PubMed:10022897
Annnotations
PMID_GLOBAL
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 312-323 | DiseaseOrPhenotypicFeature | denotes | sensitivity | 0000605 |
jnlpba-st-training
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 55-73 | DNA | denotes | human Fas promoter |
| T2 | 83-100 | protein | denotes | NF-kappaB p50-p65 |
| T3 | 114-117 | protein | denotes | Fas |
| T4 | 119-123 | protein | denotes | CD95 |
| T5 | 129-139 | protein | denotes | Fas ligand |
| T6 | 141-146 | protein | denotes | CD95L |
| T7 | 274-277 | protein | denotes | Fas |
| T8 | 327-331 | protein | denotes | FasL |
| T9 | 563-566 | protein | denotes | Fas |
| T10 | 670-688 | cell_line | denotes | Jurkat T-cell line |
| T11 | 775-790 | protein | denotes | T-cell receptor |
| T12 | 812-832 | cell_line | denotes | resting Jurkat cells |
| T13 | 841-844 | protein | denotes | Fas |
| T14 | 846-854 | RNA | denotes | Fas mRNA |
| T15 | 924-951 | DNA | denotes | sequential deletion mutants |
| T16 | 959-977 | DNA | denotes | human fas promoter |
| T17 | 1028-1042 | DNA | denotes | 47-bp sequence |
| T18 | 1044-1066 | DNA | denotes | positions -306 to -260 |
| T19 | 1193-1228 | DNA | denotes | unrecognized composite binding site |
| T20 | 1242-1245 | protein | denotes | Sp1 |
| T21 | 1250-1259 | protein | denotes | NF-kappaB |
| T22 | 1260-1281 | protein | denotes | transcription factors |
| T23 | 1285-1307 | DNA | denotes | positions -295 to -286 |
| T24 | 1427-1430 | protein | denotes | Sp1 |
| T25 | 1488-1518 | protein | denotes | p50-p65 NF-kappaB heterodimers |
| T26 | 1541-1544 | protein | denotes | Sp1 |
| T27 | 1549-1558 | protein | denotes | NF-kappaB |
| T28 | 1678-1681 | protein | denotes | Sp1 |
| T29 | 1698-1701 | protein | denotes | p50 |
| T30 | 1738-1763 | DNA | denotes | kappaB-Sp1 composite site |
| T31 | 1767-1793 | DNA | denotes | P/I-inducible fas promoter |
| T32 | 1834-1837 | protein | denotes | Sp1 |
| T33 | 1851-1863 | DNA | denotes | -295 to -286 |
| T34 | 1870-1921 | DNA | denotes | thymidine kinase promoter-driven reporter construct |
| T35 | 1926-1952 | DNA | denotes | native promoter constructs |
| T36 | 1956-1968 | cell_line | denotes | Jurkat cells |
| T37 | 1984-1997 | protein | denotes | IkappaB-alpha |
| T38 | 2068-2086 | DNA | denotes | kappaB-Sp1 element |
genia-medco-coref
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| C1 | 51-73 | NP | denotes | the human Fas promoter |
| C2 | 114-124 | NP | denotes | Fas (CD95) |
| C3 | 254-288 | NP | denotes | the upregulation of Fas expression |
| C4 | 361-377 | NP | denotes | Fas upregulation |
| C5 | 492-504 | NP | denotes | this process |
| C6 | 544-579 | NP | denotes | activation-induced Fas upregulation |
| C7 | 702-747 | NP | denotes | phorbol myristate acetate and ionomycin (P/I) |
| C8 | 749-801 | NP | denotes | pharmacological mimics of T-cell receptor activation |
| C9 | 841-844 | NP | denotes | Fas |
| C10 | 955-977 | NP | denotes | the human fas promoter |
| C11 | 1101-1135 | NP | denotes | activation-driven fas upregulation |
| C12 | 1285-1307 | NP | denotes | positions -295 to -286 |
| C13 | 1309-1352 | NP | denotes | Electrophoretic mobility shift assay (EMSA) |
| C14 | 1380-1391 | NP | denotes | this region |
| C15 | 1626-1630 | NP | denotes | EMSA |
| C16 | 1734-1763 | NP | denotes | the kappaB-Sp1 composite site |
| C17 | 2120-2129 | NP | denotes | this site |
| R1 | C4 | C3 | coref-ident | Fas upregulation,the upregulation of Fas expression |
| R2 | C5 | C4 | coref-ident | this process,Fas upregulation |
| R3 | C6 | C5 | coref-ident | activation-induced Fas upregulation,this process |
