PMC:9845374 / 40468-42178 JSONTXT

{"project":"CucurLitBase-testset_Gene","denotations":[{"id":"T31","span":{"begin":975,"end":983},"obj":"CsALC"},{"id":"T32","span":{"begin":1150,"end":1158},"obj":"CsALC"},{"id":"T33","span":{"begin":1238,"end":1246},"obj":"CsALC"},{"id":"T34","span":{"begin":1573,"end":1586},"obj":"CsALC"}],"text":"Besides the extremely high enrichment in pollens, CsRALF4/19 were also expressed in female reproductive tissues in cucumber (Fig. 3). Knockout of both CsRALF4 and CsRALF19 resulted in pollen tube rupture prematurely and reduced female fertility from diminished pollen tube distribution at lateral and terminal-TT, and decreased ovule targeting efficiency (Fig. 5). These data suggested that in addition to the conserved function in pollen tube integrity by pollen-located CsRALF4/19 peptides12,13, the transmitting tract-expressed CsRALF4/19 peptides mediate pollen tube growth and extension in the cucumber ovary, probably by interaction with pollen tube-expressed RALF receptors similar to those in pollen tube integrity control12 (Fig. 7). Expression analysis indicated the CRPs CsRALF4/19 transcripts were decreased in Csalc ovaries (Fig. 3). Although no direct interaction was found by yeast one hybrid, in vivo assay of N. benthamiana showed the positive regulation of CsALC on CsRALF4/19 expression, perhaps with the assistance of other cofactors (Supplementary Fig. 7). Interestingly, the expression of CsRALF4/19 was unchanged in the stamen of Csalc mutant (Supplementary Fig. 6), implying the positive regulations of CsRALF4/19 by CsALC is female specific. Considering the crossing data of male sterility and reduced female fertility in Csralf4 Csralf19 double mutants (Figs. 5 and 6), CsRALF4/19 may have distinct functions in male and female cells in cucumber. Notably, the phenotype of seed set decrease in Csralf4 Csralf19 double mutants was not as severe as that in Csalc mutants, indicating there are additional downstream genes of CsALC besides CsRALF4/19 during pollen tube guidance (Fig. 7)."}

{"project":"CucurLitBase-testset_BFT","denotations":[{"id":"T18","span":{"begin":542,"end":550},"obj":"BFT:2.4 functional amino acids and peptides"},{"id":"T53","span":{"begin":975,"end":983},"obj":"CsALC"},{"id":"T54","span":{"begin":1150,"end":1158},"obj":"CsALC"},{"id":"T55","span":{"begin":1238,"end":1246},"obj":"CsALC"},{"id":"T56","span":{"begin":1573,"end":1586},"obj":"CsALC"}],"text":"Besides the extremely high enrichment in pollens, CsRALF4/19 were also expressed in female reproductive tissues in cucumber (Fig. 3). Knockout of both CsRALF4 and CsRALF19 resulted in pollen tube rupture prematurely and reduced female fertility from diminished pollen tube distribution at lateral and terminal-TT, and decreased ovule targeting efficiency (Fig. 5). These data suggested that in addition to the conserved function in pollen tube integrity by pollen-located CsRALF4/19 peptides12,13, the transmitting tract-expressed CsRALF4/19 peptides mediate pollen tube growth and extension in the cucumber ovary, probably by interaction with pollen tube-expressed RALF receptors similar to those in pollen tube integrity control12 (Fig. 7). Expression analysis indicated the CRPs CsRALF4/19 transcripts were decreased in Csalc ovaries (Fig. 3). Although no direct interaction was found by yeast one hybrid, in vivo assay of N. benthamiana showed the positive regulation of CsALC on CsRALF4/19 expression, perhaps with the assistance of other cofactors (Supplementary Fig. 7). Interestingly, the expression of CsRALF4/19 was unchanged in the stamen of Csalc mutant (Supplementary Fig. 6), implying the positive regulations of CsRALF4/19 by CsALC is female specific. Considering the crossing data of male sterility and reduced female fertility in Csralf4 Csralf19 double mutants (Figs. 5 and 6), CsRALF4/19 may have distinct functions in male and female cells in cucumber. Notably, the phenotype of seed set decrease in Csralf4 Csralf19 double mutants was not as severe as that in Csalc mutants, indicating there are additional downstream genes of CsALC besides CsRALF4/19 during pollen tube guidance (Fig. 7)."}