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sentences

Id Subject Object Predicate Lexical cue
T1 0-93 Sentence denotes Purification and identification of a novel subunit of protein serine/threonine phosphatase 4.
T2 94-265 Sentence denotes The catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) has greater than 65% amino acid identity to the catalytic subunit of protein phosphatase 2A (PP2AC).
T3 266-363 Sentence denotes Despite this high homology, PP4 does not appear to associate with known PP2A regulatory subunits.
T4 364-532 Sentence denotes As a first step toward characterization of PP4 holoenzymes and identification of putative PP4 regulatory subunits, PP4 was purified from bovine testis soluble extracts.
T5 533-650 Sentence denotes PP4 existed in two complexes of approximately 270-300 and 400-450 kDa as determined by gel filtration chromatography.
T6 651-788 Sentence denotes The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtration chromatographies.
T7 789-962 Sentence denotes The final product contained two major proteins: the PP4 catalytic subunit plus a protein that migrated as a doublet of 120-125 kDa on SDS-polyacrylamide gel electrophoresis.
T8 963-1046 Sentence denotes The associated protein, termed PP4R1, and PP4C also bound to microcystin-Sepharose.
T9 1047-1157 Sentence denotes Mass spectrometry analysis of the purified complex revealed two major peaks, at 35 (PP4C) and 105 kDa (PP4R1).
T10 1158-1286 Sentence denotes Amino acid sequence information of several peptides derived from the 105 kDa protein was utilized to isolate a human cDNA clone.
T11 1287-1424 Sentence denotes Analysis of the predicted amino acid sequence revealed 13 nonidentical repeats similar to repeats found in the A subunit of PP2A (PP2AA).
T12 1425-1567 Sentence denotes The PP4R1 cDNA clone engineered with an N-terminal Myc tag was expressed in COS M6 cells and PP4C co-immunoprecipitated with Myc-tagged PP4R1.
T13 1568-1729 Sentence denotes These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 508-514 http://purl.obolibrary.org/obo/MAT_0000132 denotes testis

bionlp-st-gro-2013-training

Id Subject Object Predicate Lexical cue
T35 1275-1279 DNA denotes cDNA
T36 1313-1323 AminoAcid denotes amino acid
T38 1411-1415 Protein denotes PP2A
T39 1417-1422 ProteinSubunit denotes PP2AA
T40 1429-1434 ProteinSubunit denotes PP4R1
T41 1435-1439 DNA denotes cDNA
T44 1501-1513 Cell denotes COS M6 cells
T45 1518-1522 Protein denotes PP4C
T47 1561-1566 Protein denotes PP4R1
T48 1605-1608 Enzyme denotes PP4
T49 1627-1639 ProteinComplex denotes core complex
T50 1643-1647 Enzyme denotes PP2A
T53 552-561 ProteinComplex denotes complexes
T37 1398-1407 ProteinSubunit denotes A subunit
T1 43-50 ProteinSubunit denotes subunit
T2 54-92 Enzyme denotes protein serine/threonine phosphatase 4
T3 98-115 ProteinSubunit denotes catalytic subunit
T4 119-157 Enzyme denotes protein serine/threonine phosphatase 4
T5 159-163 ProteinSubunit denotes PP4C
T6 186-196 AminoAcid denotes amino acid
T7 213-230 ProteinSubunit denotes catalytic subunit
T8 234-256 Enzyme denotes protein phosphatase 2A
T9 258-263 ProteinSubunit denotes PP2AC
T10 294-297 Enzyme denotes PP4
T11 338-342 Enzyme denotes PP2A
T12 343-362 ProteinSubunit denotes regulatory subunits
T13 407-422 Holoenzyme denotes PP4 holoenzymes
T14 445-457 Enzyme denotes putative PP4
T15 458-477 ProteinSubunit denotes regulatory subunits
T16 479-482 Enzyme denotes PP4
T17 501-507 Eukaryote denotes bovine
T18 508-514 Tissue denotes testis
T19 533-536 Enzyme denotes PP4
T20 663-674 Enzyme denotes PP4 complex
T21 827-835 Protein denotes proteins
T22 841-844 Enzyme denotes PP4
T23 845-862 ProteinSubunit denotes catalytic subunit
T24 870-877 Protein denotes protein
T25 967-985 Protein denotes associated protein
T26 994-999 ProteinSubunit denotes PP4R1
T27 1005-1009 ProteinSubunit denotes PP4C
T28 1090-1097 ProteinComplex denotes complex
T29 1131-1135 ProteinSubunit denotes PP4C
T30 1150-1155 ProteinSubunit denotes PP4R1
T31 1158-1168 AminoAcid denotes Amino acid
T32 1201-1209 Peptide denotes peptides
T33 1235-1242 Protein denotes protein
T34 1269-1274 Eukaryote denotes human
T42 1465-1483 BindingAssay denotes N-terminal Myc tag
T46 1523-1544 BindingAssay denotes co-immunoprecipitated
T54 1673-1690 ProteinSubunit denotes catalytic subunit
T51 1697-1728 ProteinSubunit denotes "PP2AA-like" structural subunit
E1 1488-1497 GeneExpression denotes expressed
R1 T2 T1 hasPart protein serine/threonine phosphatase 4,subunit
R2 T4 T3 hasPart protein serine/threonine phosphatase 4,catalytic subunit
R3 T8 T7 hasPart protein phosphatase 2A,catalytic subunit
R4 T11 T12 hasPart PP2A,regulatory subunits
R5 T14 T15 hasPart putative PP4,regulatory subunits
R6 T18 T17 fromSpecies testis,bovine
R7 T22 T23 hasPart PP4,catalytic subunit
R8 T33 T32 hasPart protein,peptides
R9 T35 T34 fromSpecies cDNA,human
R11 T41 T40 encodes cDNA,PP4R1
R12 E1 T44 locatedIn expressed,COS M6 cells
R14 T53 T19 hasPart complexes,PP4
R15 T28 T29 hasPart complex,PP4C
R16 T28 T30 hasPart complex,PP4R1
R10 T38 T37 hasPart PP2A,A subunit
R12 T41 E1 hasPatient cDNA,expressed

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 508-514 Body_part denotes testis http://purl.obolibrary.org/obo/MAT_0000132

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 501-507 OrganismTaxon denotes bovine 9913
T2 1269-1274 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 508-514 Body_part denotes testis http://purl.obolibrary.org/obo/UBERON_0000473