PubMed:10024668 JSONTXT

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":991,"end":1004},"obj":"Cell"},{"id":"T2","span":{"begin":1038,"end":1051},"obj":"Cell"},{"id":"T3","span":{"begin":1166,"end":1170},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000836"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0017005"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000560"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":66,"end":85},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T2","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T3","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T4","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T5","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T6","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T7","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T8","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T9","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T10","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg128"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":66,"end":85},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T2","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T3","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T4","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T5","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T6","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T7","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T8","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"T9","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"T10","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg129"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":150,"end":372},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":373,"end":503},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":504,"end":670},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":671,"end":830},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":831,"end":906},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":907,"end":980},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":981,"end":1101},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1102,"end":1212},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"T2","span":{"begin":150,"end":372},"obj":"Sentence"},{"id":"T3","span":{"begin":373,"end":503},"obj":"Sentence"},{"id":"T4","span":{"begin":504,"end":670},"obj":"Sentence"},{"id":"T5","span":{"begin":671,"end":830},"obj":"Sentence"},{"id":"T6","span":{"begin":831,"end":906},"obj":"Sentence"},{"id":"T7","span":{"begin":907,"end":980},"obj":"Sentence"},{"id":"T8","span":{"begin":981,"end":1101},"obj":"Sentence"},{"id":"T9","span":{"begin":1102,"end":1212},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"T2","span":{"begin":150,"end":372},"obj":"Sentence"},{"id":"T3","span":{"begin":373,"end":503},"obj":"Sentence"},{"id":"T4","span":{"begin":504,"end":670},"obj":"Sentence"},{"id":"T5","span":{"begin":671,"end":830},"obj":"Sentence"},{"id":"T6","span":{"begin":831,"end":906},"obj":"Sentence"},{"id":"T7","span":{"begin":907,"end":980},"obj":"Sentence"},{"id":"T8","span":{"begin":981,"end":1101},"obj":"Sentence"},{"id":"T9","span":{"begin":1102,"end":1212},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":66,"end":85},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":285,"end":290},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":0,"end":6},"obj":"FMAID:256050"},{"id":"_T2","span":{"begin":31,"end":42},"obj":"FMAID:84055"},{"id":"_T3","span":{"begin":31,"end":42},"obj":"FMAID:84056"},{"id":"_T4","span":{"begin":31,"end":42},"obj":"FMAID:197979"},{"id":"_T5","span":{"begin":31,"end":42},"obj":"FMAID:197978"},{"id":"_T6","span":{"begin":31,"end":42},"obj":"FMAID:226764"},{"id":"_T7","span":{"begin":31,"end":42},"obj":"FMAID:165175"},{"id":"_T8","span":{"begin":31,"end":42},"obj":"FMAID:67093"},{"id