PubMed:10037751 JSON TXT

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sentences

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":91},"obj":"Sentence"},{"id":"T2","span":{"begin":92,"end":222},"obj":"Sentence"},{"id":"T3","span":{"begin":223,"end":375},"obj":"Sentence"},{"id":"T4","span":{"begin":376,"end":600},"obj":"Sentence"},{"id":"T5","span":{"begin":601,"end":822},"obj":"Sentence"},{"id":"T6","span":{"begin":823,"end":949},"obj":"Sentence"},{"id":"T7","span":{"begin":950,"end":1176},"obj":"Sentence"},{"id":"T8","span":{"begin":1177,"end":1287},"obj":"Sentence"},{"id":"T9","span":{"begin":1288,"end":1518},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

jnlpba-st-training

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":25,"end":42},"obj":"DNA"},{"id":"T2","span":{"begin":61,"end":90},"obj":"DNA"},{"id":"T3","span":{"begin":96,"end":123},"obj":"protein"},{"id":"T4","span":{"begin":159,"end":173},"obj":"cell_line"},{"id":"T5","span":{"begin":178,"end":195},"obj":"DNA"},{"id":"T6","span":{"begin":210,"end":221},"obj":"cell_type"},{"id":"T7","span":{"begin":242,"end":277},"obj":"DNA"},{"id":"T8","span":{"begin":285,"end":302},"obj":"DNA"},{"id":"T9","span":{"begin":306,"end":313},"obj":"cell_type"},{"id":"T10","span":{"begin":318,"end":329},"obj":"cell_type"},{"id":"T11","span":{"begin":376,"end":384},"obj":"DNA"},{"id":"T12","span":{"begin":406,"end":437},"obj":"DNA"},{"id":"T13","span":{"begin":453,"end":494},"obj":"cell_type"},{"id":"T14","span":{"begin":504,"end":513},"obj":"DNA"},{"id":"T15","span":{"begin":535,"end":561},"obj":"DNA"},{"id":"T16","span":{"begin":592,"end":599},"obj":"cell_type"},{"id":"T17","span":{"begin":613,"end":633},"obj":"DNA"},{"id":"T18","span":{"begin":639,"end":671},"obj":"DNA"},{"id":"T19","span":{"begin":695,"end":707},"obj":"DNA"},{"id":"T20","span":{"begin":715,"end":726},"obj":"DNA"},{"id":"T21","span":{"begin":741,"end":770},"obj":"DNA"},{"id":"T22","span":{"begin":783,"end":821},"obj":"DNA"},{"id":"T23","span":{"begin":827,"end":839},"obj":"DNA"},{"id":"T24","span":{"begin":954,"end":981},"obj":"DNA"},{"id":"T25","span":{"begin":1021,"end":1036},"obj":"DNA"},{"id":"T26","span":{"begin":1086,"end":1108},"obj":"DNA"},{"id":"T27","span":{"begin":1136,"end":1151},"obj":"DNA"},{"id":"T28","span":{"begin":1164,"end":1175},"obj":"cell_type"},{"id":"T29","span":{"begin":1212,"end":1227},"obj":"DNA"},{"id":"T30","span":{"begin":1292,"end":1307},"obj":"DNA"},{"id":"T31","span":{"begin":1319,"end":1331},"obj":"protein"},{"id":"T32","span":{"begin":1337,"end":1357},"obj":"protein"},{"id":"T33","span":{"begin":1358,"end":1361},"obj":"protein"},{"id":"T34","span":{"begin":1370,"end":1401},"obj":"protein"},{"id":"T35","span":{"begin":1402,"end":1405},"obj":"protein"},{"id":"T36","span":{"begin":1410,"end":1413},"obj":"protein"},{"id":"T37","span":{"begin":1456,"end":1459},"obj":"protein"},{"id":"T38","span":{"begin":1464,"end":1480},"obj":"protein"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