| R4 | C8 | C7 | coref-appos | pharmacological mimics of T-cell receptor activation,phorbol myristate acetate and ionomycin (P/I) |
| R5 | C9 | C2 | coref-ident | Fas,Fas (CD95) |
| R6 | C10 | C1 | coref-ident | the human fas promoter,the human Fas promoter |
| R7 | C11 | C6 | coref-ident | activation-driven fas upregulation,activation-induced Fas upregulation |
| R8 | C14 | C12 | coref-ident | this region,positions -295 to -286 |
| R9 | C15 | C13 | coref-ident | EMSA,Electrophoretic mobility shift assay (EMSA) |
| R10 | C17 | C16 | coref-ident | this site,the kappaB-Sp1 composite site |
pubmed-sentences-benchmark
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| S1 | 0-113 | Sentence | denotes | Activation-dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment. |
| S2 | 114-220 | Sentence | denotes | Fas (CD95) and Fas ligand (CD95L) are an interacting receptor-ligand pair required for immune homeostasis. |
| S3 | 221-351 | Sentence | denotes | Lymphocyte activation results in the upregulation of Fas expression and the acquisition of sensitivity to FasL-mediated apoptosis. |
| S4 | 352-505 | Sentence | denotes | Although Fas upregulation is central to the preservation of immunologic tolerance, little is known about the molecular machinery underlying this process. |
| S5 | 506-802 | Sentence | denotes | To investigate the events involved in activation-induced Fas upregulation, we have examined mRNA accumulation, fas promoter activity, and protein expression in the Jurkat T-cell line treated with phorbol myristate acetate and ionomycin (P/I), pharmacological mimics of T-cell receptor activation. |
| S6 | 803-917 | Sentence | denotes | Although resting Jurkat cells express Fas, Fas mRNA was induced approximately 10-fold in 2 h upon P/I stimulation. |
| S7 | 918-1136 | Sentence | denotes | Using sequential deletion mutants of the human fas promoter in transient transfection assays, we identified a 47-bp sequence (positions -306 to -260 relative to the ATG) required for activation-driven fas upregulation. |
| S8 | 1137-1308 | Sentence | denotes | Sequence analysis revealed the presence of a previously unrecognized composite binding site for both the Sp1 and NF-kappaB transcription factors at positions -295 to -286. |
| S9 | 1309-1540 | Sentence | denotes | Electrophoretic mobility shift assay (EMSA) and supershift analyses of this region documented constitutive binding of Sp1 in unactivated nuclear extracts and inducible binding of p50-p65 NF-kappaB heterodimers after P/I activation. |
| S10 | 1541-1702 | Sentence | denotes | Sp1 and NF-kappaB transcription factor binding was shown to be mutually exclusive by EMSA displacement studies with purified recombinant Sp1 and recombinant p50. |
| S11 | 1703-1998 | Sentence | denotes | The functional contribution of the kappaB-Sp1 composite site in P/I-inducible fas promoter activation was verified by using kappaB-Sp1 concatamers (-295 to -286) in a thymidine kinase promoter-driven reporter construct and native promoter constructs in Jurkat cells overexpressing IkappaB-alpha. |
| S12 | 1999-2177 | Sentence | denotes | Site-directed mutagenesis of the critical guanine nucleotides in the kappaB-Sp1 element documented the essential role of this site in activation-dependent fas promoter induction. |
GENIAcorpus
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-47 | other_name | denotes | Activation-dependent transcriptional regulation |
| T2 | 55-73 | DNA_domain_or_region | denotes | human Fas promoter |
| T3 | 83-100 | protein_complex | denotes | NF-kappaB p50-p65 |
| T4 | 114-117 | protein_molecule | denotes | Fas |
| T5 | 119-123 | protein_molecule | denotes | CD95 |
