significant transformation was observed in cultures inoculated with nontreated soil even after 4 weeks. |
| T11 |
1828-2007 |
Sentence |
denotes |
In conclusion, this study showed that both the structure and metabolic potential of soil microbial communities were clearly affected by a long-term application of urea herbicides. |