":"_T9","span":{"begin":43,"end":50},"obj":"FMAID:90078"},{"id":"_T10","span":{"begin":66,"end":85},"obj":"FMAID:82787"},{"id":"_T11","span":{"begin":66,"end":85},"obj":"FMAID:196781"},{"id":"_T12","span":{"begin":172,"end":192},"obj":"FMAID:82787"},{"id":"_T13","span":{"begin":172,"end":192},"obj":"FMAID:196781"},{"id":"_T14","span":{"begin":285,"end":290},"obj":"FMAID:7197"},{"id":"_T15","span":{"begin":285,"end":290},"obj":"FMAID:93672"},{"id":"_T16","span":{"begin":337,"end":350},"obj":"FMAID:198074"},{"id":"_T17","span":{"begin":423,"end":439},"obj":"FMAID:89997"},{"id":"_T18","span":{"begin":423,"end":439},"obj":"FMAID:198528"},{"id":"_T19","span":{"begin":423,"end":442},"obj":"FMAID:165365"},{"id":"_T20","span":{"begin":423,"end":442},"obj":"FMAID:67572"},{"id":"_T21","span":{"begin":423,"end":442},"obj":"FMAID:67518"},{"id":"_T22","span":{"begin":423,"end":442},"obj":"FMAID:85554"},{"id":"_T23","span":{"begin":423,"end":442},"obj":"FMAID:199979"},{"id":"_T24","span":{"begin":423,"end":442},"obj":"FMAID:165395"},{"id":"_T25","span":{"begin":432,"end":439},"obj":"FMAID:165447"},{"id":"_T26","span":{"begin":432,"end":439},"obj":"FMAID:67257"},{"id":"_T27","span":{"begin":447,"end":458},"obj":"FMAID:196728"},{"id":"_T28","span":{"begin":447,"end":458},"obj":"FMAID:82739"},{"id":"_T29","span":{"begin":551,"end":556},"obj":"FMAID:169002"},{"id":"_T30","span":{"begin":551,"end":556},"obj":"FMAID:68646"},{"id":"_T31","span":{"begin":704,"end":711},"obj":"FMAID:256050"},{"id":"_T32","span":{"begin":850,"end":855},"obj":"FMAID:199098"},{"id":"_T33","span":{"begin":898,"end":905},"obj":"FMAID:256050"},{"id":"_T34","span":{"begin":946,"end":953},"obj":"FMAID:256050"},{"id":"_T35","span":{"begin":974,"end":979},"obj":"FMAID:68646"},{"id":"_T36","span":{"begin":974,"end":979},"obj":"FMAID:169002"},{"id":"_T37","span":{"begin":991,"end":1004},"obj":"FMAID:198513"},{"id":"_T38","span":{"begin":991,"end":1004},"obj":"FMAID:83530"},{"id":"_T39","span":{"begin":991,"end":1004},"obj":"FMAID:197821"},{"id":"_T40","span":{"begin":991,"end":1004},"obj":"FMAID:84375"},{"id":"_T41","span":{"begin":1038,"end":1051},"obj":"FMAID:83032"},{"id":"_T42","span":{"begin":1038,"end":1051},"obj":"FMAID:197292"},{"id":"_T43","span":{"begin":1038,"end":1051},"obj":"FMAID:83031"},{"id":"_T44","span":{"begin":1038,"end":1051},"obj":"FMAID:197290"},{"id":"_T45","span":{"begin":1038,"end":1051},"obj":"FMAID:83030"},{"id":"_T46","span":{"begin":1038,"end":1051},"obj":"FMAID:197293"},{"id":"_T47","span":{"begin":1114,"end":1118},"obj":"FMAID:198663"},{"id":"_T48","span":{"begin":1123,"end":1129},"obj":"FMAID:90078"},{"id":"_T49","span":{"begin":1152,"end":1162},"obj":"FMAID:165175"},{"id":"_T50","span":{"begin":1152,"end":1162},"obj":"FMAID:84055"},{"id":"_T51","span":{"begin":1152,"end":1162},"obj":"FMAID:197978"},{"id":"_T52","span":{"begin":1152,"end":1162},"obj":"FMAID:226764"},{"id":"_T53","span":{"begin":1152,"end":1162},"obj":"FMAID:67093"},{"id":"_T54","span":{"begin":1152,"end":1162},"obj":"FMAID:197979"},{"id":"_T55","span":{"begin":1152,"end":1162},"obj":"FMAID:84056"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":117,"end":148},"obj":"http://www.uniprot.org/uniprot/Q06430"},{"id":"T2","span":{"begin":223,"end":255},"obj":"http://www.uniprot.org/uniprot/Q06430"},{"id":"T3","span":{"begin":117,"end":148},"obj":"http://www.uniprot.org/uniprot/Q8N0V5"},{"id":"T4","span":{"begin":223,"end":255},"obj":"http://www.uniprot.org/uniprot/Q8N0V5"},{"id":"T5","span":{"begin":117,"end":148},"obj":"http://www.uniprot.org/uniprot/Q8NFS9"},{"id":"T6","span":{"begin":223,"end":255},"obj":"http://www.uniprot.org/uniprot/Q8NFS9"},{"id":"T7","span":{"begin":1024,"end":1026},"obj":"http://www.uniprot.org/uniprot/P11150"},{"id":"T8","span":{"begin":1024,"end":1026},"obj":"http://www.uniprot.org/uniprot/P35914"},{"id":"T9","span":{"begin":1024,"end":1026},"obj":"http://www.uniprot.org/uniprot/Q14469"},{"id":"T10