genia-medco-coref

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":21,"end":42},"obj":"NP"},{"id":"C3","span":{"begin":281,"end":302},"obj":"NP"},{"id":"C4","span":{"begin":306,"end":313},"obj":"NP"},{"id":"C5","span":{"begin":318,"end":329},"obj":"NP"},{"id":"C2","span":{"begin":238,"end":329},"obj":"NP"},{"id":"C6","span":{"begin":342,"end":347},"obj":"NP"},{"id":"C7","span":{"begin":402,"end":437},"obj":"NP"},{"id":"C8","span":{"begin":531,"end":561},"obj":"NP"},{"id":"C9","span":{"begin":592,"end":599},"obj":"NP"},{"id":"C11","span":{"begin":711,"end":726},"obj":"NP"},{"id":"C10","span":{"begin":691,"end":726},"obj":"NP"},{"id":"C12","span":{"begin":779,"end":821},"obj":"NP"},{"id":"C13","span":{"begin":823,"end":839},"obj":"NP"},{"id":"C15","span":{"begin":1084,"end":1108},"obj":"NP"},{"id":"C16","span":{"begin":1109,"end":1113},"obj":"NP"},{"id":"C17","span":{"begin":1164,"end":1175},"obj":"NP"},{"id":"C14","span":{"begin":1084,"end":1175},"obj":"NP"},{"id":"C18","span":{"begin":1231,"end":1243},"obj":"NP"},{"id":"C19","span":{"begin":1333,"end":1361},"obj":"NP"},{"id":"C20","span":{"begin":1456,"end":1459},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C1"},{"id":"R2","pred":"coref-pron","subj":"C6","obj":"C2"},{"id":"R3","pred":"coref-ident","subj":"C8","obj":"C7"},{"id":"R4","pred":"coref-ident","subj":"C9","obj":"C4"},{"id":"R5","pred":"coref-ident","subj":"C11","obj":"C3"},{"id":"R6","pred":"coref-ident","subj":"C12","obj":"C8"},{"id":"R7","pred":"coref-ident","subj":"C13","obj":"C10"},{"id":"R8","pred":"coref-relat","subj":"C16","obj":"C15"},{"id":"R9","pred":"coref-ident","subj":"C17","obj":"C5"},{"id":"R10","pred":"coref-ident","subj":"C18","obj":"C14"},{"id":"R11","pred":"coref-ident","subj":"C20","obj":"C19"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

pubmed-sentences-benchmark

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":91},"obj":"Sentence"},{"id":"S2","span":{"begin":92,"end":222},"obj":"Sentence"},{"id":"S3","span":{"begin":223,"end":375},"obj":"Sentence"},{"id":"S4","span":{"begin":376,"end":600},"obj":"Sentence"},{"id":"S5","span":{"begin":601,"end":822},"obj":"Sentence"},{"id":"S6","span":{"begin":823,"end":949},"obj":"Sentence"},{"id":"S7","span":{"begin":950,"end":1176},"obj":"Sentence"},{"id":"S8","span":{"begin":1177,"end":1287},"obj":"Sentence"},{"id":"S9","span":{"begin":1288,"end":1518},"obj":"Sentence"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

GENIAcorpus

GlyCosmos15-HP

{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":250,"end":264},"obj":"Phenotype"},{"id":"T2","span":{"begin":266,"end":268},"obj":"Phenotype"},{"id":"T3","span":{"begin":376,"end":378},"obj":"Phenotype"},{"id":"T4","span":{"begin":504,"end":506},"obj":"Phenotype"},{"id":"T5","span":{"begin":613,"end":627},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0041092"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0041092"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0041092"},{"id":"A4","pred":"hp_id","subj":"T4","obj":"HP:0041092"},{"id":"A5","pred":"hp_id","subj":"T5","obj":"HP:0041092"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

mondo_disease

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":250,"end":264},"obj":"Disease"},{"id":"T2","span":{"begin":266,"end":268},"obj":"Disease"},{"id":"T3","span":{"begin":376,"end":378},"obj":"Disease"},{"id":"T4","span":{"begin":504,"end":506},"obj":"Disease"},{"id":"T5","span":{"begin":613,"end":627},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0000605"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0000605"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0000605"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0000605"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0000605"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

NCBITAXON

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":25,"end":30},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":285,"end":290},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"Body_part"},{"id":"T2","span":{"begin":159,"end":165},"obj":"Body_part"},{"id":"T3","span":{"begin":475,"end":494},"obj":"Body_part"},{"id":"T4","span":{"begin":995,"end":1001},"obj":"Body_part"},{"id":"T5","span":{"begin":1376,"end":1381},"obj":"Body_part"},{"id":"T6","span":{"begin":1387,"end":1392},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000988"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}

Glycosmos15-CL

{"project":"Glycosmos15-CL","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"Cell"},{"id":"T2","span":{"begin":159,"end":165},"obj":"Cell"},{"id":"T3","span":{"begin":214,"end":221},"obj":"Cell"},{"id":"T4","span":{"begin":306,"end":313},"obj":"Cell"},{"id":"T5","span":{"begin":322,"end":329},"obj":"Cell"},{"id":"T6","span":{"begin":475,"end":494},"obj":"Cell"},{"id":"T7","span":{"begin":592,"end":599},"obj":"Cell"},{"id":"T8","span":{"begin":995,"end":1001},"obj":"Cell"},{"id":"T9","span":{"begin":1168,"end":1175},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A6","pred":"cl_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL:0000988"},{"id":"A7","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A8","pred":"cl_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000084"}],"text":"T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer.\nThe GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity."}