PMC:99051 / 6577-7177 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/99051","sourcedb":"PMC","sourceid":"99051","source_url":"https://www.ncbi.nlm.nih.gov/pmc/99051","text":"DADA distinguishes and identifies genes by their specific patterns of cDNA fragments derived from digests with 6 different restriction enzymes recognizing sequences of 4 specific nucleotides. In order to avoid detection of other fragments than the desired cDNA fragments spanning from the 3', poly-A end towards the nearest upstream restriction site, a special cloning vector has been devised. It harbors a particular combination of cleavage sites that permits cloning of a nucleic acid in defined orientation and controlled labeling of only the desired fragments with fluorescent dyes (Figs. 1,2,3).","tracks":[]}