| T6 | 129-139 | protein_molecule | denotes | Fas ligand |
| T7 | 141-146 | protein_molecule | denotes | CD95L |
| T8 | 201-219 | other_name | denotes | immune homeostasis |
| T9 | 221-242 | other_name | denotes | Lymphocyte activation |
| T10 | 274-277 | protein_molecule | denotes | Fas |
| T11 | 327-331 | protein_molecule | denotes | FasL |
| T12 | 461-480 | other_name | denotes | molecular machinery |
| T13 | 563-566 | protein_molecule | denotes | Fas |
| T14 | 598-615 | other_name | denotes | mRNA accumulation |
| T15 | 617-638 | other_name | denotes | fas promoter activity |
| T16 | 644-662 | other_name | denotes | protein expression |
| T17 | 670-688 | cell_line | denotes | Jurkat T-cell line |
| T18 | 702-727 | other_organic_compound | denotes | phorbol myristate acetate |
| T19 | 732-741 | other_organic_compound | denotes | ionomycin |
| T20 | 743-746 | other_organic_compound | denotes | P/I |
| T21 | 775-790 | protein_family_or_group | denotes | T-cell receptor |
| T22 | 812-832 | cell_line | denotes | resting Jurkat cells |
| T23 | 841-844 | protein_molecule | denotes | Fas |
| T24 | 846-849 | protein_molecule | denotes | Fas |
| T25 | 901-904 | other_organic_compound | denotes | P/I |
| T26 | 924-951 | DNA_family_or_group | denotes | sequential deletion mutants |
| T27 | 959-977 | DNA_domain_or_region | denotes | human fas promoter |
| T28 | 1028-1042 | DNA_domain_or_region | denotes | 47-bp sequence |
| T29 | 1044-1066 | DNA_domain_or_region | denotes | positions -306 to -260 |
| T30 | 1083-1086 | polynucleotide | denotes | ATG |
| T31 | 1101-1135 | other_name | denotes | activation-driven fas upregulation |
| T32 | 1137-1154 | other_name | denotes | Sequence analysis |
| T33 | 1193-1228 | DNA_domain_or_region | denotes | unrecognized composite binding site |
| T34 | 1242-1245 | protein_molecule | denotes | Sp1 |
| T35 | 1250-1259 | protein_complex | denotes | NF-kappaB |
| T36 | 1260-1281 | protein_family_or_group | denotes | transcription factors |
| T37 | 1285-1307 | DNA_domain_or_region | denotes | positions -295 to -286 |
| T38 | 1309-1345 | other_name | denotes | Electrophoretic mobility shift assay |
| T39 | 1347-1351 | other_name | denotes | EMSA |
| T40 | 1427-1430 | protein_molecule | denotes | Sp1 |
| T41 | 1488-1495 | protein_complex | denotes | p50-p65 |
| T42 | 1496-1505 | protein_complex | denotes | NF-kappaB |
| T43 | 1525-1528 | other_organic_compound | denotes | P/I |
| T44 | 1541-1544 | protein_molecule | denotes | Sp1 |
| T45 | 1549-1558 | protein_complex | denotes | NF-kappaB |
| T46 | 1559-1587 | other_name | denotes | transcription factor binding |
| T47 | 1626-1630 | other_name | denotes | EMSA |
| T48 | 1678-1681 | protein_molecule | denotes | Sp1 |
| T49 | 1698-1701 | protein_subunit | denotes | p50 |
| T50 | 1738-1745 | DNA_domain_or_region | denotes | kappaB- |
| T51 | 1745-1748 | protein_molecule | denotes | Sp1 |
| T52 | 1767-1770 | other_organic_compound | denotes | P/I |
| T53 | 1834-1837 | protein_molecule | denotes | Sp1 |
| T54 | 1851-1863 | DNA_domain_or_region | denotes | -295 to -286 |
| T55 | 1870-1921 | DNA_molecule | denotes | thymidine kinase promoter-driven reporter construct |
| T56 | 1926-1952 | DNA_family_or_group | denotes | native promoter constructs |
| T57 | 1956-1968 | cell_line | denotes | Jurkat cells |
| T58 | 1984-1997 | protein_molecule | denotes | IkappaB-alpha |
| T59 | 2041-2060 | nucleotide | denotes | guanine nucleotides |
| T60 | 2068-2075 | DNA_domain_or_region | denotes | kappaB- |
| T61 | 2075-2078 | protein_molecule | denotes | Sp1 |
| T62 | 2133-2176 | other_name | denotes | activation-dependent fas promoter induction |