","span":{"begin":1071,"end":1075},"obj":"http://www.uniprot.org/uniprot/Q14C87"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":117,"end":148},"obj":"http://www.uniprot.org/uniprot/P97402"},{"id":"T2","span":{"begin":223,"end":255},"obj":"http://www.uniprot.org/uniprot/P97402"},{"id":"T3","span":{"begin":1024,"end":1026},"obj":"http://www.uniprot.org/uniprot/P27656"},{"id":"T4","span":{"begin":1024,"end":1026},"obj":"http://www.uniprot.org/uniprot/P38060"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T2","span":{"begin":551,"end":556},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T3","span":{"begin":704,"end":711},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T4","span":{"begin":898,"end":905},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T5","span":{"begin":946,"end":953},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T6","span":{"begin":974,"end":979},"obj":"http://purl.bioontology.org/ontology/STY/T025"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":573,"end":581},"obj":"http://purl.obolibrary.org/obo/GO_0007349"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":31,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"T2","span":{"begin":1152,"end":1162},"obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"T3","span":{"begin":423,"end":431},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T4","span":{"begin":551,"end":556},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5","span":{"begin":974,"end":979},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6","span":{"begin":1014,"end":1018},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7","span":{"begin":1061,"end":1065},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T2","span":{"begin":704,"end":711},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T3","span":{"begin":898,"end":905},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T4","span":{"begin":946,"end":953},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5","span":{"begin":285,"end":290},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":31,"end":42},"obj":"http://edamontology.org/topic_0624"},{"id":"T2","span":{"begin":31,"end":42},"obj":"http://edamontology.org/topic_0654"},{"id":"T3","span":{"begin":43,"end":50},"obj":"http://edamontology.org/topic_0102"},{"id":"T4","span":{"begin":56,"end":61},"obj":"http://edamontology.org/topic_2815"},{"id":"T5","span":{"begin":152,"end":157},"obj":"http://edamontology.org/topic_2815"},{"id":"T6","span":{"begin":158,"end":162},"obj":"http://edamontology.org/topic_3512"},{"id":"T7","span":{"begin":291,"end":295},"obj":"http://edamontology.org/topic_3512"},{"id":"T8","span":{"begin":312,"end":317},"obj":"http://edamontology.org/topic_0632"},{"id":"T9","span":{"begin":337,"end":341},"obj":"http://edamontology.org/topic_3512"},{"id":"T10","span":{"begin":342,"end":350},"obj":"http://edamontology.org/topic_3168"},{"id":"T11","span":{"begin":342,"end":350},"obj":"http://edamontology.org/topic_0080"},{"id":"T12","span":{"begin":377,"end":381},"obj":"http://edamontology.org/topic_3512"},{"id":"T13","span":{"begin":401,"end":409},"obj":"http://edamontology.org/topic_3168"},{"id":"T14","span":{"begin":401,"end":409},"obj":"http://edamontology.org/topic_0080"},{"id":"T15","span":{"begin":423,"end":439},"obj":"http://edamontology.org/topic_0820"},{"id":"T16","span":{"begin":432,"end":439},"obj":"http://edamontology.org/topic_0078"},{"id":"T17","span":{"begin":447,"end":458},"obj":"http://edamontology.org/topic_0154"},{"id":"T18","span":{"begin":532,"end":537},"obj":"http://edamontology.org/topic_2815"},{"id":"T19","span":{"begin":538,"end":542},"obj":"http://edamontology.org/topic_3512"},{"id":"T20","span":{"begin":631,"end":635},"obj":"http://edamontology.org/topic_3512"},{"id":"T21","span":{"begin":657,"end":662},"obj":"http://edamontology.org/topic_2815"},{"id":"T22","span":{"begin":850,"end":855},"obj":"http://edamontology.org/topic_3512"},{"id":"T23","span":{"begin":1123,"end":1129},"obj":"http://edamontology.org/topic_0102"},{"id":"T24","span":{"begin":1146,"end":1151},"obj":"http://edamontology.org/topic_2815"},{"id":"T25","span":{"begin":1152,"end":1162},"obj":"http://edamontology.org/topic_0624"},{"id":"T26","span":{"begin":1152,"end":1162},"obj":"http://edamontology.org/topic_0654"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":31,"end":50},"obj":"http://edamontology.org/data_1283"},{"id":"T2","span":{"begin":43,"end":50},"obj":"http://edamontology.org/data_1274"},{"id":"T3","span":{"begin":43,"end":50},"obj":"http://edamontology.org/operation_2429"},{"id":"T4","span":{"begin":43,"end":50},"obj":"http://edamontology.org/format_3285"},{"id":"T5","span":{"begin":337,"end":350},"obj":"http://edamontology.org/data_2977"},{"id":"T6","span":{"begin":337,"end":350},"obj":"http://edamontology.org/data_3494"},{"id":"T7","span":{"begin":342,"end":350},"obj":"http://edamontology.org/operation_3218"},{"id":"T8","span":{"begin":342,"end":350},"obj":"http://edamontology.org/data_2044"},{"id":"T9","span":{"begin":401,"end":409},"obj":"http://edamontology.org/operation_3218"},{"id":"T10","span":{"begin":401,"end":409},"obj":"http://edamontology.org/data_2044"},{"id":"T11","span":{"begin":401,"end":419},"obj":"http://edamontology.org/data_1094"},{"id":"T12","span":{"begin":432,"end":439},"obj":"http://edamontology.org/data_1467"},{"id":"T13","span":{"begin":432,"end":439},"obj":"http://edamontology.org/format_1208"},{"id":"T14","span":{"begin":607,"end":620},"obj":"http://edamontology.org/operation_2246"},{"id":"T15","span":{"begin":671,"end":684},"obj":"http://edamontology.org/data_2866"},{"id":"T16","span":{"begin":685,"end":693},"obj":"http://edamontology.org/operation_2945"},{"id":"T17","span":{"begin":697,"end":703},"obj":"http://edamontology.org/operation_3435"},{"id":"T18","span":{"begin":934,"end":942},"obj":"http://edamontology.org/operation_2423"},{"id":"T19","span":{"begin":1114,"end":1129},"obj":"http://edamontology.org/data_1285"},{"id":"T20","span":{"begin":1123,"end":1129},"obj":"http://edamontology.org/operation_2429"},{"id":"T21","span":{"begin":1123,"end":1129},"obj":"http://edamontology.org/data_1274"},{"id":"T22","span":{"begin":1123,"end":1129},"obj":"http://edamontology.org/format_3285"},{"id":"T23","span":{"begin":1137,"end":1142},"obj":"http://edamontology.org/data_2012"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":257,"end":263},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":365,"end":371},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":420,"end":422},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":496,"end":502},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"PD-GlycoGenes20190927-B_T8","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"PD-GlycoGenes20190927-B_T9","span":{"begin":582,"end":588},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"PD-GlycoGenes20190927-B_T10","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg129"},{"id":"PD-GlycoGenes20190927-B_T11","span":{"begin":663,"end":669},"obj":"https://acgg.asia/db/ggdb/info/gg128"},{"id":"PD-GlycoGenes20190927-B_T12","span":{"begin":907,"end":912},"obj":"https://acgg.asia/db/ggdb/info/gg171"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"10024668-7#90#94#gene121256","span":{"begin":1071,"end":1075},"obj":"gene121256"},{"id":"10024668-7#10#32#diseaseC0023487","span":{"begin":991,"end":1013},"obj":"diseaseC0023487"},{"id":"10024668-7#10#32#diseaseC2745900","span":{"begin":991,"end":1013},"obj":"diseaseC2745900"},{"id":"10024668-7#57#79#diseaseC0023448","span":{"begin":1038,"end":1060},"obj":"diseaseC0023448"},{"id":"10024668-7#57#79#diseaseC0023449","span":{"begin":1038,"end":1060},"obj":"diseaseC0023449"},{"id":"10024668-7#57#79#diseaseC1961102","span":{"begin":1038,"end":1060},"obj":"diseaseC1961102"}],"relations":[{"id":"90#94#gene12125610#32#diseaseC0023487","pred":"associated_with","subj":"10024668-7#90#94#gene121256","obj":"10024668-7#10#32#diseaseC0023487"},{"id":"90#94#gene12125610#32#diseaseC2745900","pred":"associated_with","subj":"10024668-7#90#94#gene121256","obj":"10024668-7#10#32#diseaseC2745900"},{"id":"90#94#gene12125657#79#diseaseC0023448","pred":"associated_with","subj":"10024668-7#90#94#gene121256","obj":"10024668-7#57#79#diseaseC0023448"},{"id":"90#94#gene12125657#79#diseaseC0023449","pred":"associated_with","subj":"10024668-7#90#94#gene121256","obj":"10024668-7#57#79#diseaseC0023449"},{"id":"90#94#gene12125657#79#diseaseC1961102","pred":"associated_with","subj":"10024668-7#90#94#gene121256","obj":"10024668-7#57#79#diseaseC1961102"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":285,"end":290},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":285,"end":290},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":0,"end":6},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":704,"end":711},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":898,"end":905},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":946,"end":953},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"bionlp-st-gro-2013-development","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"Tissue"},{"id":"T3","span":{"begin":31,"end":42},"obj":"Chromosome"},{"id":"T4","span":{"begin":56,"end":61},"obj":"Eukaryote"},{"id":"T5","span":{"begin":62,"end":148},"obj":"Enzyme"},{"id":"T6","span":{"begin":152,"end":157},"obj":"Eukaryote"},{"id":"T7","span":{"begin":158,"end":162},"obj":"DNA"},{"id":"T9","span":{"begin":257,"end":263},"obj":"Enzyme"},{"id":"T10","span":{"begin":285,"end":290},"obj":"Tissue"},{"id":"T11","span":{"begin":291,"end":303},"obj":"DNA"},{"id":"T12","span":{"begin":337,"end":350},"obj":"MessengerRNA"},{"id":"T13","span":{"begin":358,"end":364},"obj":"Eukaryote"},{"id":"T14","span":{"begin":365,"end":371},"obj":"Enzyme"},{"id":"T15","span":{"begin":377,"end":381},"obj":"DNA"},{"id":"T16","span":{"begin":415,"end":439},"obj":"Protein"},{"id":"T17","span":{"begin":447,"end":458},"obj":"AminoAcid"},{"id":"T18","span":{"begin":489,"end":495},"obj":"Eukaryote"},{"id":"T19","span":{"begin":496,"end":502},"obj":"Enzyme"},{"id":"T21","span":{"begin":532,"end":537},"obj":"Eukaryote"},{"id":"T22","span":{"begin":538,"end":542},"obj":"DNA"},{"id":"T23","span":{"begin":546,"end":556},"obj":"Cell"},{"id":"T24","span":{"begin":582,"end":588},"obj":"Enzyme"},{"id":"T25","span":{"begin":631,"end":635},"obj":"DNA"},{"id":"T26","span":{"begin":657,"end":662},"obj":"Eukaryote"},{"id":"T27","span":{"begin":663,"end":669},"obj":"Enzyme"},{"id":"T28","span":{"begin":704,"end":711},"obj":"Tissue"},{"id":"T29","span":{"begin":760,"end":764},"obj":"MessengerRNA"},{"id":"T30","span":{"begin":798,"end":807},"obj":"Enzyme"},{"id":"T32","span":{"begin":850,"end":855},"obj":"MessengerRNA"},{"id":"T34","span":{"begin":898,"end":905},"obj":"Tissue"},{"id":"T35","span":{"begin":924,"end":928},"obj":"MessengerRNA"},{"id":"T36","span":{"begin":946,"end":953},"obj":"Tissue"},{"id":"T37","span":{"begin":964,"end":979},"obj":"Cell"},{"id":"T39","span":{"begin":1024,"end":1029},"obj":"Cell"},{"id":"T40","span":{"begin":1038,"end":1070},"obj":"Cell"},{"id":"T41","span":{"begin":1071,"end":1077},"obj":"Cell"},{"id":"T42","span":{"begin":1096,"end":1100},"obj":"MessengerRNA"},{"id":"T43","span":{"begin":1114,"end":1118},"obj":"Gene"},{"id":"T44","span":{"begin":1146,"end":1151},"obj":"Eukaryote"},{"id":"T45","span":{"begin":1152,"end":1164},"obj":"Chromosome"},{"id":"T46","span":{"begin":1178,"end":1211},"obj":"ExperimentalMethod"},{"id":"E1","span":{"begin":16,"end":26},"obj":"GeneExpression"},{"id":"E2","span":{"begin":504,"end":524},"obj":"GeneExpression"},{"id":"E3","span":{"begin":812,"end":821},"obj":"TranscriptionOfGene"},{"id":"E4","span":{"begin":860,"end":869},"obj":"TranscriptionOfGene"}],"relations":[{"id":"R1","pred":"locatedIn","subj":"E1","obj":"T1"},{"id":"R2","pred":"fromSpecies","subj":"T5","obj":"T4"},{"id":"R3","pred":"encodes","subj":"T7","obj":"T9"},{"id":"R4","pred":"fromSpecies","subj":"T7","obj":"T6"},{"id":"R5","pred":"locatedIn","subj":"T11","obj":"T10"},{"id":"R6","pred":"encodes","subj":"T12","obj":"T14"},{"id":"R7","pred":"fromSpecies","subj":"T14","obj":"T13"},{"id":"R8","pred":"encodes","subj":"T15","obj":"T16"},{"id":"R9","pred":"fromSpecies","subj":"T19","obj":"T18"},{"id":"R10","pred":"locatedIn","subj":"E2","obj":"T23"},{"id":"R11","pred":"fromSpecies","subj":"T22","obj":"T21"},{"id":"R12","pred":"encodes","subj":"T25","obj":"T27"},{"id":"R13","pred":"fromSpecies","subj":"T27","obj":"T26"},{"id":"R14","pred":"encodes","subj":"T29","obj":"T30"},{"id":"R15","pred":"locatedIn","subj":"T29","obj":"T28"},{"id":"R16","pred":"locatedIn","subj":"E4","obj":"T34"},{"id":"R17","pred":"locatedIn","subj":"T35","obj":"T37"},{"id":"R18","pred":"locatedIn","subj":"T37","obj":"T36"},{"id":"R19","pred":"locatedIn","subj":"T42","obj":"T39"},{"id":"R20","pred":"locatedIn","subj":"T42","obj":"T41"},{"id":"R21","pred":"fromSpecies","subj":"T45","obj":"T44"},{"id":"R22","pred":"locatedIn","subj":"T45","obj":"T43"},{"id":"R23","pred":"hasPart","subj":"T16","obj":"T17"},{"id":"R25","pred":"hasPatient","subj":"T5","obj":"E1"},{"id":"R26","pred":"hasPatient","subj":"T22","obj":"E2"},{"id":"R27","pred":"hasPatient","subj":"T29","obj":"E3"},{"id":"R28","pred":"hasPatient","subj":"T32","obj":"E4"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":1005,"end":1013},"obj":"HP_0001909"},{"id":"T2","span":{"begin":1052,"end":1060},"obj":"HP_0001909"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":1071,"end":1075},"obj":"gene:121256"},{"id":"T1","span":{"begin":1038,"end":1060},"obj":"disease:C0023448"},{"id":"T2","span":{"begin":1071,"end":1075},"obj":"gene:121256"},{"id":"T3","span":{"begin":1038,"end":1060},"obj":"disease:C0023449"},{"id":"T4","span":{"begin":1071,"end":1075},"obj":"gene:121256"},{"id":"T5","span":{"begin":1038,"end":1060},"obj":"disease:C1961102"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":285,"end":290},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":1005,"end":1013},"obj":"Phenotype"},{"id":"T2","span":{"begin":1052,"end":1060},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0001909"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0001909"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":991,"end":1013},"obj":"Disease"},{"id":"T2","span":{"begin":1038,"end":1060},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0012883"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0001018"},{"id":"A3","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0004967"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":56,"end":61},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":152,"end":157},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":358,"end":364},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":489,"end":495},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":532,"end":537},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":657,"end":662},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":1146,"end":1151},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9913"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9913"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"},{"id":"A7","pred":"db_id","subj":"T7","obj":"9606"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":0,"end":6},"obj":"Body_part"},{"id":"T2","span":{"begin":285,"end":290},"obj":"Body_part"},{"id":"T3","span":{"begin":423,"end":431},"obj":"Body_part"},{"id":"T6","span":{"begin":1152,"end":1162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000094"},{"id":"A5","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/GO_0005694"}],"text":"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.\nA human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization."}